| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 13,094 | A→T | 6.0% | D311V (GAT→GTT) | dnaK → | chaperone Hsp70, co‑chaperone with DnaJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 13,094 | 0 | A | T | 6.0% | 350.2 / 11.3 | 116 | D311V (GAT→GTT) | dnaK | chaperone Hsp70, co‑chaperone with DnaJ |
| Reads supporting (aligned to +/- strand): ref base A (57/52); new base T (2/5); total (59/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
AGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATA > Exported/13026‑13162 | aGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATc < 1:4448626/71‑1 (MQ=255)aGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATc < 1:4267125/71‑1 (MQ=255) aCGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTg > 1:2144313/1‑71 (MQ=255) gcgcCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTgg > 1:2241487/1‑70 (MQ=255) gcgACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTgg > 1:2203250/1‑70 (MQ=255) gcgACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGt < 1:3483243/71‑1 (MQ=255) cgACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTa < 1:3845711/71‑1 (MQ=255) cgACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTa < 1:133884/71‑1 (MQ=255) gACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTa > 1:2421242/1‑70 (MQ=255) gACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTa > 1:583082/1‑70 (MQ=255) aCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTgg < 1:1049951/67‑1 (MQ=255) aCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTgg < 1:3502090/67‑1 (MQ=255) aCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTgg < 1:492637/67‑1 (MQ=255) aCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTgg < 1:2263037/67‑1 (MQ=255) ccGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAAc > 1:2074010/1‑71 (MQ=255) gTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTa < 1:1644273/65‑1 (MQ=255) gTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTa < 1:643781/65‑1 (MQ=255) cGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAATCCGTTc < 1:2507/70‑1 (MQ=255) cGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTc > 1:3487703/1‑70 (MQ=255) cGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTc > 1:3206858/1‑70 (MQ=255) gAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCa < 1:814323/71‑1 (MQ=255) gAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCa < 1:797436/71‑1 (MQ=255) gAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCa < 1:1851424/71‑1 (MQ=255) aaaCACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCAt > 1:2117096/1‑71 (MQ=255) cacaTGAACATCAAAGTGACTCGTGCGAAACTGGAAATCCTGGTTGAAGATCTGGTAAACCGTTCCATTGa < 1:1366589/71‑1 (MQ=255) cacaTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGa < 1:4090861/71‑1 (MQ=255) acaTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAg > 1:260077/1‑71 (MQ=255) acaTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGATGAAGATCTGGTAAACCGTTCCAtt > 1:1079169/1‑68 (MQ=255) aTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGcc > 1:170863/1‑71 (MQ=255) aTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAgac > 1:265372/1‑69 (MQ=255) aTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGcc > 1:3022014/1‑71 (MQ=255) aTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGcc > 1:2366090/1‑71 (MQ=255) aTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGcc > 1:4343047/1‑71 (MQ=255) tGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATc > 1:1059451/1‑48 (MQ=255) tGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCg < 1:1820335/71‑1 (MQ=255) aaCATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCg > 1:2159752/1‑56 (MQ=255) aaCATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCg > 1:2795490/1‑56 (MQ=255) aaCATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCt > 1:3670958/1‑71 (MQ=255) cATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGa < 1:4252695/71‑1 (MQ=255) tCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGa > 1:2323032/1‑69 (MQ=255) aaaGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAg < 1:4277473/70‑1 (MQ=255) aaaGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGAt > 1:2923333/1‑39 (MQ=255) aaaGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGAt > 1:4495376/1‑39 (MQ=255) aaaGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGAt > 1:3859431/1‑39 (MQ=255) aGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTg < 1:809710/71‑1 (MQ=255) aGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTg < 1:4055167/71‑1 (MQ=255) gTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAACCCGTTCCATTGAGcc > 1:1263335/1‑59 (MQ=39) gTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCTTTCCATTGAGCCGCTGAAAGTTGc < 1:2199231/71‑1 (MQ=255) gTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGc < 1:4079360/71‑1 (MQ=255) tCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTa < 1:3475077/37‑1 (MQ=255) tCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTcc < 1:561221/46‑1 (MQ=255) tCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTcc < 1:4406821/46‑1 (MQ=255) tCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTcc < 1:3400997/46‑1 (MQ=255) tCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTcc < 1:2422156/46‑1 (MQ=255) tCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGTCGCTGAAAGTTGCACTg > 1:1980745/1‑70 (MQ=255) tgTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTcc < 1:1502183/44‑1 (MQ=255) gTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCAt > 1:3141879/1‑46 (MQ=255) gTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCAt > 1:2171302/1‑46 (MQ=255) gTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAg > 1:4756282/1‑71 (MQ=255) gTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAg < 1:4197294/71‑1 (MQ=255) gCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAAcc > 1:3943665/1‑37 (MQ=255) gCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAAc > 1:4169915/1‑36 (MQ=255) cGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCAc > 1:4563755/1‑63 (MQ=255) cGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGAc > 1:3928165/1‑71 (MQ=255) aaaCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGc > 1:3782355/1‑71 (MQ=255) aaaCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGc > 1:18805/1‑71 (MQ=255) aaaCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGc > 1:1404425/1‑71 (MQ=255) aaCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAg < 1:4303812/54‑1 (MQ=255) aaCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAg < 1:2089476/54‑1 (MQ=255) aaCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAg < 1:3218977/54‑1 (MQ=255) aCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGaa < 1:1395279/51‑1 (MQ=255) aCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTg > 1:3545239/1‑71 (MQ=255) aCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTg < 1:1297390/71‑1 (MQ=255) aCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTg < 1:2403651/71‑1 (MQ=255) tGGATAGGCTGGTTGAAGATCTGGTAAACCGTTCCATTg < 1:3740775/39‑1 (MQ=38) ggAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGaa < 1:1873245/48‑1 (MQ=255) cTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGt < 1:2977002/71‑1 (MQ=255) tGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGt < 1:1597373/43‑1 (MQ=255) tGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGc < 1:1234529/62‑1 (MQ=255) ggTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGc < 1:3633033/61‑1 (MQ=255) ttGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCt < 1:1509787/71‑1 (MQ=255) ttGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCt < 1:194199/71‑1 (MQ=255) gAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTgg < 1:710956/56‑1 (MQ=255) gAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTgg < 1:1763164/56‑1 (MQ=255) gAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTgg < 1:3683023/56‑1 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAg > 1:82147/1‑37 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAg > 1:4587017/1‑37 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAg > 1:2990361/1‑37 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAg > 1:1124607/1‑37 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAg > 1:1047075/1‑37 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGa > 1:2350381/1‑50 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGa > 1:1944273/1‑50 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGa > 1:1682471/1‑50 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGa > 1:1206555/1‑50 (MQ=255) gAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGa > 1:1007848/1‑71 (MQ=255) aGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGata > 1:3195799/1‑71 (MQ=255) aGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGata > 1:2570861/1‑71 (MQ=255) aTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGt < 1:2576025/61‑1 (MQ=255) aTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGa < 1:3624585/67‑1 (MQ=255) aTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGa < 1:2255361/67‑1 (MQ=255) | AGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATA > Exported/13026‑13162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |