| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,719,037 | G→A | 17.1% | V143V (GTG→GTA) | yfiQ → | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,719,037 | 0 | G | A | 17.1% | 100.2 / 12.8 | 41 | V143V (GTG→GTA) | yfiQ | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/19); new base A (3/4); total (18/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACT > AP009048/2718968‑2719104 | ccTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGc < 1:2613357/71‑1 (MQ=255) cTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGcc < 1:2642010/71‑1 (MQ=255) cTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGcc < 1:1015881/71‑1 (MQ=255) tGCTTGGACCAAACAGTATGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGcc > 1:2238965/1‑70 (MQ=255) ggACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:1045626/70‑1 (MQ=255) ggACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:606862/70‑1 (MQ=255) ccAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAcg > 1:355998/1‑71 (MQ=255) aaaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAAcgcg < 1:4059241/71‑1 (MQ=255) aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:3921294/64‑1 (MQ=255) aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCgg > 1:4301439/1‑71 (MQ=255) aCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCgg > 1:4346841/1‑70 (MQ=255) aCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGAATAAAcgcg > 1:4306092/1‑69 (MQ=255) tCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGc < 1:3897611/71‑1 (MQ=255) tGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTg > 1:1594585/1‑71 (MQ=255) tACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAcgc > 1:2007754/1‑56 (MQ=255) tACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGtt > 1:1088705/1‑70 (MQ=255) ggCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTAtt > 1:672736/1‑70 (MQ=255) gCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTAtt < 1:4581609/69‑1 (MQ=255) ccTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCt < 1:3342275/55‑1 (MQ=255) cTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt < 1:206590/71‑1 (MQ=255) tGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGAt < 1:903232/38‑1 (MQ=255) gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATc < 1:2290292/69‑1 (MQ=255) gCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCg > 1:3269524/1‑70 (MQ=255) aaGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:2574635/36‑1 (MQ=255) ggTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTAt < 1:1629961/56‑1 (MQ=255) tCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAc < 1:2522459/35‑1 (MQ=255) cTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTAtt > 1:3201846/1‑54 (MQ=255) cTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGtt < 1:1292151/50‑1 (MQ=255) aaTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtctc > 1:1145792/1‑71 (MQ=255) aaTGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtctc > 1:3877211/1‑71 (MQ=255) aTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTcc < 1:2596342/71‑1 (MQ=255) tGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtctc > 1:3017399/1‑69 (MQ=255) ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCaa < 1:1871908/70‑1 (MQ=255) cAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtct < 1:2621484/65‑1 (MQ=255) aGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtct > 1:2954945/1‑64 (MQ=255) gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAacac > 1:2358301/1‑70 (MQ=255) cTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCa < 1:4151577/71‑1 (MQ=255) cTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCa < 1:1643067/71‑1 (MQ=255) ttttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAt > 1:3489392/1‑71 (MQ=255) tCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCt > 1:1809167/1‑71 (MQ=255) cTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGAc < 1:2106886/71‑1 (MQ=255) tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACt < 1:2451593/69‑1 (MQ=255) | CCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACT > AP009048/2718968‑2719104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |