| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,055,413 | A→G | 20.0% | D396D (GAT→GAC) | torS ← | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,055,413 | 0 | A | G | 20.0% | 80.9 / 17.4 | 40 | D396D (GAT→GAC) | torS | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
| Reads supporting (aligned to +/- strand): ref base A (19/13); new base G (5/3); total (24/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
GCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCGGGAAAGGGGAGTCAATATCAC > AP009048/1055346‑1055483 | gcgcTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGcatcc < 1:3247457/71‑1 (MQ=255) gcTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGcgtccat < 1:721450/71‑1 (MQ=255) gcTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGcatccat > 1:4097863/1‑71 (MQ=255) gcTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGcatccat < 1:705798/71‑1 (MQ=255) cTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGcgtccat < 1:237966/70‑1 (MQ=255) ttGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAg > 1:2023649/1‑71 (MQ=255) tGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAgc > 1:113310/1‑71 (MQ=255) tGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAgc > 1:469797/1‑71 (MQ=255) tGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAg < 1:4349011/70‑1 (MQ=255) aCCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAgccg < 1:3358502/71‑1 (MQ=255) aCCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAg < 1:2524050/68‑1 (MQ=255) gcgcCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAgccg > 1:3590468/1‑67 (MQ=255) cgccgcCAGCTGTTCGCGGTGGCGATTCAGTGGATGAACATTGCTGCGAAACGCGTCCATCAGCCGCCCGa > 1:1165469/1‑71 (MQ=255) gccgccAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGAt > 1:1373977/1‑71 (MQ=255) aGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCGTCCATCAGCCGCCCGATGGTATc > 1:4605718/1‑71 (MQ=255) gCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATcc < 1:1522862/71‑1 (MQ=255) cTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATc > 1:2613948/1‑69 (MQ=255) tGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCGTCCATCAGCCGCCCGATGGt < 1:3496559/65‑1 (MQ=255) ttCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCGTCCATCAGCCGCCCGa < 1:1555598/59‑1 (MQ=255) cgcgGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTcc > 1:2085831/1‑71 (MQ=255) ggTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTAt > 1:3004885/1‑60 (MQ=255) ggTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCg > 1:3644034/1‑70 (MQ=255) gTGGCGATTCAGTGCATGAACATTGCTGCGAAACGcgtccat < 1:4138349/42‑1 (MQ=255) gCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGc < 1:2123708/71‑1 (MQ=255) gCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGc < 1:1057911/71‑1 (MQ=255) gCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGc > 1:1205315/1‑71 (MQ=255) gATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGAt > 1:287745/1‑49 (MQ=255) gATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTa < 1:1226026/66‑1 (MQ=255) aTTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTACCGTACGCCAg > 1:3007469/1‑71 (MQ=255) ttCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGc > 1:264010/1‑71 (MQ=255) ttCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGc > 1:1994142/1‑71 (MQ=255) tCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTcc < 1:1952714/59‑1 (MQ=255) tCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCg > 1:2899791/1‑71 (MQ=255) tCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCg > 1:3926201/1‑71 (MQ=255) cAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGt > 1:4197041/1‑61 (MQ=255) aGTGCATGAACATTGCTGCGAAACGCGTCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGt > 1:2600341/1‑71 (MQ=255) tGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGa < 1:4097017/41‑1 (MQ=255) cATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGc < 1:801864/50‑1 (MQ=255) cATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTcc < 1:1530058/71‑1 (MQ=255) tGAACATTGCTGCGAAACGCGTCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCgg > 1:788617/1‑71 (MQ=255) gAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCgg > 1:157215/1‑70 (MQ=255) aaCATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCGGGa < 1:1387466/71‑1 (MQ=255) cATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACg < 1:1656428/53‑1 (MQ=255) tgctgcGAAACGCGTCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCGGGAAAggg > 1:367313/1‑71 (MQ=255) gctgcGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCGGGAAAggg > 1:3723464/1‑70 (MQ=255) gAAACGCGTCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCGGGaaa < 1:3045276/62‑1 (MQ=255) catccatcAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCGGGAAAGGGGAGTCaa > 1:119952/1‑66 (MQ=255) atccatcAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCGGGAAAGGGGAGTCAATATCAc > 1:4500544/1‑71 (MQ=255) | GCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTACGCCAGCGGTTTCCGGGAAAGGGGAGTCAATATCAC > AP009048/1055346‑1055483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |