| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 40,322 | C→T | 5.8% | P81L (CCG→CTG) | fixA → | predicted electron transfer flavoprotein subunit, ETFP adenine nucleotide‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 40,322 | 0 | C | T | 5.8% | 288.5 / 3.9 | 85 | P81L (CCG→CTG) | fixA | predicted electron transfer flavoprotein subunit, ETFP adenine nucleotide‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (39/41); new base T (2/3); total (41/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACT > Exported/40253‑40391 | cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg < 1:3608493/71‑1 (MQ=255)cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg < 1:3101549/71‑1 (MQ=255) aGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCgg < 1:3547242/71‑1 (MQ=255) ccTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGAt > 1:2583213/1‑71 (MQ=255) ccTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGAt > 1:730682/1‑71 (MQ=255) cTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATg < 1:374433/71‑1 (MQ=255) tgGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGAtt < 1:988380/71‑1 (MQ=255) gggCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg > 1:3288738/1‑71 (MQ=255) ggCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgt < 1:2091055/71‑1 (MQ=255) ggCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgt < 1:1795344/71‑1 (MQ=255) ggCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgt < 1:2869222/71‑1 (MQ=255) ggCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgt < 1:100652/71‑1 (MQ=255) cGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg > 1:545495/1‑70 (MQ=255) ggTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGATTgtggt < 1:524998/71‑1 (MQ=255) ggTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTgtggt < 1:2354202/71‑1 (MQ=255) aaaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat > 1:1949035/1‑71 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgatt > 1:3819499/1‑71 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgatt > 1:2115883/1‑71 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgatt > 1:420430/1‑71 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat < 1:3378043/70‑1 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat < 1:1442459/70‑1 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat < 1:1330575/70‑1 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat < 1:546359/70‑1 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat < 1:387705/70‑1 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtga > 1:3418411/1‑69 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtga > 1:1045201/1‑69 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtga > 1:546438/1‑69 (MQ=255) aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTATGGtgat < 1:1525735/70‑1 (MQ=255) aGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattg < 1:1867554/71‑1 (MQ=255) gCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg > 1:2401362/1‑63 (MQ=255) gCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg > 1:1521483/1‑63 (MQ=255) cTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattga > 1:2870863/1‑68 (MQ=255) cTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattga > 1:3489925/1‑68 (MQ=255) tGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc > 1:422047/1‑71 (MQ=255) tGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc > 1:1171100/1‑71 (MQ=255) ccAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAg > 1:3234246/1‑71 (MQ=255) ccAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAg > 1:3718228/1‑71 (MQ=255) ccAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAg > 1:1618926/1‑71 (MQ=255) cAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGt > 1:683626/1‑71 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGaa > 1:2107497/1‑44 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGaa > 1:816186/1‑44 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGaa > 1:2847802/1‑44 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGaa > 1:1934743/1‑44 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGaa > 1:1572334/1‑44 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGaa > 1:626939/1‑44 (MQ=255) aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc < 1:3552531/71‑1 (MQ=255) ccAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAg < 1:1907105/71‑1 (MQ=255) ccAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAg < 1:1203001/71‑1 (MQ=255) ccAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAg < 1:906549/71‑1 (MQ=255) gCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGa < 1:1894764/63‑1 (MQ=255) gCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGa < 1:1047623/63‑1 (MQ=255) gCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGa < 1:2954120/63‑1 (MQ=255) cGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGa < 1:1456317/62‑1 (MQ=255) tAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGc < 1:1706865/71‑1 (MQ=255) tAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGc < 1:2051741/71‑1 (MQ=255) aGATGTGCTATCGCGCGGCCTGGATGAACTGATTgtggt < 1:3779527/39‑1 (MQ=255) aGATGTGCTATCGCGCGGCCTGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCg > 1:1265778/1‑70 (MQ=255) aGATGTGCTATCGCGCGGCCTGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCg > 1:96628/1‑70 (MQ=255) aGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGc < 1:1537937/71‑1 (MQ=255) aTGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaa > 1:1212987/1‑71 (MQ=255) aTGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaa > 1:2149958/1‑71 (MQ=255) tgtgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaac < 1:2063219/71‑1 (MQ=255) gtgCTATCGCGCGGCCCGGATGAACTGATTgtggt < 1:3147204/35‑1 (MQ=255) gtgCTATCGCGCGGCCCGGATGAACTGATTgtggt < 1:1606754/35‑1 (MQ=255) gtgCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattga > 1:2979985/1‑41 (MQ=255) gtgCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattga > 1:1873057/1‑41 (MQ=255) gtgCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattga > 1:1912867/1‑41 (MQ=255) gtgCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattga > 1:237203/1‑41 (MQ=255) gtgCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattga > 1:2134610/1‑41 (MQ=255) tgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaaca < 1:997294/70‑1 (MQ=255) tgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaaca < 1:3545186/70‑1 (MQ=255) tgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaaca < 1:1089767/70‑1 (MQ=255) tgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaaca < 1:1898663/70‑1 (MQ=255) tgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaaca < 1:2450646/70‑1 (MQ=255) tgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaaca < 1:271028/70‑1 (MQ=255) cTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAAc < 1:1088585/71‑1 (MQ=255) tATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc > 1:1456642/1‑41 (MQ=255) tATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc > 1:3812695/1‑41 (MQ=255) tATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGa < 1:857500/49‑1 (MQ=255) tATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACg > 1:870486/1‑71 (MQ=255) tCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc < 1:2767605/39‑1 (MQ=255) tCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGc > 1:2598238/1‑71 (MQ=255) gcgcgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGcc > 1:1585963/1‑57 (MQ=255) gcgcgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGc < 1:2972030/59‑1 (MQ=255) ggCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAgcgc < 1:1827790/71‑1 (MQ=255) cccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCAc > 1:1550325/1‑71 (MQ=255) cccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCAc < 1:3822649/71‑1 (MQ=255) ccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACt > 1:3438529/1‑71 (MQ=255) ccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACt < 1:1527610/71‑1 (MQ=255) ccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACt < 1:2593983/71‑1 (MQ=255) ccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACt < 1:1487718/71‑1 (MQ=255) ccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACt < 1:3680844/71‑1 (MQ=255) | CAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACT > Exported/40253‑40391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |