Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,892,0191.G100.0% 187.8 / NA 52intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/26);  total (26/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*Exported1,892,0192.C100.0% 187.9 / NA 52intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/26);  total (26/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  Exported/1891961‑1892077
                                                           ||                                                          
ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:758274/71‑1 (MQ=255)
ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:1689156/71‑1 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               >  1:2820272/1‑70 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               <  1:475012/70‑1 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                <  1:3249877/69‑1 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                <  1:3795687/69‑1 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg                                              >  1:1066714/1‑71 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg                                              >  1:1801551/1‑71 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg                                              >  1:1720669/1‑71 (MQ=255)
     aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg                                             >  1:3540778/1‑71 (MQ=255)
        gattcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc                                          <  1:3834663/71‑1 (MQ=255)
         attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         <  1:2543757/71‑1 (MQ=255)
           tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       >  1:1087296/1‑71 (MQ=255)
           tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       >  1:2335846/1‑71 (MQ=255)
              attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                                    <  1:3302031/71‑1 (MQ=255)
                  aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc                                 >  1:1256370/1‑70 (MQ=255)
                    cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  <  1:866142/67‑1 (MQ=255)
                    cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt                              >  1:251509/1‑71 (MQ=255)
                      ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCgg                                        <  1:2954944/59‑1 (MQ=255)
                       cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg                                             >  1:2944972/1‑53 (MQ=255)
                        gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca                                                    <  1:2126453/45‑1 (MQ=255)
                        gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca                                                    <  1:1665679/45‑1 (MQ=255)
                        gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgcg                          >  1:3166837/1‑71 (MQ=255)
                         cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         >  1:1365911/1‑71 (MQ=255)
                          tAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       >  1:385502/1‑56 (MQ=255)
                          tAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     >  1:153901/1‑58 (MQ=255)
                          tAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     >  1:2946047/1‑58 (MQ=255)
                           aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:8153/44‑1 (MQ=255)
                           aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:991163/44‑1 (MQ=255)
                            aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                       <  1:603452/70‑1 (MQ=255)
                              aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAt                    >  1:430574/1‑71 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGaa                                                 >  1:663535/1‑41 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:1455040/1‑42 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:2140285/1‑42 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:2131643/1‑42 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:2023354/1‑42 (MQ=255)
                                gACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATcc                  <  1:1662974/71‑1 (MQ=255)
                                 aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATATGACGTTCGCGTCTCATCCg                 <  1:3107286/71‑1 (MQ=255)
                                 aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg                 <  1:2535161/71‑1 (MQ=255)
                                   aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                                    <  1:1515447/50‑1 (MQ=255)
                                   aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                                    <  1:1225412/50‑1 (MQ=255)
                                      tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg                 >  1:920476/1‑66 (MQ=255)
                                      tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt             >  1:2392108/1‑70 (MQ=255)
                                          gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTg        <  1:805717/71‑1 (MQ=39)
                                           ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGAc               >  1:1172239/1‑63 (MQ=38)
                                           ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGt       >  1:438246/1‑71 (MQ=255)
                                              gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt     <  1:3809039/70‑1 (MQ=38)
                                              gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt     <  1:2146530/70‑1 (MQ=38)
                                               ctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                       <  1:2801588/51‑1 (MQ=255)
                                                tGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  >  1:1737316/1‑39 (MQ=25)
                                                  aaCATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTg            <  1:3447525/59‑1 (MQ=38)
                                                  aaCATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAc  <  1:104575/69‑1 (MQ=38)
                                                           ||                                                          
CCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  Exported/1891961‑1892077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: