| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 936,986 | G→A | 7.0% | G182G (GGG→GGA) | fdnI → | formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 936,986 | 0 | G | A | 7.0% | 333.1 / 9.6 | 100 | G182G (GGG→GGA) | fdnI | formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible |
| Reads supporting (aligned to +/- strand): ref base G (46/47); new base A (3/4); total (49/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 113 total aligned reads displayed.
TCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAG > Exported/936919‑937055 | tcatcCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGgat > 1:3569904/1‑70 (MQ=255)tcatcCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGgat > 1:308322/1‑70 (MQ=255)tcatcCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGgat > 1:1410857/1‑70 (MQ=255)tcatcCTGATCCACGCCATCCTGATCCATATGTATACGGCATTTTGGGTGAAAGGATCGATTAAAGGgat > 1:1309831/1‑70 (MQ=255) catcCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGgatga > 1:451290/1‑71 (MQ=255) catcCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGgatga > 1:2041631/1‑71 (MQ=255) atcCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGgatga < 1:2241527/70‑1 (MQ=255) atcCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGgatga < 1:3582878/70‑1 (MQ=255) atcCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGgatga < 1:3578229/70‑1 (MQ=255) cTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATc < 1:802287/69‑1 (MQ=255) cTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCg < 1:1161715/70‑1 (MQ=255) tGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGgatgatag > 1:2007614/1‑67 (MQ=255) tGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCg > 1:1953999/1‑69 (MQ=255) aTCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaag < 1:508069/70‑1 (MQ=255) aTCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaag < 1:63483/70‑1 (MQ=255) aTCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaa < 1:78520/69‑1 (MQ=255) aTCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCg < 1:2798039/67‑1 (MQ=255) aTCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCg < 1:3715250/67‑1 (MQ=255) tCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATc > 1:3813885/1‑65 (MQ=255) ccACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaaggg > 1:2333870/1‑70 (MQ=255) ccACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaaggg > 1:1624721/1‑70 (MQ=255) gCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGt < 1:3224933/71‑1 (MQ=255) ccTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtc > 1:1801324/1‑71 (MQ=255) cTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaagggaag > 1:3101169/1‑63 (MQ=255) cTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaagggaag > 1:3296586/1‑63 (MQ=255) cTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcg > 1:3719920/1‑71 (MQ=255) cTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcg > 1:596815/1‑71 (MQ=255) tGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcgt > 1:396110/1‑71 (MQ=255) tGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcgt > 1:2343305/1‑71 (MQ=255) aTCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGAATGATCGAAGGGAAGGTAAgtcgtc < 1:2478737/70‑1 (MQ=255) atatGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTgg < 1:1987617/71‑1 (MQ=255) tatGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaagggaagg > 1:264871/1‑56 (MQ=255) tatGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaagggaagg > 1:259153/1‑56 (MQ=255) tatGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaagggaagg > 1:540902/1‑56 (MQ=255) tatGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCg < 1:2696803/47‑1 (MQ=255) tatGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCg < 1:880607/47‑1 (MQ=255) tatGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTgg > 1:65815/1‑70 (MQ=255) tGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGga > 1:3056124/1‑39 (MQ=255) tGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaa > 1:2439455/1‑47 (MQ=255) tGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaa > 1:1510518/1‑47 (MQ=255) gTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATc > 1:1109312/1‑43 (MQ=255) gTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATc > 1:1117952/1‑43 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGtcgtcg > 1:2423899/1‑60 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGtcgtcg > 1:3694834/1‑60 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGtcgtcg > 1:3267345/1‑60 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGtcgtcg > 1:2649459/1‑60 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTTGGCgaaga < 1:509126/71‑1 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCgaaga < 1:1143578/71‑1 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCgaaga < 1:3078518/71‑1 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCgaaga < 1:1768008/71‑1 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCgaaga < 1:3743571/71‑1 (MQ=255) tatGGCATTTTGGGTGAAAGGATCGATCAAAGGGATGATCGAAGGGAAGGTAAGtcgtcg > 1:2562537/1‑60 (MQ=255) atGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATc < 1:2215841/39‑1 (MQ=255) tGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcgtc < 1:2175624/57‑1 (MQ=255) tGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcgtc < 1:2153684/57‑1 (MQ=255) tGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcgtc < 1:364419/57‑1 (MQ=255) tGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcgtc < 1:1036162/57‑1 (MQ=255) tGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcgtc < 1:3782086/57‑1 (MQ=255) tGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcgtc < 1:3808374/57‑1 (MQ=255) tGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtcgtc < 1:3218722/57‑1 (MQ=255) gCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:2027970/1‑57 (MQ=255) gCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:240546/1‑57 (MQ=255) gCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:1545559/1‑57 (MQ=255) gCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAaca < 1:3014678/71‑1 (MQ=255) cATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGt < 1:1177885/46‑1 (MQ=255) cATTTTGGGTGAAAGGATCGATTAAAGGAATGATCGAAGGGAAGGTAAGTCGTCGCTggg > 1:3141920/1‑60 (MQ=255) cATTTTGGGTGAAAGGATCGATTAAAGGAATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:589025/71‑1 (MQ=255) cATTTTGGGTGAAAGGATCGATTAAAGGAATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac > 1:3043993/1‑71 (MQ=255) cATTTTGGGTGAAAGGATCGATTAAAGGAATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:18408/71‑1 (MQ=255) cATTTTGGGTGAAAGGATCGATTAAAGGAATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac > 1:1852223/1‑71 (MQ=255) aTTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac > 1:3445342/1‑70 (MQ=255) aTTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACAcc < 1:1287262/71‑1 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGt < 1:2684592/44‑1 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGt < 1:2181422/44‑1 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGt < 1:2044214/44‑1 (MQ=255) ttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTg > 1:251333/1‑54 (MQ=255) ttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTg > 1:1570736/1‑54 (MQ=255) ttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTg > 1:515895/1‑54 (MQ=255) ttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATc > 1:2711780/1‑71 (MQ=255) gAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCg < 1:1296167/52‑1 (MQ=255) gAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCg < 1:1426461/52‑1 (MQ=255) gAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCg < 1:1469350/52‑1 (MQ=255) gAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCg < 1:1540198/52‑1 (MQ=255) gAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGaaa < 1:482736/58‑1 (MQ=255) gAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGaaa < 1:1974949/58‑1 (MQ=255) aaGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAgt > 1:1871910/1‑38 (MQ=255) aGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTgg > 1:2698872/1‑70 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTa < 1:3158425/71‑1 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTa < 1:1219936/71‑1 (MQ=255) gATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTAt > 1:3803730/1‑71 (MQ=255) gATAGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTAt > 1:844452/1‑71 (MQ=255) aTCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGaaa < 1:533336/52‑1 (MQ=255) gcGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCgaaga < 1:2666881/48‑1 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTGAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaa < 1:2175871/71‑1 (MQ=255) aaaGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAAt > 1:323405/1‑71 (MQ=255) aaGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATc < 1:1046704/71‑1 (MQ=255) aGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATc > 1:2535255/1‑70 (MQ=255) aGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATc > 1:2923763/1‑70 (MQ=255) aGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCg < 1:448454/71‑1 (MQ=255) ggATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCgag < 1:702868/71‑1 (MQ=255) | TCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAG > Exported/936919‑937055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |