| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,108,696 | A→T | 5.1% | D156V (GAT→GTT) | kbaY → | tagatose 6‑phosphate aldolase 1, kbaY subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,108,696 | 0 | A | T | 5.1% | 391.7 / 8.7 | 133 | D156V (GAT→GTT) | kbaY | tagatose 6‑phosphate aldolase 1, kbaY subunit |
| Reads supporting (aligned to +/- strand): ref base A (74/52); new base T (2/5); total (76/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 133 total aligned reads displayed.
AGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGC > Exported/2108628‑2108765 | aGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATc > 1:1464354/1‑71 (MQ=255)aGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATc > 1:3151467/1‑71 (MQ=255)aGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATc > 1:1475180/1‑71 (MQ=255) aaCTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCca > 1:1425941/1‑71 (MQ=255) cTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCcac > 1:498349/1‑70 (MQ=255) cTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCcac > 1:2342021/1‑70 (MQ=255) gggCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCcac > 1:3022178/1‑68 (MQ=255) ggCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACaag < 1:422942/70‑1 (MQ=255) cgccTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGc < 1:2202386/71‑1 (MQ=255) gccTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCt < 1:2370013/71‑1 (MQ=255) ccTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTa < 1:126962/71‑1 (MQ=255) ccTGGGCGGTGTTGAAGATGACATGAGCGTTGACACCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTa < 1:276352/71‑1 (MQ=255) tGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTaaa > 1:3381321/1‑71 (MQ=255) cGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCt < 1:2644783/71‑1 (MQ=255) cGGTGTTGAAGATGACATGAGCGTTGACGCAGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCt < 1:3835943/71‑1 (MQ=255) gtgtTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTa < 1:1800960/63‑1 (MQ=255) ttGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACaagaag < 1:2980878/57‑1 (MQ=255) ttGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACaagaag < 1:832154/57‑1 (MQ=255) ttGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTc > 1:478981/1‑71 (MQ=255) ttGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTc > 1:1091843/1‑71 (MQ=255) ttGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACAGATCCACaagaag < 1:164647/57‑1 (MQ=255) gAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCcac > 1:2721510/1‑49 (MQ=255) gAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCcac > 1:3445910/1‑49 (MQ=255) gAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCcac > 1:2420830/1‑49 (MQ=255) gAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCcac > 1:2360974/1‑49 (MQ=255) gAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACaaga > 1:3073601/1‑53 (MQ=255) gAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACaaga > 1:2654037/1‑53 (MQ=255) gAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACaaga > 1:1220386/1‑53 (MQ=255) gAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCg < 1:1048502/70‑1 (MQ=255) aaGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGaa > 1:1745750/1‑71 (MQ=255) aaGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGa < 1:1027145/70‑1 (MQ=255) aGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAac > 1:1564676/1‑71 (MQ=255) gATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCGACAAGAAGCTAAACGCTTTGTCGAact > 1:182663/1‑71 (MQ=255) gATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAact < 1:1493678/71‑1 (MQ=255) gATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAact > 1:3392035/1‑71 (MQ=255) aTGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGa < 1:1309772/67‑1 (MQ=255) gACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAactgac > 1:3760342/1‑71 (MQ=255) aCATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAactgac < 1:3047177/70‑1 (MQ=255) cATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTa < 1:3172052/50‑1 (MQ=255) cATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAactgactg < 1:2062649/71‑1 (MQ=255) attaGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTgg < 1:1305000/68‑1 (MQ=255) aTGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCttt < 1:3714726/57‑1 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTaaa > 1:2037462/1‑50 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTaaa > 1:3215327/1‑50 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTaaa > 1:1921561/1‑50 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTaaa > 1:1647706/1‑50 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGc > 1:1802147/1‑71 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGc > 1:1368375/1‑71 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGc > 1:255235/1‑71 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGc > 1:1699899/1‑71 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGc > 1:874266/1‑71 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCA‑AAGAAGCTaaa > 1:1986749/1‑49 (MQ=255) tGAGCGTTGACGCCGAAAGTGCATTCCTCACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGc > 1:1753967/1‑71 (MQ=255) gAGCGTTGACGCCGAAAGTGCATTCCTGACCGTTCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCg > 1:2618926/1‑71 (MQ=255) gAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGt > 1:1469873/1‑57 (MQ=255) gAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCg > 1:3733607/1‑71 (MQ=255) aGCGTTGACGCCGAAAGTGCATTCCTGACCGATCca > 1:3270299/1‑36 (MQ=255) aGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGt > 1:2487878/1‑71 (MQ=255) aGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGt > 1:1837439/1‑71 (MQ=255) aGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGt > 1:2483500/1‑71 (MQ=255) aGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGt > 1:184195/1‑71 (MQ=255) gCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTc < 1:2945606/71‑1 (MQ=255) cGTTGACGCCGAAAGTGCATTCCTGCCCGATCCACAAGAAGCTAAACGCTTTGTCGAac > 1:2414495/1‑59 (MQ=255) cGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCt < 1:2589858/43‑1 (MQ=255) cGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCt < 1:2218978/43‑1 (MQ=255) cGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCt < 1:1158443/43‑1 (MQ=255) gTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGa < 1:125131/71‑1 (MQ=255) cGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGcc < 1:2271049/71‑1 (MQ=255) cGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGcc < 1:2978135/71‑1 (MQ=255) ccGAAAGTGCATTCCTGACCGATCCACAAGAAGCTa < 1:3261403/36‑1 (MQ=255) cGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTgg < 1:2798976/71‑1 (MQ=255) aaaGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCg > 1:2538724/1‑71 (MQ=255) aaGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGctg < 1:1504471/71‑3 (MQ=255) aaGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCgg < 1:3767911/71‑1 (MQ=255) aaGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCgg < 1:3761958/71‑1 (MQ=255) aGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCgg > 1:850038/1‑70 (MQ=255) aGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCgg > 1:771654/1‑70 (MQ=255) gTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGt > 1:3163824/1‑40 (MQ=255) gTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGt > 1:1098432/1‑40 (MQ=255) gTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCgg > 1:3589088/1‑69 (MQ=255) cATTCCTGACCGTTCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGc < 1:2322641/70‑1 (MQ=255) cATTCCTGACCGTTCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGc < 1:39453/70‑1 (MQ=255) cATTCCTGACCGTTCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGc < 1:3706654/70‑1 (MQ=255) ttCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGcc > 1:3429246/1‑58 (MQ=255) ttCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGcc > 1:1488599/1‑58 (MQ=255) ttCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTg < 1:2380103/60‑1 (MQ=255) ttCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGAt < 1:903159/71‑1 (MQ=255) tCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGAtt > 1:1305304/1‑71 (MQ=255) ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACATCCTGGCGGTAGCGATTg > 1:1808733/1‑71 (MQ=255) ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCg > 1:3388492/1‑67 (MQ=255) ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCg > 1:1388749/1‑67 (MQ=255) ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCg > 1:714484/1‑67 (MQ=255) ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTg > 1:2489360/1‑71 (MQ=255) cTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTgg < 1:1159795/71‑1 (MQ=255) cTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTgg < 1:1155348/71‑1 (MQ=255) cTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTgg < 1:509665/71‑1 (MQ=255) cTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTgg > 1:2044485/1‑71 (MQ=255) ccGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACg < 1:2422029/71‑1 (MQ=255) cGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACgg > 1:2193656/1‑71 (MQ=255) gATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGgc > 1:1879538/1‑71 (MQ=255) | AGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGC > Exported/2108628‑2108765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |