Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 323,666 C→G 7.4% S26S (TCC→TCG htpG → molecular chaperone HSP90 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported323,6660CG 7.4% 205.6 / 5.6 67S26S (TCC→TCGhtpGmolecular chaperone HSP90 family
Reads supporting (aligned to +/- strand):  ref base C (33/29);  new base G (3/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCC  >  Exported/323597‑323733
                                                                     |                                                                   
aCAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTcc                                                                     >  1:387031/1‑70 (MQ=255)
  aaGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCaa                                                                   >  1:2949882/1‑70 (MQ=255)
  aaGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCaa                                                                   >  1:2600398/1‑70 (MQ=255)
  aaGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCaa                                                                   >  1:925145/1‑70 (MQ=255)
  aaGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCaa                                                                   >  1:3326708/1‑70 (MQ=255)
  aaGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCaa                                                                   >  1:1329571/1‑70 (MQ=255)
     aaaCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATa                                                                 >  1:1760882/1‑69 (MQ=255)
     aaaCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATa                                                                 >  1:2144113/1‑69 (MQ=255)
     aaaCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATa                                                                 >  1:672075/1‑69 (MQ=255)
      aaCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATaaa                                                               <  1:1804515/70‑1 (MQ=255)
              gTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTc                                                      <  1:1233004/71‑1 (MQ=255)
                tttCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAAt                                                          >  1:610590/1‑65 (MQ=255)
                tttCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCt                                                    >  1:371222/1‑71 (MQ=255)
                   cagtcagAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGc                                                  <  1:1369884/70‑1 (MQ=255)
                     gtcagAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATc                                                         <  1:3238967/61‑1 (MQ=255)
                      tcagAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGa                                              <  1:3861034/71‑1 (MQ=255)
                      tcagAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGa                                              <  1:816421/71‑1 (MQ=255)
                        agAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGc                                            >  1:2990543/1‑71 (MQ=255)
                          aaGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCtt                                          <  1:2270439/71‑1 (MQ=255)
                          aaGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCt                                           >  1:1903994/1‑70 (MQ=255)
                           aGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTa                                         <  1:3550891/71‑1 (MQ=255)
                           aGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATACCAATAAAGAAATCTTCCTGCGTGAGCTTa                                         <  1:32092/71‑1 (MQ=255)
                            gTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGaaa                                                           <  1:999422/52‑1 (MQ=255)
                            gTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTg                                               <  1:2645261/64‑1 (MQ=255)
                               aaaCAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAtctc                                     >  1:3263640/1‑71 (MQ=255)
                                aaCAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                    <  1:2622752/71‑1 (MQ=255)
                                 aCAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTa                                   <  1:3053736/71‑1 (MQ=255)
                                  cAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGaa                                                            <  1:3326170/45‑1 (MQ=255)
                                  cAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGaa                                                            <  1:1192059/45‑1 (MQ=255)
                                  cAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGaa                                                            <  1:2806911/45‑1 (MQ=255)
                                   aGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAAc                                 >  1:2346884/1‑71 (MQ=255)
                                      ttCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGcc                              >  1:3372737/1‑71 (MQ=255)
                                       tCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCt                             >  1:3089595/1‑71 (MQ=255)
                                        cTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAAt                                                          >  1:3427458/1‑41 (MQ=255)
                                        cTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTc                            >  1:2425837/1‑71 (MQ=255)
                                        cTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTc                            >  1:2029569/1‑71 (MQ=255)
                                          gCACCTGATGCTCCATTCTCTCTATTCCAATAAAGAAATCTTcc                                                     >  1:1367647/1‑44 (MQ=255)
                                          gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTcc                                                     >  1:1323488/1‑44 (MQ=255)
                                          gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTcc                                                     >  1:380800/1‑44 (MQ=255)
                                          gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                    >  1:2331874/1‑61 (MQ=255)
                                          gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                    >  1:2294259/1‑61 (MQ=255)
                                          gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                    >  1:3042005/1‑61 (MQ=255)
                                           cACCTGATGATTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGAtttcc                                    <  1:578528/60‑2 (MQ=255)
                                           cACCTGATGATTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGAtttcc                                    <  1:352644/60‑2 (MQ=255)
                                              cTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCg                                                 <  1:831061/44‑1 (MQ=255)
                                               tgatgaTCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                    <  1:2318751/56‑1 (MQ=255)
                                               tgatgaTCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTcc                           <  1:329765/65‑1 (MQ=255)
                                                gatgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                    <  1:3105474/55‑1 (MQ=255)
                                                 atgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcggc                   >  1:182401/1‑71 (MQ=255)
                                                 atgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcggc                   >  1:1450887/1‑71 (MQ=255)
                                                 atgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcg                     <  1:559793/69‑1 (MQ=255)
                                                  tgatTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGAcgccgcc                  >  1:1492779/1‑70 (MQ=255)
                                                  tgatTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGAcgccgcc                  >  1:3132299/1‑70 (MQ=255)
                                                  tgatTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGAcgccgcc                  >  1:1239532/1‑70 (MQ=255)
                                                  tgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAt                                        >  1:1109411/1‑49 (MQ=255)
                                                  tgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAt                                        >  1:2667968/1‑49 (MQ=255)
                                                    atCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGcggcgg                 <  1:789668/70‑1 (MQ=255)
                                                     tCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGAc               <  1:3252897/71‑1 (MQ=255)
                                                     tCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGAc               <  1:2694386/71‑1 (MQ=255)
                                                       cATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACa              <  1:2168675/70‑1 (MQ=255)
                                                         ttCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGc           <  1:2886575/71‑1 (MQ=255)
                                                           ctctctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAt                                        >  1:2573957/1‑40 (MQ=255)
                                                              tctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGt      >  1:3369438/1‑71 (MQ=255)
                                                              tctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGt      >  1:3346866/1‑71 (MQ=255)
                                                               ctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGtt     >  1:300829/1‑71 (MQ=255)
                                                                 aTTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa                <  1:778748/58‑1 (MQ=255)
                                                                  ttCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa                <  1:1535752/57‑1 (MQ=255)
                                                                  ttCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTcc  <  1:754836/71‑1 (MQ=255)
                                                                    ccAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCg       >  1:706672/1‑64 (MQ=255)
                                                                     |                                                                   
ACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCC  >  Exported/323597‑323733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: