| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,413,523 | G→A | 15.2% | S47F (TCC→TTC) | ydaO ← | predicted C32 tRNA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,413,523 | 0 | G | A | 15.2% | 119.0 / 11.8 | 46 | S47F (TCC→TTC) | ydaO | predicted C32 tRNA thiolase |
| Reads supporting (aligned to +/- strand): ref base G (24/15); new base A (3/4); total (27/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACG > AP009048/1413455‑1413588 | aaTTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGgag < 1:1590973/71‑1 (MQ=255) gattgGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAgg > 1:558701/1‑70 (MQ=255) ttgGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCa > 1:148710/1‑70 (MQ=255) ggCGCGCTTTGCTGCAAATTGCGCAGCATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAAc > 1:3230226/1‑71 (MQ=255) gcgcgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAAcc < 1:3544605/71‑1 (MQ=255) gcgcgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAAc > 1:3065043/1‑70 (MQ=255) cgcgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCa < 1:385461/71‑1 (MQ=255) gcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCatg < 1:3436608/70‑1 (MQ=255) cTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCatgat > 1:1891968/1‑71 (MQ=255) ttGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGc > 1:2982630/1‑71 (MQ=255) tgctgcAAATTGCGCAGAATCTCAAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACAATGATGc > 1:1413717/1‑70 (MQ=255) gctgcAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCat > 1:1346353/1‑64 (MQ=255) cAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCg > 1:3461837/1‑70 (MQ=255) aaaTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGga > 1:2614015/1‑47 (MQ=255) aaaTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAACCCATGATGCGATCGcc > 1:2224166/1‑71 (MQ=255) aaTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCct < 1:4097311/71‑1 (MQ=255) aTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCctt < 1:2820600/71‑1 (MQ=255) ttGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGcc > 1:3100232/1‑68 (MQ=255) ttGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCcttc < 1:2869855/71‑1 (MQ=255) gcgcAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCcttct < 1:4517296/70‑1 (MQ=255) gcgcAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCaaa > 1:3669681/1‑50 (MQ=255) gAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGAGATCGCCTTCTTCAAt > 1:1650448/1‑70 (MQ=255) aaTCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCttcttc < 1:88456/66‑1 (MQ=255) aTCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCa > 1:2180245/1‑70 (MQ=255) tcCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCata > 1:4611225/1‑69 (MQ=255) tcCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATAtt > 1:2287359/1‑71 (MQ=255) tcCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCg < 1:3471228/50‑1 (MQ=255) cAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTg > 1:150542/1‑70 (MQ=255) cATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGt < 1:1316951/71‑1 (MQ=255) tGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGt > 1:1375779/1‑69 (MQ=255) gTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGc < 1:3629170/71‑1 (MQ=255) ataGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCaa > 1:348866/1‑71 (MQ=255) taGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAAt < 1:4137564/71‑1 (MQ=255) gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATg < 1:3601729/70‑1 (MQ=255) cTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCata > 1:3402552/1‑54 (MQ=255) cTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCaa < 1:4338655/67‑1 (MQ=255) tCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCtt < 1:4206126/70‑1 (MQ=255) tCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCt < 1:586502/69‑1 (MQ=255) tCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTc < 1:762504/71‑1 (MQ=255) tCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCt < 1:293102/69‑1 (MQ=255) tttACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATg > 1:3915365/1‑64 (MQ=255) tttACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCg > 1:2973483/1‑70 (MQ=255) tttACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATATGATTGAAgaa > 1:1745376/1‑55 (MQ=37) tACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGcc > 1:2820244/1‑70 (MQ=255) tACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGcc > 1:1171672/1‑70 (MQ=255) aCCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGccc < 1:326326/70‑1 (MQ=255) aCCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCa > 1:1763566/1‑71 (MQ=255) ccccGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACg > 1:4499022/1‑71 (MQ=255) ggAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAg > 1:3770679/1‑50 (MQ=255) | AATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACG > AP009048/1413455‑1413588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |