Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 2,982,587 | T→C | 11.8% | G97G (GGT→GGC) | creB → | DNA‑binding response regulator in two‑component regulatory system with CreC |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 2,982,587 | 0 | T | C | 11.8% | 134.1 / 10.4 | 51 | G97G (GGT→GGC) | creB | DNA‑binding response regulator in two‑component regulatory system with CreC |
Reads supporting (aligned to +/- strand): ref base T (23/22); new base C (3/3); total (26/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 |
CGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTAC > Exported/2982517‑2982651 | cGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGt > 1:2898647/1‑71 (MQ=255) cGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGt < 1:2048723/71‑1 (MQ=255) ggCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTg > 1:1165829/1‑71 (MQ=255) cGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCt < 1:2129594/71‑1 (MQ=255) cGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCt < 1:293632/71‑1 (MQ=255) aCCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgac < 1:3837497/70‑1 (MQ=255) gTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTa > 1:533287/1‑71 (MQ=255) gTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTa > 1:394565/1‑71 (MQ=255) cTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGt > 1:580358/1‑71 (MQ=255) ccTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCt > 1:2158314/1‑70 (MQ=255) cTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTa < 1:1618463/70‑1 (MQ=255) tGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTaaa > 1:1618438/1‑71 (MQ=255) aCGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAAcc > 1:1353644/1‑71 (MQ=255) aCGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAAcc > 1:3190401/1‑71 (MQ=255) cGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCg < 1:3675534/71‑1 (MQ=255) cGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCg < 1:3293980/71‑1 (MQ=255) cGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCg < 1:762847/71‑1 (MQ=255) ccGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGtttt < 1:3583020/71‑1 (MQ=255) ccGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGtttt < 1:899790/71‑1 (MQ=255) ccGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGtttt < 1:1206369/71‑1 (MQ=255) cGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTc < 1:1414884/71‑1 (MQ=255) gAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCa > 1:2839562/1‑71 (MQ=255) gAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCa > 1:1596080/1‑71 (MQ=255) gAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCa > 1:3594626/1‑71 (MQ=255) aaGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCcgc > 1:3118424/1‑71 (MQ=255) aaGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCcgc < 1:3198395/71‑1 (MQ=255) gagGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCcgcg > 1:3387739/1‑70 (MQ=255) gTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCg < 1:1830333/54‑1 (MQ=255) cGCCTGCTTGGGCTGGAAATTGGTGATGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTgcgc > 1:328130/1‑71 (MQ=255) gCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAggg > 1:2887198/1‑71 (MQ=255) gCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAggg > 1:209167/1‑71 (MQ=255) cTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAggg > 1:1033054/1‑70 (MQ=255) cTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAggg > 1:182160/1‑70 (MQ=255) cTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGt < 1:707747/71‑1 (MQ=255) tGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAgtg < 1:2341993/53‑1 (MQ=255) aaTTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCa > 1:300652/1‑65 (MQ=255) aaTTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCCCCt > 1:2911913/1‑68 (MQ=255) aaTTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTAc > 1:1190828/1‑71 (MQ=255) aTTGGCGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAgtg < 1:393676/48‑1 (MQ=38) aTTGGCGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAgtg < 1:3751695/48‑1 (MQ=38) aTTGGCGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAgtg < 1:3723703/48‑1 (MQ=38) ttGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGaa < 1:557895/44‑1 (MQ=255) ttGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGaa < 1:613414/44‑1 (MQ=255) ttGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGaa < 1:863050/44‑1 (MQ=255) tGGCGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGtgtg > 1:433756/1‑48 (MQ=38) tGGCGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGtgtg > 1:1267850/1‑48 (MQ=38) tGGCGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGtgtg > 1:334513/1‑48 (MQ=38) ggTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAg > 1:3819829/1‑43 (MQ=255) ggTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAg > 1:2833020/1‑43 (MQ=255) gTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTa < 1:1342427/65‑1 (MQ=255) gTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTa < 1:668286/65‑1 (MQ=255) gTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTa < 1:2965863/65‑1 (MQ=255) gTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTa < 1:3416482/65‑1 (MQ=255) | CGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTAC > Exported/2982517‑2982651 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |