| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,001,511 | T→C | 12.8% | I81T (ATA→ACA) | xdhB → | xanthine dehydrogenase, FAD‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,001,511 | 0 | T | C | 12.8% | 203.6 / 21.1 | 78 | I81T (ATA→ACA) | xdhB | xanthine dehydrogenase, FAD‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (35/33); new base C (6/4); total (41/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
GGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCGC > AP009048/3001447‑3001579 | ggAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATagaaga < 1:2438259/71‑1 (MQ=255) aTTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATcc > 1:1203919/1‑71 (MQ=255) aTTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATcc > 1:3667450/1‑71 (MQ=255) aCGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCta > 1:62726/1‑70 (MQ=255) cGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCtat < 1:3632715/70‑1 (MQ=255) gCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATaga < 1:2394586/60‑1 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATagaag > 1:2427013/1‑58 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATaa > 1:43117/1‑67 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACt < 1:940662/69‑1 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACt < 1:1359690/69‑1 (MQ=255) gAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACg > 1:3542838/1‑71 (MQ=255) gAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACg > 1:2299828/1‑71 (MQ=255) gAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACg > 1:2000527/1‑71 (MQ=255) aaGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGt > 1:3394307/1‑71 (MQ=255) aaGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGt > 1:4104343/1‑71 (MQ=255) aGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTc < 1:4497874/71‑1 (MQ=255) aTGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATaa > 1:2512103/1‑60 (MQ=255) aTGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCa > 1:2166692/1‑70 (MQ=255) tGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCtat < 1:3699276/57‑1 (MQ=255) ggCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATagaa > 1:30018/1‑48 (MQ=255) gCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAAc < 1:4594342/58‑1 (MQ=255) gCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCAtctc > 1:1661644/1‑71 (MQ=255) tCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTccc > 1:4421704/1‑71 (MQ=255) tCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTccc > 1:664464/1‑71 (MQ=255) cGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCg > 1:2354704/1‑71 (MQ=255) gCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCa > 1:3439831/1‑63 (MQ=255) gCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCa > 1:257541/1‑63 (MQ=255) gCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCg < 1:1926425/70‑1 (MQ=255) gCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCg > 1:4312536/1‑70 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCg < 1:2035629/69‑1 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt > 1:2201008/1‑70 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt > 1:3878452/1‑70 (MQ=255) tATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAt < 1:1648099/71‑1 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACg < 1:323112/52‑1 (MQ=255) ggCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCg > 1:2978364/1‑60 (MQ=255) gCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgct < 1:4271233/71‑1 (MQ=255) ctctGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgctg < 1:2487541/71‑1 (MQ=255) tctGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgctgc > 1:2766226/1‑71 (MQ=255) tGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgctgc < 1:2054249/69‑1 (MQ=255) tGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgctgc < 1:3710979/69‑1 (MQ=255) tGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgctg < 1:1411904/68‑1 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGtt < 1:4435871/58‑1 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa < 1:890478/59‑1 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc < 1:2463729/70‑1 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCATTATGTGCTGCGGc > 1:3999248/1‑70 (MQ=255) aaCGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCa > 1:3927343/1‑71 (MQ=255) aCGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCAc > 1:438985/1‑71 (MQ=255) cGACATTTAACCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACg < 1:1814874/71‑1 (MQ=255) gACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGt < 1:1059063/71‑1 (MQ=255) aCATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTc > 1:1111358/1‑71 (MQ=255) cATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTc > 1:2019081/1‑70 (MQ=255) aTTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCAtctc < 1:3970944/43‑1 (MQ=255) aTTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCa > 1:3884713/1‑71 (MQ=255) tttACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgctg < 1:3810932/58‑1 (MQ=255) tttACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCAt < 1:1024008/71‑1 (MQ=255) ttACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCAtt > 1:1274118/1‑71 (MQ=255) ttACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCAtt > 1:3243733/1‑71 (MQ=255) ttACCCAGCTAACAGAAGATCCTATAACTCAACGTAATCTCCCGGCGTTATGTGCTGCGGCCACGTCCAtt > 1:2319128/1‑71 (MQ=255) aCCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTg < 1:4204096/70‑1 (MQ=255) cccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGc < 1:3558173/70‑1 (MQ=255) ccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCg < 1:4427116/43‑1 (MQ=255) ccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTg > 1:1448323/1‑71 (MQ=255) ccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTg < 1:842646/71‑1 (MQ=255) ccAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTg > 1:3526534/1‑71 (MQ=255) aGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:3702078/1‑55 (MQ=255) aGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGa < 1:3520220/71‑1 (MQ=255) aGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGa > 1:4284851/1‑71 (MQ=255) gCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGtt < 1:3206921/42‑1 (MQ=255) cTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGc > 1:659761/1‑65 (MQ=255) cTAATAGAAGATCCTATAACTCAACGTCATCTCCC‑GCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg < 1:2708059/71‑1 (MQ=255) tAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGAcc < 1:4306652/70‑1 (MQ=255) tAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTg > 1:2855867/1‑66 (MQ=255) aaTAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc < 1:4352437/51‑1 (MQ=255) aaTAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGa > 1:3512553/1‑67 (MQ=255) aaTAGAAGATCCTATAACTCAACGTAATCTCCCGGCGtt > 1:4001024/1‑39 (MQ=255) aTAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg < 1:1647177/69‑1 (MQ=255) aTAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg < 1:1356029/69‑1 (MQ=255) aTAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCGc < 1:3300609/70‑1 (MQ=255) | GGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCGC > AP009048/3001447‑3001579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |