Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,141,208:1	+T	6.8%	coding (168/264 nt)	cedA ←	cell division modulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,141,208	1	.	T	6.8%	282.2 / 18.7	74	coding (168/264 nt)	cedA	cell division modulator
Reads supporting (aligned to +/- strand):  ref base . (42/27);  new base T (2/3);  total (44/30)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CTGATTTGCCCAACGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATCTTTATCG  >  Exported/1141142‑1141275                                                                    |                                                                    cTGATTTGCCCAACGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑At                                                                   >  1:2767650/1‑69 (MQ=255) cTGATTTGCCCAACGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑At                                                                   >  1:1573403/1‑69 (MQ=255)  tGATTTGCCCAACGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAg                                                                >  1:1119938/1‑71 (MQ=255)    aTTTGCCCAACGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTa                                                              >  1:3566936/1‑71 (MQ=255)     tttGCCCAACGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAg                                                                <  1:2772425/68‑1 (MQ=255)        gCCCAACGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACaa                                                           <  1:3149901/70‑1 (MQ=255)            aaCGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCCTATCAGTACAAACg                                                        >  1:2410171/1‑70 (MQ=255)            aaCGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGc                                                       >  1:2745355/1‑70 (MQ=255)             aCGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCaa                                                     <  1:3336228/71‑1 (MQ=255)             aCGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCaa                                                     >  1:1204905/1‑71 (MQ=255)             aCGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCaa                                                     <  1:1703156/71‑1 (MQ=255)              cGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCaa                                                     >  1:2333216/1‑70 (MQ=255)                   ggCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCCTATCAGTACAAACGCAACatat                                                <  1:1183826/71‑1 (MQ=255)                   ggCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCCTATCAGTACAAACGCAACatat                                                <  1:280045/71‑1 (MQ=255)                   ggCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCCCCCTATCAGTACAAACGCAACatat                                                <  1:283462/71‑1 (MQ=255)                     cAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTg                                             >  1:2101486/1‑71 (MQ=255)                       gATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTCGc                                            >  1:556179/1‑70 (MQ=255)                          taaGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCAc                                         >  1:1125811/3‑70 (MQ=255)                             ggCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCa                                                      <  1:347719/54‑1 (MQ=255)                              gCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACgcag                                     <  1:2478987/70‑1 (MQ=255)                                 caGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATc                                 <  1:3155329/71‑1 (MQ=255)                                 caGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATc                                 <  1:2683828/71‑1 (MQ=255)                                      aaCGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCa                                                      <  1:316209/45‑1 (MQ=255)                                         gCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGc                                            >  1:1534169/1‑52 (MQ=255)                                         gCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGc                                            >  1:776803/1‑52 (MQ=255)                                           cGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCac                       >  1:2662006/1‑71 (MQ=255)                                             gTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACa                                                   <  1:2976932/41‑1 (MQ=255)                                             gTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACa                                                   <  1:2992796/41‑1 (MQ=255)                                             gTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACa                                                   <  1:1885958/41‑1 (MQ=255)                                             gTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacga                     <  1:2241590/71‑1 (MQ=255)                                             gTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacga                     <  1:3852239/71‑1 (MQ=255)                                             gTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacga                     <  1:441396/71‑1 (MQ=255)                                              tGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACa                                                   <  1:813549/40‑1 (MQ=255)                                              tGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGAc                            <  1:2001513/63‑1 (MQ=255)                                                  cgcAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacgaaacga                <  1:582717/71‑1 (MQ=255)                                                  cgcAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacgaaacga                <  1:2633148/71‑1 (MQ=255)                                                   gcAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacgaaacgaa               >  1:3542329/1‑71 (MQ=255)                                                    cAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGAc                            >  1:2843464/1‑57 (MQ=255)                                                    cAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACAt                          >  1:1651660/1‑59 (MQ=255)                                                    cAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACAt                          >  1:2865633/1‑59 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACgc                                       >  1:908800/1‑45 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACgc                                       >  1:3463591/1‑45 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACgc                                       >  1:487608/1‑45 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacga                     <  1:3429781/63‑1 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacga                     <  1:1109812/63‑1 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacga                     <  1:503255/63‑1 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacga                     <  1:662125/63‑1 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacga                     <  1:1753010/63‑1 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATc             >  1:2375529/1‑71 (MQ=255)                                                     aGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATc             >  1:2187558/1‑71 (MQ=255)                                                      gAAATGACTCTCCC‑ATCAGTACAAACGCAACATAtt                                               >  1:3240579/1‑36 (MQ=255)                                                      gAAATGACTCTCCC‑ATCAGTACAAACGCAACATAtt                                               >  1:3245024/1‑36 (MQ=255)                                                       aaaTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGa                             <  1:2117696/53‑1 (MQ=255)                                                       aaaTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacgaaa                   >  1:3141152/1‑63 (MQ=255)                                                       aaaTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacgaaa                   >  1:3575930/1‑63 (MQ=255)                                                       aaaTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacgaaa                   >  1:1794274/1‑63 (MQ=255)                                                       aaaTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCacgaaa                   >  1:1936727/1‑63 (MQ=255)                                                       aaaTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATcc            <  1:1614383/70‑1 (MQ=255)                                                       aaaTGACTCTCCC‑ATCAGTAC‑AACGCAACATATTTGCCACGCAGCATCCAGa                             <  1:1186973/52‑1 (MQ=255)                                                         aTGACTCTCCCTATCAGTACAAACGCAACATATTTGCCACGCGGCATCCAGACATCACGAAACGAATCCAt          >  1:594800/1‑71 (MQ=255)                                                         aTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATc         >  1:998623/1‑71 (MQ=255)                                                         aTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATc         >  1:1047281/1‑71 (MQ=255)                                                         aTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATc         >  1:167890/1‑71 (MQ=255)                                                         aTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATc         >  1:1887788/1‑71 (MQ=255)                                                         aTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATc         >  1:2729254/1‑71 (MQ=255)                                                         aTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATc         >  1:329257/1‑71 (MQ=255)                                                         aTGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACCAATCCATc         >  1:997361/1‑71 (MQ=255)                                                                tcCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATCTTTATCg  >  1:3807699/1‑71 (MQ=255)                                                                tcCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATCTTTATCg  >  1:2554950/1‑71 (MQ=255)                                                                tcCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATCTTTATCg  >  1:2620888/1‑71 (MQ=255)                                                                tcCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATCTTTATCg  >  1:654582/1‑71 (MQ=255)                                                                tcCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATCTTTATCg  >  1:2629768/1‑71 (MQ=255)                                                                tcCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATCTTTATCg  >  1:2918690/1‑71 (MQ=255)                                                                tcCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATCTTTATCg  >  1:2924257/1‑71 (MQ=255)                                                                    |                                                                    CTGATTTGCCCAACGTTGGGCAGATTCAGGCACAGTAAACGCCGGTGAGCGCAGAAATGACTCTCCC‑ATCAGTACAAACGCAACATATTTGCCACGCAGCATCCAGACATCACGAAACGAATCCATCTTTATCG  >  Exported/1141142‑1141275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑