Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 937,123 C→T 9.0% intergenic (+29/+378) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported937,1230CT 9.0% 240.1 / 11.6 78intergenic (+29/+378)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (29/42);  new base T (2/5);  total (31/47)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTG  >  Exported/937053‑937188
                                                                      |                                                                 
gagaAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGgc                                                                   >  1:36689/1‑71 (MQ=255)
   aaGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCt                                                                >  1:3467024/1‑71 (MQ=255)
   aaGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCt                                                                >  1:2765874/1‑71 (MQ=255)
    aGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTg                                                               <  1:3480832/71‑1 (MQ=255)
    aGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTg                                                               <  1:1212278/71‑1 (MQ=255)
       cAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTaa                                                            <  1:768163/71‑1 (MQ=255)
       cAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTaa                                                            <  1:2290636/71‑1 (MQ=255)
        aGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTTAACTTTAAGCTGAAAATGGCGCTGTaaa                                                           <  1:3170208/71‑1 (MQ=255)
          aaGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAg                                                         <  1:2168813/71‑1 (MQ=255)
          aaGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAg                                                         <  1:240963/71‑1 (MQ=255)
           aGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAgg                                                        >  1:117664/1‑71 (MQ=255)
           aGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAgg                                                        >  1:2358545/1‑71 (MQ=255)
                aaaaaGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTaaaa                                                          <  1:1554080/64‑1 (MQ=255)
                aaaaaGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTaaaa                                                          <  1:1623128/64‑1 (MQ=255)
                 aaaaGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGTGCTGTAAAAGGCGCCAt                                                  >  1:772994/1‑71 (MQ=255)
                 aaaaGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGt                                                              <  1:2239618/59‑1 (MQ=255)
                 aaaaGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGt                                                              <  1:2253590/59‑1 (MQ=255)
                 aaaaGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCAt                                                  >  1:3563515/1‑71 (MQ=255)
                   aaGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTaa                                                            >  1:1358684/1‑59 (MQ=255)
                    agagagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCAtttt                                               <  1:107989/71‑1 (MQ=255)
                       gagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCat                                            <  1:770093/71‑1 (MQ=255)
                        agTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTaaaa                                                          >  1:1982240/1‑56 (MQ=255)
                        agTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCata                                           <  1:3853701/71‑1 (MQ=255)
                           gaagaaGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGTGCTGTaa                                                            <  1:762897/51‑1 (MQ=255)
                           gaagaaGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGTGCTGTaa                                                            <  1:954364/51‑1 (MQ=255)
                           gaagaaGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGTGCTGTaa                                                            <  1:2750063/51‑1 (MQ=255)
                           gaagaaGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGTGCTGTaa                                                            <  1:2196578/51‑1 (MQ=255)
                           gaagaaGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGTGCTGTaa                                                            <  1:1756489/51‑1 (MQ=255)
                           gaagaaGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCatat                                          <  1:1542242/69‑1 (MQ=255)
                            aagaagGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCa                                             >  1:3595434/1‑65 (MQ=255)
                            aagaagGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTg                                        <  1:1970545/70‑1 (MQ=255)
                             agaagGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTg                                        >  1:1221832/1‑69 (MQ=255)
                              gaagGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTaaaa                                                          >  1:933501/1‑50 (MQ=255)
                                agGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGa                                    >  1:1861240/1‑70 (MQ=255)
                                 gggATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTaaaa                                                          >  1:618000/1‑47 (MQ=255)
                                    atatAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACg                               <  1:3680917/71‑1 (MQ=255)
                                    atatAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACg                               <  1:3607400/71‑1 (MQ=255)
                                       taATCTCTTTTGAACTTTAAGCTGAAAATGGTGCTGTaaaa                                                          >  1:773584/1‑41 (MQ=255)
                                       taATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTaaa                                                           <  1:1688037/40‑1 (MQ=255)
                                       taATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCAtttt                                               <  1:587797/52‑1 (MQ=255)
                                       taATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTg                                        <  1:1871105/59‑1 (MQ=255)
                                       taATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTg                                        <  1:1680801/59‑1 (MQ=255)
                                       taATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAAc                                <  1:3366243/67‑1 (MQ=255)
                                         aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTa                                                             <  1:457979/36‑1 (MQ=255)
                                         aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTa                                                             <  1:2710096/36‑1 (MQ=255)
                                         aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTa                                                             <  1:983754/36‑1 (MQ=255)
                                         aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTa                                                             <  1:1210483/36‑1 (MQ=255)
                                         aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTa                                                             <  1:1379427/36‑1 (MQ=255)
                                         aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAg                                                         >  1:1875675/1‑40 (MQ=255)
                                         aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAg                                                         >  1:2253246/1‑40 (MQ=255)
                                          tctcTTTTGAACTTTAAGCTGAAAATGGCGCTGTaa                                                            <  1:3530412/36‑1 (MQ=255)
                                          tctcTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCAttt                                                <  1:1905605/48‑1 (MQ=255)
                                          tctcTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCAttt                                                <  1:3661393/48‑1 (MQ=255)
                                          tctcTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCt                         >  1:302802/1‑71 (MQ=255)
                                          tctcTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCACCAttt                                                <  1:1690136/48‑1 (MQ=255)
                                           ctctTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCtt                        >  1:2012514/1‑71 (MQ=255)
                                           ctctTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCtt                        >  1:2966517/1‑71 (MQ=255)
                                              ttttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCttt                       <  1:2682270/69‑1 (MQ=255)
                                              ttttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCttt                       <  1:3323941/69‑1 (MQ=255)
                                              ttttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGt                     >  1:311422/1‑71 (MQ=255)
                                               tttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCtt                        <  1:1159656/67‑1 (MQ=255)
                                                ttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCa                                             >  1:2316768/1‑45 (MQ=255)
                                                ttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCa                                             >  1:3472749/1‑45 (MQ=255)
                                                   aaCTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATg                <  1:1878528/71‑1 (MQ=255)
                                                     cTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGcc              >  1:803590/1‑71 (MQ=255)
                                                      tttAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCg             >  1:3150826/1‑71 (MQ=255)
                                                        tAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGtt                    <  1:1632964/62‑1 (MQ=255)
                                                        tAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGtt                    <  1:175868/62‑1 (MQ=255)
                                                        tAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCgg            <  1:2119205/70‑1 (MQ=255)
                                                        tAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCg             <  1:1859830/69‑1 (MQ=255)
                                                         aaGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGa           <  1:2981758/70‑1 (MQ=255)
                                                            cTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATg                >  1:1699443/1‑62 (MQ=255)
                                                             tGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcgg      >  1:256032/1‑71 (MQ=39)
                                                                 aaTGGCGCTGTAAAAGGCGCCATTTTCATATTTTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTg  <  1:477360/71‑1 (MQ=21)
                                                                 aaTGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGt   >  1:497830/1‑70 (MQ=35)
                                                                   tGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGc               >  1:736220/1‑56 (MQ=35)
                                                                   tGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGc               >  1:643194/1‑56 (MQ=35)
                                                                   tGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGc               >  1:810000/1‑56 (MQ=35)
                                                                   tGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGc               >  1:1045550/1‑56 (MQ=35)
                                                                     gcgcTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCg             <  1:658648/56‑1 (MQ=35)
                                                                      cgcTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCtt                        <  1:2931425/44‑1 (MQ=38)
                                                                      cgcTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCtt                        <  1:3795738/44‑1 (MQ=38)
                                                                      cgcTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCtt                        <  1:1053693/44‑1 (MQ=38)
                                                                      |                                                                 
GAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTG  >  Exported/937053‑937188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: