| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,863,059 | G→A | 11.5% | L56L (CTG→CTA) | ygbM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,863,059 | 0 | G | A | 11.5% | 177.4 / 11.6 | 61 | L56L (CTG→CTA) | ygbM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (29/25); new base A (4/3); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
AATTTCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCT > AP009048/2862989‑2863118 | aaTTTCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTg > 1:504777/1‑71 (MQ=255) tCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGgcgc > 1:3215477/1‑71 (MQ=255) tttCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTg < 1:3955103/69‑1 (MQ=255) tCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTc < 1:4054656/70‑1 (MQ=255) tCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTc < 1:2169622/70‑1 (MQ=255) ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa < 1:2510490/70‑1 (MQ=255) ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa < 1:3965409/70‑1 (MQ=255) ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCaa < 1:3905617/70‑1 (MQ=255) cTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATa < 1:4028167/71‑1 (MQ=255) taACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCt > 1:391971/1‑60 (MQ=255) ctCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCg < 1:3187711/71‑1 (MQ=255) ctCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCg < 1:3449549/71‑1 (MQ=255) ctCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCg < 1:3475488/71‑1 (MQ=255) tCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAAt < 1:3875618/60‑1 (MQ=255) ccACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCg > 1:547256/1‑69 (MQ=255) cACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGgc > 1:3175589/1‑48 (MQ=255) cACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCg > 1:661168/1‑68 (MQ=255) cACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCg > 1:2876889/1‑68 (MQ=255) cccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGat < 1:2214689/71‑1 (MQ=255) cccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCa > 1:4179077/1‑54 (MQ=255) cTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGatat > 1:504806/1‑71 (MQ=255) tGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGatat > 1:56226/1‑70 (MQ=255) tGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACAGCTCCCGGAGatat > 1:3396919/1‑70 (MQ=255) tGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCaa < 1:2088820/52‑1 (MQ=255) gCAAATCCAAAAGCAACTGTAGCAAAACCATCTGACACTGgc < 1:2548985/42‑1 (MQ=255) gCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATa > 1:190208/1‑53 (MQ=255) cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTaa > 1:1335363/1‑71 (MQ=255) aaaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa < 1:107024/49‑1 (MQ=255) aaaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGgaga > 1:2012831/1‑64 (MQ=255) aaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATAc > 1:3879732/1‑51 (MQ=255) aTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCg > 1:2717397/1‑58 (MQ=255) aTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg > 1:4409846/1‑70 (MQ=255) aTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGc > 1:1929314/1‑71 (MQ=255) tCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCg > 1:2098123/1‑71 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATAc < 1:1215284/48‑1 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATAc < 1:4314682/48‑1 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATAc < 1:146114/48‑1 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:9667/64‑1 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg < 1:127533/68‑1 (MQ=255) cAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATTCCGCTCCCGGAGATATTAATGCgg < 1:800572/70‑1 (MQ=255) aaaaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGata > 1:4616457/1‑60 (MQ=255) aaaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa < 1:763844/42‑1 (MQ=255) aaaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGata > 1:1577564/1‑59 (MQ=255) aaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATAc > 1:4575496/1‑44 (MQ=255) aaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCggggg > 1:4067162/1‑70 (MQ=255) aaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCggg > 1:1292550/1‑68 (MQ=255) aaGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCgggg > 1:1522514/1‑69 (MQ=255) aaCTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATgg > 1:3295709/1‑71 (MQ=255) aCTGGAGCAAATCCATCTGACACTGGCGCTGTTCaa < 1:2982588/36‑1 (MQ=255) aCTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa < 1:2988082/36‑1 (MQ=255) aCTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATggg < 1:265502/71‑1 (MQ=255) cTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATggg > 1:1888387/1‑70 (MQ=255) ggAGCAAAACCATCTGACACTGGCGCTGTTCAATa > 1:2196779/1‑35 (MQ=255) ggAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCgg > 1:3197733/1‑59 (MQ=255) gAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg > 1:2390600/1‑55 (MQ=255) gAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg > 1:2396044/1‑55 (MQ=255) aGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGaa > 1:1208514/1‑62 (MQ=255) gCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCg < 1:1388938/55‑1 (MQ=255) aaaCCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCg < 1:3599677/71‑1 (MQ=255) aTCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCt < 1:2942514/70‑1 (MQ=255) cTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGc > 1:3591900/1‑44 (MQ=255) tGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGcc > 1:103271/1‑59 (MQ=255) | AATTTCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCT > AP009048/2862989‑2863118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |