BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,359,227	Δ1 bp	6.2%	noncoding (1868/2905 nt)	rrlA ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,359,227	0	G	.	6.2%	441.4 / 17.4	128	noncoding (1868/2905 nt)	rrlA	23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base G (52/68); new base . (4/4); total (56/72)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 129 total aligned reads displayed.

TCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCGTGTTTG  >  Exported/2359158‑2359296

tCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCg                                                                       >  1:4492345/1‑70 (MQ=40)

  gCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGc                                                                     >  1:1422678/1‑70 (MQ=255)

  gCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCt                                                                    <  1:4026561/71‑1 (MQ=255)

    tACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGACTTGCGGCTaa                                                                  >  1:2760510/1‑71 (MQ=255)

      ccTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAAcc                                                                <  1:1358160/71‑1 (MQ=255)

      ccTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAAc                                                                 <  1:481719/70‑1 (MQ=255)

      ccTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAAc                                                                 <  1:3001165/70‑1 (MQ=255)

        ttAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAAccc                                                               >  1:270459/1‑70 (MQ=255)

          aGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAAcccc                                                              <  1:704621/69‑1 (MQ=255)

          aGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCAt                                                            >  1:2239166/1‑71 (MQ=255)

          aGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCAt                                                            >  1:1473903/1‑71 (MQ=255)

          aGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCAt                                                            >  1:3899569/1‑71 (MQ=255)

            gACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATc                                                           >  1:4533983/1‑70 (MQ=255)

             aCCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCa                                                          <  1:2130759/70‑1 (MQ=255)

                gTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTTCGGCTAACCCCATCAATTa                                                      <  1:2331442/71‑1 (MQ=255)

                gTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTa                                                      <  1:135732/71‑1 (MQ=255)

                gTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTa                                                      <  1:304538/71‑1 (MQ=255)

                gTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTa                                                      <  1:2020650/71‑1 (MQ=255)

                   ataGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAAcc                                                   >  1:2519374/1‑71 (MQ=255)

                   ataGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAAcc                                                   >  1:4664257/1‑71 (MQ=255)

                   ataGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAAcc                                                   >  1:2794448/1‑71 (MQ=255)

                   ataGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAAc                                                    >  1:3397363/1‑70 (MQ=255)

                   ataGTTA‑GGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCt                                                  >  1:4053513/1‑71 (MQ=255)

                      gTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTc                                                <  1:2105836/71‑1 (MQ=255)

                       ttACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAAc                                                    >  1:1581698/1‑66 (MQ=255)

                        tACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCg                                              >  1:2973215/1‑71 (MQ=255)

                         aCGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCgg                                             >  1:1817641/1‑71 (MQ=255)

                         aCGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCgg                                             >  1:1628993/1‑71 (MQ=255)

                         aCGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCg                                              <  1:4122058/70‑1 (MQ=255)

                                ccgTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCggg                                      <  1:2599286/71‑1 (MQ=255)

                                ccgTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCggg                                      <  1:2081539/71‑1 (MQ=255)

                                ccgTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCggg                                      >  1:4306036/1‑71 (MQ=255)

                                ccgTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCggg                                      >  1:4119937/1‑71 (MQ=255)

                                 cgTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCgg                                             >  1:853218/1‑63 (MQ=255)

                                 cgTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCggg                                      >  1:1581385/1‑70 (MQ=255)

                                  gTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCa                                    <  1:479897/71‑1 (MQ=255)

                                   tttACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAg                                   <  1:3517394/71‑1 (MQ=255)

                                    ttACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGTTAACCCCATCAATTAACCTTCCgg                                             >  1:259497/1‑60 (MQ=255)

                                    ttACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAAcc                                                   >  1:2620233/1‑54 (MQ=255)

                                    ttACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCgg                                             >  1:3893748/1‑60 (MQ=255)

                                    ttACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAg                                   >  1:2462933/1‑70 (MQ=255)

                                     tACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGc                                 >  1:4574777/1‑71 (MQ=255)

                                     tACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGc                                 >  1:2578230/1‑71 (MQ=255)

                                     tACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGc                                 >  1:4663004/1‑71 (MQ=255)

                                     tACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGc                                 >  1:2123853/1‑71 (MQ=255)

                                     tACCGGGGCTTCGATCAAGAGCTTCGCCTTGC‑GCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCg                                >  1:255552/1‑71 (MQ=9)

                                     tACCGGGGCTTCGATCAAGAGCTTCGCCTTGC‑GCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCg                                >  1:2504858/1‑71 (MQ=9)

                                     tACCGGGGCTTCGATCAAGAGCTTCGCCTTGC‑GCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCg                                >  1:434069/1‑71 (MQ=9)

                                      aCCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGc                                 <  1:1191977/70‑1 (MQ=255)

                                       ccGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGt                               <  1:1036893/71‑1 (MQ=255)

                                        cGGGGCTTCGATCAAGAGCTTCGCCTTGC‑GCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTca                             <  1:4660593/71‑1 (MQ=9)

                                        cGGGGCTTCGATCAAGAGCTTCGCCTTGC‑GCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTca                             <  1:3674017/71‑1 (MQ=9)

                                        cGGGGCTTCGATCAAGAGCTTCGCCTTGC‑GCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTca                             <  1:906748/71‑1 (MQ=9)

                                          gggCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTca                             >  1:1605247/1‑70 (MQ=255)

                                           ggCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCTGGCAGGCGTcaca                           <  1:1123440/71‑1 (MQ=255)

                                               tCGATCAAGAGCTTCGCCTTCCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCg                        <  1:4341842/70‑1 (MQ=255)

                                                cGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCg                        <  1:1166777/69‑1 (MQ=255)

                                                 gATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGtat                     <  1:702824/71‑1 (MQ=255)

                                                 gATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGtat                     <  1:1911181/71‑1 (MQ=255)

                                                 gATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGta                      <  1:1272871/70‑1 (MQ=255)

                                                 gATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGta                      <  1:2945635/70‑1 (MQ=255)

                                                  aTCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCa                                    <  1:334748/55‑1 (MQ=255)

                                                  aTCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCa                                    <  1:1262603/55‑1 (MQ=255)

                                                  aTCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCa                                    <  1:295375/55‑1 (MQ=255)

                                                  aTCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCg                                <  1:965534/59‑1 (MQ=255)

                                                  aTCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGtata                    >  1:593878/1‑71 (MQ=255)

                                                   tCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACATCGTATAc                   >  1:727474/1‑71 (MQ=255)

                                                   tCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATAc                   <  1:3651786/71‑1 (MQ=255)

                                                   tCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATAc                   <  1:3534495/71‑1 (MQ=255)

                                                    cAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTca                             >  1:2256572/1‑60 (MQ=255)

                                                    cAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTc                              >  1:3657062/1‑59 (MQ=255)

                                                    cAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTc                              >  1:1653631/1‑59 (MQ=255)

                                                    cAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACg                  >  1:4067497/1‑71 (MQ=255)

                                                    cAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACg                  >  1:3376952/1‑71 (MQ=255)

                                                      agagCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCa                                    <  1:451784/51‑1 (MQ=255)

                                                      agagCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCa                                    <  1:1761545/51‑1 (MQ=255)

                                                      agagCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCa                                    <  1:247362/51‑1 (MQ=255)

                                                      agagCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCa                                    <  1:3063302/51‑1 (MQ=255)

                                                      agagCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTc                >  1:3545788/1‑71 (MQ=255)

                                                        agCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCa              >  1:55617/1‑71 (MQ=255)

                                                          cTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCAc                                          <  1:571517/41‑1 (MQ=255)

                                                             ctccTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTca                             <  1:2227169/49‑1 (MQ=255)

                                                             cGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACtt           <  1:4227322/69‑1 (MQ=255)

                                                              gCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTc         <  1:3886037/70‑1 (MQ=255)

                                                               ccTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGt                       >  1:3248957/1‑55 (MQ=255)

                                                               ccTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGt                       >  1:1366270/1‑55 (MQ=255)

                                                                cTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCgtg      <  1:128866/71‑1 (MQ=39)

                                                                cTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCgtg      <  1:2171255/71‑1 (MQ=39)

                                                                cTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCgtg      <  1:4736097/71‑1 (MQ=39)

                                                                cTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCgtg      <  1:3318206/71‑1 (MQ=39)

                                                                  tGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACtt           <  1:671996/64‑1 (MQ=37)

                                                                  tGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACtt           <  1:2550491/64‑1 (MQ=37)

                                                                  tGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACtt           <  1:872670/64‑1 (MQ=37)

                                                                   gCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCgtgt     <  1:683139/69‑1 (MQ=35)

                                                                   gCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCgtgt     <  1:4616610/69‑1 (MQ=35)

                                                                   gCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCg        >  1:1466634/1‑66 (MQ=35)

                                                                   gCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCg        >  1:368029/1‑66 (MQ=35)

                                                                   gCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCGTGttt   >  1:724909/1‑71 (MQ=34)

                                                                   gCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCGTGtt    <  1:47948/70‑1 (MQ=35)

                                                                    cGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCGTGTTTg  <  1:1395446/71‑1 (MQ=32)

TCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCCTTGCGGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCGTGTTTG  >  Exported/2359158‑2359296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`