| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,018,853 | C→T | 12.0% | T346I (ACT→ATT) | ssnA → | predicted chlorohydrolase/aminohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,018,853 | 0 | C | T | 12.0% | 217.0 / 16.5 | 75 | T346I (ACT→ATT) | ssnA | predicted chlorohydrolase/aminohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (27/39); new base T (5/4); total (32/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
AATTTGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGTTACAAAGC > AP009048/3018784‑3018922 | aaTTTGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGAc > 1:988208/1‑70 (MQ=255)aaTTTGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGAc > 1:326983/1‑70 (MQ=255) tttGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTaa < 1:1720363/71‑1 (MQ=255) tttGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTa < 1:3361574/70‑1 (MQ=255) ttGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAAc > 1:2388245/1‑71 (MQ=255) tGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACg > 1:1960494/1‑71 (MQ=255) ccTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACt < 1:1563754/65‑1 (MQ=255) cttcttTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTa < 1:1954775/71‑1 (MQ=255) cttTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAAc > 1:3094648/1‑70 (MQ=255) cttTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAAc > 1:1202122/1‑70 (MQ=255) cttTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAAc > 1:3041716/1‑70 (MQ=255) cttTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAAc > 1:4568361/1‑70 (MQ=255) tttAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACg > 1:1273635/1‑70 (MQ=255) tAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACGAAc > 1:2797116/1‑71 (MQ=255) tAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACGAAc > 1:3905561/1‑71 (MQ=255) tAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACg < 1:3207183/68‑1 (MQ=255) aCATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAAc < 1:3081255/64‑1 (MQ=255) aTCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACtgatg < 1:518658/71‑1 (MQ=255) aTCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACtgatg < 1:3035840/71‑1 (MQ=255) gATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCg > 1:1558145/1‑71 (MQ=255) tGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTaa < 1:3142445/48‑1 (MQ=255) gggTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACt < 1:4293802/71‑1 (MQ=255) gggTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACt < 1:3004291/71‑1 (MQ=255) ggtggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACtt < 1:3417699/71‑1 (MQ=255) ggtggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACtt < 1:3879978/71‑1 (MQ=255) tggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACttt < 1:3827278/70‑1 (MQ=255) tggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTg < 1:4408814/71‑1 (MQ=255) tggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTg < 1:1145689/71‑1 (MQ=255) tggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTg < 1:1064038/71‑1 (MQ=255) tggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTg < 1:1246175/71‑1 (MQ=255) gtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCa < 1:4295700/63‑1 (MQ=255) tCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAg < 1:2209181/57‑1 (MQ=255) tCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTg < 1:4164708/68‑1 (MQ=255) ccGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCa < 1:2627337/61‑1 (MQ=255) ccGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGtcgc > 1:4384123/1‑71 (MQ=255) cGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGc > 1:515774/1‑59 (MQ=255) gCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACttt > 1:1732185/1‑64 (MQ=255) cTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACg < 1:737493/43‑1 (MQ=255) gtgGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAg < 1:697496/51‑1 (MQ=255) gtgGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGcc > 1:2590561/1‑53 (MQ=255) tgGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGaaa < 1:701557/69‑1 (MQ=255) tgGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACtgatg > 1:545550/1‑48 (MQ=255) tgGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAtt > 1:2073177/1‑71 (MQ=255) gCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTgg > 1:4115481/1‑60 (MQ=255) gCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTg > 1:1948053/1‑59 (MQ=255) gCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGgc > 1:2575966/1‑61 (MQ=255) gCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGgc > 1:3440750/1‑61 (MQ=255) gCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAttt < 1:3072002/70‑1 (MQ=255) gCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAttt < 1:3317542/70‑1 (MQ=255) ccTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAtt < 1:3599136/68‑1 (MQ=255) cTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACg < 1:714094/36‑1 (MQ=255) cTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAAc < 1:1669045/39‑1 (MQ=255) gACAGTTTTGCCAAAGCCCTGACTAACGGTAACGaa > 1:2408982/1‑36 (MQ=255) aCAGTTTTGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCtt < 1:1750681/71‑1 (MQ=255) aCAGTTTTGCCAAAGCCCTGACTAACGGTAACGaa > 1:2544153/1‑35 (MQ=255) aCAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGc > 1:728118/1‑44 (MQ=255) aCAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACttt < 1:1836442/53‑1 (MQ=255) gTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTg < 1:1744559/71‑1 (MQ=255) ttttGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGcgcg < 1:1924955/55‑1 (MQ=255) ttttGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCt < 1:4076770/66‑1 (MQ=255) ttttGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTgg < 1:2907473/71‑1 (MQ=255) tGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGaa < 1:924055/70‑1 (MQ=255) gCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGc > 1:3113670/1‑71 (MQ=255) ccAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAtt < 1:2588170/55‑1 (MQ=255) cAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAtt < 1:3823882/54‑1 (MQ=255) aaGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTg < 1:4162267/54‑1 (MQ=255) aaGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGt > 1:2556206/1‑71 (MQ=255) aaGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGt > 1:1461047/1‑71 (MQ=255) aGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGt > 1:20850/1‑70 (MQ=255) aGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGt > 1:4530740/1‑70 (MQ=255) aGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGt > 1:754618/1‑70 (MQ=255) cccTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAtt < 1:3793393/49‑1 (MQ=255) cccTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCt < 1:4358823/58‑1 (MQ=255) ccTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTgg < 1:135320/51‑1 (MQ=255) cTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGgc > 1:710522/1‑39 (MQ=255) cTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGgc > 1:854666/1‑39 (MQ=255) aCTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGTTACAAAGc > 1:3679029/1‑71 (MQ=255) | AATTTGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGTTACAAAGC > AP009048/3018784‑3018922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |