Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported204,5560AG100.0% 179.5 / NA 60intergenic (+156/‑28)alaV/rrlHtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/36);  total (24/36)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCC  >  Exported/204491‑204615
                                                                 |                                                           
aaaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                        <  1:3525645/71‑1 (MQ=9)
 aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                        >  1:2377978/1‑70 (MQ=255)
 aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                        >  1:1513005/1‑70 (MQ=255)
 aaaTTGAAACACTGAACAATGAAAGTTGTTAGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                        >  1:3399747/1‑70 (MQ=11)
  aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                        >  1:2559007/1‑69 (MQ=255)
   aTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg                                                     <  1:830214/71‑1 (MQ=255)
     tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACAATGATGGATCGCAAGa                                                    >  1:1154188/1‑70 (MQ=11)
     tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACAATGATGGATCGCAAGa                                                    >  1:474914/1‑70 (MQ=11)
       aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                 <  1:1573698/71‑1 (MQ=255)
       aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                 <  1:2388208/71‑1 (MQ=255)
                acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg                                        >  1:2661531/1‑71 (MQ=255)
                  aatgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGtt                                      >  1:496456/4‑71 (MQ=255)
                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                   <  1:518236/70‑1 (MQ=25)
                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                   <  1:522755/70‑1 (MQ=25)
                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                   <  1:641211/70‑1 (MQ=25)
                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                   <  1:2036090/70‑1 (MQ=25)
                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                   <  1:242515/70‑1 (MQ=25)
                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                    >  1:706399/1‑69 (MQ=25)
                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                    >  1:2903679/1‑69 (MQ=25)
                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                    >  1:2350174/1‑69 (MQ=25)
                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                    >  1:2114044/1‑69 (MQ=25)
                       aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                 <  1:1676177/71‑1 (MQ=21)
                         gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt                               <  1:3535066/71‑1 (MQ=21)
                         gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt                               <  1:129386/71‑1 (MQ=21)
                          ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                              <  1:3021757/71‑1 (MQ=21)
                          ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                              <  1:2923201/71‑1 (MQ=21)
                          ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                              <  1:2405637/71‑1 (MQ=21)
                             ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTaa                            <  1:397455/70‑1 (MQ=25)
                             ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTaa                            <  1:2456361/70‑1 (MQ=25)
                             ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTaa                            <  1:1889187/70‑1 (MQ=25)
                               cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                          <  1:423517/70‑1 (MQ=25)
                                   aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                          <  1:271438/66‑1 (MQ=25)
                                   aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                          <  1:193871/66‑1 (MQ=25)
                                   aGTCTCTCAAATTATCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                          <  1:3417010/66‑1 (MQ=255)
                                       tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt                                             <  1:3597665/43‑1 (MQ=255)
                                       tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                 >  1:3289093/1‑71 (MQ=21)
                                       tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                 >  1:1025224/1‑71 (MQ=21)
                                       tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                 >  1:1521171/1‑71 (MQ=21)
                                       tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                 >  1:2678990/1‑71 (MQ=21)
                                         tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc                                           >  1:2094655/1‑43 (MQ=255)
                                         tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                          <  1:3232962/60‑1 (MQ=255)
                                         tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                          <  1:415154/60‑1 (MQ=255)
                                         tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa               >  1:3353556/1‑71 (MQ=21)
                                         tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa               >  1:3108020/1‑71 (MQ=21)
                                         tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa               >  1:2449973/1‑71 (MQ=21)
                                         tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa               >  1:1765710/1‑71 (MQ=21)
                                          cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                    <  1:3151826/49‑1 (MQ=255)
                                          cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                    <  1:2011332/49‑1 (MQ=255)
                                          cAAATTTTCGCAACACGATGATGGAGCGCAAGAAACATCTTCGGGTtgt                                    <  1:3691968/49‑1 (MQ=11)
                                           aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca             >  1:886591/1‑71 (MQ=21)
                                           aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca             >  1:2920991/1‑71 (MQ=21)
                                           aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa               <  1:357838/69‑1 (MQ=25)
                                           aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa               <  1:684205/69‑1 (MQ=25)
                                            aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                          <  1:274366/57‑1 (MQ=255)
                                               tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg                                <  1:2347874/48‑1 (MQ=255)
                                                 tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg        <  1:613427/70‑1 (MQ=25)
                                                   gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                    <  1:137514/40‑1 (MQ=255)
                                                    cAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                          <  1:3501509/49‑1 (MQ=255)
                                                      acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc  >  1:3514638/1‑71 (MQ=21)
                                                       cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc   <  1:2691012/69‑1 (MQ=25)
                                                             gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg        <  1:1318928/58‑1 (MQ=255)
                                                             gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg        <  1:2125284/58‑1 (MQ=255)
                                                                 |                                                           
AAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCC  >  Exported/204491‑204615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: