| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,197,287 | G→A | 7.5% | R421R (CGG→CGA) | degQ → | serine endoprotease, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,197,287 | 0 | G | A | 7.5% | 385.7 / 15.4 | 120 | R421R (CGG→CGA) | degQ | serine endoprotease, periplasmic |
| Reads supporting (aligned to +/- strand): ref base G (74/37); new base A (3/6); total (77/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
CAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAA > Exported/2197218‑2197351 | cAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCggg > 1:4351188/1‑71 (MQ=255) aaaGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGt < 1:2544436/71‑1 (MQ=255) aaaGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGt < 1:1309202/71‑1 (MQ=255) aGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTg > 1:2262163/1‑70 (MQ=255) aGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCAATCGAGtt > 1:1723931/1‑69 (MQ=255) gAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAAc > 1:2417863/1‑71 (MQ=255) aGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGaa < 1:1214431/68‑1 (MQ=255) aGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc > 1:1633437/1‑71 (MQ=255) aGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc > 1:4008119/1‑71 (MQ=255) aGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc > 1:2677058/1‑71 (MQ=255) gCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCg < 1:1281757/71‑1 (MQ=255) cccAGCTGCTCAGGCTGTCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCTCGATCGGGTGAACTc < 1:2621896/69‑1 (MQ=255) cccAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACt > 1:3139710/1‑68 (MQ=255) cccAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACt > 1:873860/1‑68 (MQ=255) cccAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACt > 1:3575007/1‑68 (MQ=255) cccAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACt > 1:949032/1‑68 (MQ=255) cccAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACt > 1:4555545/1‑68 (MQ=255) cccAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc < 1:130084/69‑1 (MQ=255) cccAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc < 1:836400/69‑1 (MQ=255) ccAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa < 1:731395/70‑1 (MQ=255) gctgctCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGc > 1:2199082/1‑71 (MQ=255) gctgctCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGc > 1:399904/1‑71 (MQ=255) gctgctCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGc > 1:4708494/1‑71 (MQ=255) gctgctCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGc > 1:285772/1‑71 (MQ=255) gctgctCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGc > 1:1007753/1‑71 (MQ=255) gctCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTg > 1:106464/1‑70 (MQ=255) ggctggctTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa > 1:522266/1‑59 (MQ=255) ggctggctTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa > 1:4097010/1‑59 (MQ=255) tggctTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGt > 1:1827609/1‑70 (MQ=255) tggctTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGt > 1:146236/1‑70 (MQ=255) gctTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTa > 1:3025641/1‑69 (MQ=255) cAAAAAGACGATGTGATCATTGGCGTCAACCGCAATCGAGt < 1:2622244/41‑1 (MQ=255) cAAAAAGACGATGTGATCATTGGCGTCAACCGCAATCGAGt < 1:3074498/41‑1 (MQ=255) cAAAAAGACGATGTGATCATTGGCGTCAACCGCAATCGAGt < 1:2053287/41‑1 (MQ=255) cAAAAAGACGATGTGATCATTGGCGTCAACCGCAATCGAGt < 1:4293419/41‑1 (MQ=255) aaaaaGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTg < 1:4758394/71‑1 (MQ=255) aaaaaGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATCGCTGAAATGCGTa < 1:3922349/63‑1 (MQ=255) aaaaaGACGATGTGATCATTGGCGTCAACCGCAATCGAGt < 1:3884757/40‑1 (MQ=255) aaaGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:1621444/1‑63 (MQ=255) aaaGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:3738393/1‑63 (MQ=255) aaaGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:1844940/1‑63 (MQ=255) aaaGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggc < 1:4614624/71‑1 (MQ=255) aaaGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggc > 1:4688562/1‑71 (MQ=255) aaaGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggc > 1:1528502/1‑71 (MQ=255) aaGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcg > 1:4180075/1‑71 (MQ=255) gACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAtt > 1:3643351/1‑45 (MQ=255) gACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcgg > 1:3594395/1‑70 (MQ=255) cGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaa < 1:2500264/71‑1 (MQ=255) cGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaa < 1:2878843/71‑1 (MQ=255) cGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCa > 1:3474255/1‑70 (MQ=255) cGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCa > 1:82442/1‑70 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc < 1:4149739/38‑1 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc < 1:1073768/38‑1 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:3821256/1‑57 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:3364358/1‑57 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:953301/1‑57 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:3020210/1‑57 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:1385221/1‑57 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:1880845/1‑57 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:3025574/1‑57 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaaa < 1:1237370/71‑1 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaa > 1:1983881/1‑70 (MQ=255) gATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaa > 1:1233728/1‑70 (MQ=255) gtgATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTg < 1:3792604/60‑1 (MQ=255) gtgATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaa < 1:2376989/67‑1 (MQ=255) gtgATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAcc > 1:3372401/1‑71 (MQ=255) aTCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTa < 1:4159054/49‑1 (MQ=255) aTCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGc > 1:1944778/1‑71 (MQ=255) ttGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaa < 1:365199/46‑1 (MQ=255) ttGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaa < 1:1790934/46‑1 (MQ=255) ttGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaa < 1:1776611/46‑1 (MQ=255) ggCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAg > 1:1899771/1‑46 (MQ=255) ggCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAg > 1:1220470/1‑46 (MQ=255) ggCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAg > 1:1008171/1‑46 (MQ=255) ggCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAg > 1:2724760/1‑46 (MQ=255) ggCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCcatcat > 1:549494/1‑71 (MQ=255) ggCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCcatca < 1:3753970/70‑1 (MQ=255) ggCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCcatca < 1:1654515/70‑1 (MQ=255) gCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTa < 1:2170593/42‑1 (MQ=255) gCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTa < 1:4247965/42‑1 (MQ=255) gCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCt > 1:440553/1‑49 (MQ=255) gCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCt > 1:2821123/1‑49 (MQ=255) gCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCt > 1:1426099/1‑49 (MQ=255) gTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:2852398/1‑42 (MQ=255) gTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa > 1:1757832/1‑42 (MQ=255) gTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGc > 1:528209/1‑71 (MQ=255) tCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGcc < 1:224296/71‑1 (MQ=255) aaCCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCt > 1:3191171/1‑71 (MQ=255) aaCCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCt > 1:936946/1‑71 (MQ=255) aaCCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCt > 1:2931701/1‑71 (MQ=255) aaCCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCt > 1:3697363/1‑71 (MQ=255) ccGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTg > 1:1988591/1‑70 (MQ=255) cgcgATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaaaa > 1:212557/1‑51 (MQ=255) cgcgATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCg > 1:1484014/1‑64 (MQ=255) cgATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCaaa < 1:1417274/71‑1 (MQ=255) cgATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCaaa < 1:68304/71‑1 (MQ=255) cgATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCaaa > 1:2806958/1‑71 (MQ=255) cgATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCaaa < 1:1024477/71‑1 (MQ=255) gggTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCa < 1:540601/64‑1 (MQ=255) gggTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCa < 1:3199587/64‑1 (MQ=255) | CAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAA > Exported/2197218‑2197351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |