| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,176,396 | C→T | 10.0% | M6I (ATG→ATA) | gatC ← | galactitol‑specific enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,176,396 | 0 | C | T | 10.0% | 149.7 / 6.3 | 50 | M6I (ATG→ATA) | gatC | galactitol‑specific enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (23/22); new base T (2/3); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
TAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGA > AP009048/2176327‑2176466 | tAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCa > 1:322469/1‑71 (MQ=255) tatTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCAt < 1:3287615/69‑1 (MQ=255) ttttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGAc < 1:1817500/70‑1 (MQ=255) tAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCt > 1:2319318/1‑71 (MQ=255) aGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCt < 1:3304500/70‑1 (MQ=255) gAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCt < 1:862076/69‑1 (MQ=255) aaaaaaTAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGaa < 1:1389348/71‑1 (MQ=255) aaaaaTAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGaa > 1:2563151/1‑70 (MQ=255) aaaaaTAATGATGACAATCGGCACCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGaa < 1:1129427/70‑1 (MQ=255) aaaaTAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGaaaa > 1:2993428/1‑71 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACa < 1:3539069/71‑1 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACa > 1:3524238/1‑71 (MQ=255) ataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACAt < 1:1372606/71‑1 (MQ=255) ataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGAc > 1:3929064/1‑59 (MQ=255) taatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATa < 1:4429274/71‑1 (MQ=255) aatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAg > 1:3819795/1‑71 (MQ=255) atgatgACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAgg > 1:2423222/1‑71 (MQ=255) atgatgACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAg < 1:416897/70‑1 (MQ=255) aaTCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTg < 1:2003615/53‑1 (MQ=255) tCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAg > 1:3842335/1‑60 (MQ=255) cGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACAt > 1:26255/1‑57 (MQ=255) cGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACAt > 1:3419062/1‑57 (MQ=255) ggCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTAt < 1:1724402/41‑1 (MQ=255) gcagcaTCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAg < 1:2148858/71‑1 (MQ=255) caTCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACAt < 1:3663933/51‑1 (MQ=255) caTCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAg < 1:3538573/71‑1 (MQ=255) caTCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAg < 1:789394/71‑1 (MQ=255) aTCACCGTAGGGCCTAGGTCGAGAATATAACGTATGACTTCTGaaa < 1:1185530/46‑1 (MQ=255) tCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACt < 1:4563643/38‑1 (MQ=255) tCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGt > 1:2628087/1‑70 (MQ=255) cACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGaaaa < 1:3708812/45‑1 (MQ=255) cACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGt > 1:2472390/1‑69 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACAt > 1:1239529/1‑47 (MQ=255) ccGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGa > 1:3664190/1‑63 (MQ=255) gggCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAatttt < 1:2647448/71‑1 (MQ=255) cGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAg < 1:4520362/53‑1 (MQ=255) cGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattttattt < 1:1291244/71‑1 (MQ=255) cGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattttattt > 1:1621200/1‑71 (MQ=255) ggTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTaa < 1:1801756/49‑1 (MQ=255) gTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGt > 1:3639063/1‑70 (MQ=255) cGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAAt > 1:3856105/1‑71 (MQ=255) gagaATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATg > 1:1016207/1‑71 (MQ=255) gaATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCa > 1:3784886/1‑51 (MQ=255) gaATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCt > 1:3448087/1‑71 (MQ=255) gaATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCt > 1:2985448/1‑71 (MQ=255) gaATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCt > 1:949859/1‑71 (MQ=255) taACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattttattt > 1:2074690/1‑57 (MQ=255) taACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattttatt > 1:3487657/1‑56 (MQ=255) aCGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAattt < 1:15054/50‑1 (MQ=255) gCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTcgataccg < 1:996981/71‑1 (MQ=255) cATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTcgataccga < 1:579307/71‑1 (MQ=255) | TAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGA > AP009048/2176327‑2176466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |