| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,688,553 | A→T | 7.8% | V401D (GTC→GAC) | yfhK ← | predicted sensory kinase in two‑component system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,688,553 | 0 | A | T | 7.8% | 141.5 / 4.4 | 51 | V401D (GTC→GAC) | yfhK | predicted sensory kinase in two‑component system |
| Reads supporting (aligned to +/- strand): ref base A (25/22); new base T (2/2); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
CCCTGAAAAAAGGGTTCGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGTTACCGGATTCAGCCCCGTAGTG > AP009048/2688487‑2688623 | cccTGAAAAAAGGGTTCGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACAt > 1:1523368/1‑70 (MQ=255) ccTGAAAAAAGGGTTCGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATc < 1:1179184/70‑1 (MQ=255) cTGAAAAAAGGGTTCGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCaa < 1:1703745/71‑1 (MQ=255) aaaaaaGGGTTCGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAata > 1:354780/1‑70 (MQ=255) aaaaGGGTTCGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATaa < 1:2131916/71‑1 (MQ=255) aaGGGTTCGAAGATCATGTCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAatat < 1:127500/67‑1 (MQ=255) aaGGGTTCGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAAc > 1:3315279/1‑71 (MQ=255) gTTCGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCcgc < 1:4048543/71‑1 (MQ=255) cGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAAcc > 1:1988788/1‑65 (MQ=255) gAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAAtata > 1:1897172/1‑60 (MQ=255) gATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATaa < 1:112099/58‑1 (MQ=255) aTCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAat < 1:909903/53‑1 (MQ=255) aTCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAat < 1:1439000/53‑1 (MQ=255) cATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGt > 1:4522996/1‑70 (MQ=255) ggCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACAt > 1:2850628/1‑44 (MQ=255) ggCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAAc > 1:2587014/1‑71 (MQ=255) gcgTTCCTCTTGCGGAATGGGCGTGCCTATATTGATGTCATCAata < 1:3557183/46‑1 (MQ=37) gcgTTCCTCTTGCGGAATGGGCGTGCCTATATTGATGTCATCAata < 1:3551969/46‑1 (MQ=37) cgTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAActgct > 1:2718241/1‑71 (MQ=255) ttCCTCTTGCGGAATGGGCGTGCCTATATTGATGTCATCAatac > 1:862129/1‑43 (MQ=25) ccTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCcgcg > 1:342960/1‑51 (MQ=255) ccTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTa < 1:1418824/60‑1 (MQ=255) ccTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCg > 1:719259/1‑70 (MQ=255) ccTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCg > 1:1535988/1‑70 (MQ=255) ctctTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATaa < 1:3215536/43‑1 (MQ=255) tctTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAAcc < 1:3097530/45‑1 (MQ=255) ttGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAgg > 1:363317/1‑70 (MQ=255) ttGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAg < 1:1564290/69‑1 (MQ=255) tGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGc > 1:3877049/1‑70 (MQ=255) gCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAAcc > 1:1871865/1‑41 (MQ=255) gCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCa < 1:73166/70‑1 (MQ=255) cGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCTCACCATGTAAACTGCTGCGAAGGCaa < 1:540950/70‑1 (MQ=255) cGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCaaa > 1:3711723/1‑71 (MQ=255) cGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCaa < 1:4452783/70‑1 (MQ=255) ggAATGGGCGTGCCTGTATTGATGACATCAATATAAAc > 1:954586/1‑38 (MQ=255) ggAATGGGCGTCCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACtgctgc < 1:3645332/61‑1 (MQ=255) aTGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGtt < 1:3350244/71‑1 (MQ=255) aTGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGt > 1:1570752/1‑70 (MQ=255) tGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGTTa > 1:4106331/1‑71 (MQ=255) gCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGa < 1:2675859/56‑1 (MQ=255) cGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTaaa > 1:887739/1‑46 (MQ=255) gTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAg > 1:73379/1‑56 (MQ=255) tGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCg < 1:3664415/52‑1 (MQ=255) tGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGTTACCGGAt > 1:1784830/1‑71 (MQ=255) ccTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGaa > 1:4059537/1‑52 (MQ=255) ccTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGTTACCGGAtt < 1:1194562/70‑1 (MQ=255) tGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTCCTGCGAAGGCAAATGtt < 1:3184120/60‑1 (MQ=255) gTATTGATGACATCAATATAAACCCGCGCACCATGTAAActgctg > 1:3203697/1‑45 (MQ=255) gTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAAt > 1:1383302/1‑56 (MQ=255) atATTGATGTCATCAATACAAACCCGCGCACCATGTaaa > 1:1104406/2‑39 (MQ=21) aTTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGTTACCGGAtt < 1:4447881/65‑1 (MQ=255) gatgaCATCAATATAAACCCGCGCACCATGTAAACTGCTGCGaa > 1:3824487/1‑44 (MQ=255) atgaCATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGTTACCGGAt < 1:473301/60‑1 (MQ=255) tgaCATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGTTACCGGa < 1:3108881/58‑1 (MQ=255) aCATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGTTACCGGATTCAGCCCCGTAGTg < 1:1240541/71‑1 (MQ=255) | CCCTGAAAAAAGGGTTCGAAGATCATGGCGCGTTCCTCTTGCGGAATGGGCGTGCCTGTATTGATGACATCAATATAAACCCGCGCACCATGTAAACTGCTGCGAAGGCAAATGTTACCGGATTCAGCCCCGTAGTG > AP009048/2688487‑2688623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |