Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,948,260 G→A 7.8% intergenic (‑20/+118) yjjB ← / ← yjjP conserved inner membrane protein/predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,948,2600GA 7.8% 461.4 / 20.5 141intergenic (‑20/+118)yjjB/yjjPconserved inner membrane protein/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (71/59);  new base A (9/2);  total (80/61)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 145 total aligned reads displayed.

CATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAG  >  Exported/2948193‑2948329
                                                                   |                                                                     
cATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGc                                                                    >  1:1723253/1‑71 (MQ=255)
 aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTAGGGAACCATGACCTATCGAGc                                                                    >  1:3063859/1‑70 (MQ=255)
    aaGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                >  1:2665768/1‑71 (MQ=255)
    aaGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                >  1:1444623/1‑71 (MQ=255)
         tGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           >  1:3203502/1‑71 (MQ=255)
         tGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           >  1:539213/1‑71 (MQ=255)
          gACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcagc                                                            >  1:3195393/1‑69 (MQ=255)
          gACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           >  1:1151845/1‑70 (MQ=255)
          gACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           <  1:3501629/70‑1 (MQ=255)
          gACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           <  1:1327832/70‑1 (MQ=255)
          gACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           >  1:174551/1‑70 (MQ=255)
             cACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGcaca                                                       >  1:3699604/1‑71 (MQ=255)
               cTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcag                                                             <  1:3315539/63‑1 (MQ=255)
               cTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcag                                                             <  1:1379901/63‑1 (MQ=255)
               cTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcag                                                             <  1:3030625/63‑1 (MQ=255)
                 cAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcag                                                             <  1:726843/61‑1 (MQ=255)
                 cAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAg                                                   >  1:1959638/1‑71 (MQ=255)
                  aaTATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGc                                                  <  1:1412671/71‑1 (MQ=255)
                  aaTATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGc                                                  >  1:1039572/1‑71 (MQ=255)
                  aaTATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGc                                                  >  1:2662849/1‑71 (MQ=255)
                  aaTATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGc                                                  >  1:1552414/1‑71 (MQ=255)
                    tatTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg                                                 >  1:2016853/1‑70 (MQ=255)
                      ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                              >  1:2550119/1‑71 (MQ=255)
                      ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                              >  1:227438/1‑71 (MQ=255)
                      ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                              >  1:3459651/1‑71 (MQ=255)
                      ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa                                               <  1:620011/70‑1 (MQ=255)
                      ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa                                               <  1:2753819/70‑1 (MQ=255)
                       tCACCCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa                                               <  1:1537210/69‑1 (MQ=255)
                        cAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcag                                                             >  1:2381853/1‑54 (MQ=255)
                         aaCCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGc                                           <  1:1715264/71‑1 (MQ=255)
                           cccGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGccc                                         >  1:3568918/1‑71 (MQ=255)
                           cccGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGccc                                         >  1:2892605/1‑71 (MQ=255)
                           cccGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGcc                                          >  1:3604842/1‑70 (MQ=255)
                            ccGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCg                                        <  1:671284/71‑1 (MQ=255)
                            ccGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCg                                        <  1:2363812/71‑1 (MQ=255)
                               cTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAg                                                                     >  1:3741693/1‑39 (MQ=255)
                               cTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAg                                                                     >  1:2020866/1‑39 (MQ=255)
                               cTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAg                                                                     >  1:3842307/1‑39 (MQ=255)
                               cTGGTCATCAAGATCATTCGGGAACCATGAACTATCGAg                                                                     >  1:3204872/1‑39 (MQ=255)
                                   tcatcaAGATCATTCGGGAACCATGACCTATCGAGCCAagc                                                               <  1:2193961/41‑1 (MQ=255)
                                    catcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGaa                                >  1:2719045/1‑71 (MQ=255)
                                    catcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGaa                                >  1:2383473/1‑71 (MQ=255)
                                     atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGcac                                                        <  1:757589/46‑1 (MQ=255)
                                     atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGcac                                                        <  1:3430475/46‑1 (MQ=255)
                                     atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGcac                                                        <  1:1490874/46‑1 (MQ=255)
                                     atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGaa                                <  1:2457373/70‑1 (MQ=255)
                                      tcaAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTa                                               <  1:1395107/54‑1 (MQ=255)
                                      tcaAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTa                                               <  1:3636946/54‑1 (MQ=255)
                                       caAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGcaca                                                       <  1:2566009/45‑1 (MQ=255)
                                       caAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGcaca                                                       <  1:577399/45‑1 (MQ=255)
                                        aaGATCATTCTGGAACCATGACCTATCGAGCCAag                                                                <  1:588400/35‑1 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCCAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt                            >  1:1758230/1‑71 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                <  1:905169/35‑1 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                <  1:3175415/35‑1 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                <  1:1838652/35‑1 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                <  1:15226/35‑1 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                <  1:2871733/35‑1 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           >  1:763878/1‑40 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCAc                                     <  1:1048531/62‑1 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt                            >  1:3463963/1‑71 (MQ=255)
                                        aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGt                             >  1:1101363/1‑70 (MQ=255)
                                         tgATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           <  1:1968595/38‑1 (MQ=255)
                                         aGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           <  1:2699504/39‑1 (MQ=255)
                                         aGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           <  1:1610923/39‑1 (MQ=255)
                                         aGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                                           <  1:2637117/39‑1 (MQ=255)
                                         aGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt                            >  1:1442015/1‑70 (MQ=255)
                                         aGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTg                           <  1:1176391/71‑1 (MQ=255)
                                         aGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTg                           <  1:2460481/71‑1 (MQ=255)
                                           aTCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGca                                                         >  1:2175666/1‑39 (MQ=255)
                                           aTCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGca                                                         >  1:103329/1‑39 (MQ=255)
                                           aTCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGca                                                         >  1:1590336/1‑39 (MQ=255)
                                           aTCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGca                                                         >  1:230142/1‑39 (MQ=255)
                                             cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGcaca                                                       <  1:319486/39‑1 (MQ=255)
                                             cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACg                              >  1:684055/1‑64 (MQ=255)
                                             cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAaca                       >  1:3065495/1‑71 (MQ=255)
                                               ttCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac                      >  1:374106/1‑70 (MQ=255)
                                               ttCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac                      >  1:2786004/1‑70 (MQ=255)
                                                 cGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCa                    <  1:783116/70‑1 (MQ=255)
                                                 cGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCa                    <  1:742602/70‑1 (MQ=255)
                                                  gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCa                    <  1:831510/69‑1 (MQ=255)
                                                  gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATc                  >  1:181855/1‑71 (MQ=255)
                                                  gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATc                  >  1:407450/1‑71 (MQ=255)
                                                      aCCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACggg                                  <  1:1277668/51‑1 (MQ=255)
                                                      aCCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCaa              <  1:3228618/71‑1 (MQ=255)
                                                      aCCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCaa              <  1:3521557/71‑1 (MQ=255)
                                                      aCCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCa               >  1:2445218/1‑70 (MQ=255)
                                                         aTGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGc           >  1:3858309/1‑71 (MQ=255)
                                                         aTGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGc           >  1:109170/1‑71 (MQ=255)
                                                          tGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGcc          >  1:2180622/1‑71 (MQ=255)
                                                          tGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGcc          >  1:902496/1‑71 (MQ=255)
                                                          tGACCTATCAAGCCAAGCAGCGCAGACCAGCGTAACGCCCGCACGGGAACGTTGaa                         >  1:803957/1‑56 (MQ=255)
                                                          tGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGaa                         >  1:1844771/1‑56 (MQ=255)
                                                            aCCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCg         <  1:2199413/70‑1 (MQ=255)
                                                               tATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAAc                               >  1:1664964/1‑45 (MQ=255)
                                                               tATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCg     >  1:2950642/1‑71 (MQ=255)
                                                               tATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCg     >  1:729319/1‑71 (MQ=255)
                                                                 tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCg     >  1:1813423/1‑69 (MQ=255)
                                                                 tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCg     <  1:1967404/69‑1 (MQ=255)
                                                                   gAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAg  >  1:2423807/1‑70 (MQ=255)
                                                                   gAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAg  >  1:827067/1‑70 (MQ=255)
                                                                   |                                                                     
CATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAG  >  Exported/2948193‑2948329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: