Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	204,511	C→T	100%	intergenic (+111/‑73)	alaV → / → rrlH	tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	204,511	0	C	T	100.0%	20.1 / NA	10	intergenic (+111/‑73)	alaV/rrlH	tRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGG  >  Exported/204491‑204577                     |                                                                   aaaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  <  1:3525645/71‑1 (MQ=9)  aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  >  1:1513005/1‑70 (MQ=255)  aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  >  1:2377978/1‑70 (MQ=255)  aaaTTGAAACACTGAACAATGAAAGTTGTTAGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  >  1:3399747/1‑70 (MQ=11)   aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  >  1:2559007/1‑69 (MQ=255)    aTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg               <  1:830214/71‑1 (MQ=255)      tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACAATGATGGATCGCAAGa              >  1:1154188/1‑70 (MQ=11)      tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACAATGATGGATCGCAAGa              >  1:474914/1‑70 (MQ=11)        aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc           <  1:1573698/71‑1 (MQ=255)        aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc           <  1:2388208/71‑1 (MQ=255)                 acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg  >  1:2661531/1‑71 (MQ=255)                     |                                                                   AAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGG  >  Exported/204491‑204577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑