Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,465,020 T→G 8.0% A226A (GCT→GCG atpB → F0 sector of membrane‑bound ATP synthase, subunit a

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,465,0200TG 8.0% 298.3 / 21.8 113A226A (GCT→GCGatpBF0 sector of membrane‑bound ATP synthase, subunit a
Reads supporting (aligned to +/- strand):  ref base T (54/50);  new base G (4/5);  total (58/55)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 114 total aligned reads displayed.

AACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGATCA  >  Exported/2464950‑2465087
                                                                      |                                                                   
aaCCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCt                                                                     >  1:1807739/1‑71 (MQ=255)
     gTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCt                                                                >  1:2906340/1‑71 (MQ=255)
       ttCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGt                                                              >  1:3826078/1‑71 (MQ=255)
         cACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGtt                                                             <  1:2248924/70‑1 (MQ=255)
         cACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGtt                                                             <  1:4451963/70‑1 (MQ=255)
            tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                                         >  1:2635796/1‑71 (MQ=255)
            tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                                         >  1:514956/1‑71 (MQ=255)
              ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                                         <  1:2739454/69‑1 (MQ=255)
               gTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCgtgg                                                      <  1:2549788/71‑1 (MQ=255)
                cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                                     >  1:3412248/2‑71 (MQ=255)
                cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                                     >  1:74925/2‑71 (MQ=255)
                cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                                     >  1:3859644/2‑71 (MQ=255)
                    cGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTggtggt                                                  <  1:696204/70‑1 (MQ=255)
                      aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTTGTCTGTTGCCGTGGTGGTcac                                               <  1:3958726/71‑1 (MQ=255)
                      aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                               <  1:1115985/71‑1 (MQ=255)
                      aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                               <  1:4201384/71‑1 (MQ=255)
                          ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                                            <  1:64827/70‑1 (MQ=255)
                          ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                                            <  1:2488532/70‑1 (MQ=255)
                          ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                                            <  1:4328085/70‑1 (MQ=255)
                          ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                                            <  1:2154619/70‑1 (MQ=255)
                          ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGATACCGTGGTGGTCACAGt                                            <  1:1632785/70‑1 (MQ=255)
                            cGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                                          <  1:15451/70‑1 (MQ=255)
                             ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                                        <  1:997892/71‑1 (MQ=255)
                             ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                                        >  1:2217735/1‑71 (MQ=255)
                             ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                                        >  1:16380/1‑71 (MQ=255)
                             ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                                        <  1:4606629/71‑1 (MQ=255)
                              gTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGa                                         <  1:1384777/69‑1 (MQ=255)
                                aaCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAg                                             >  1:1712296/1‑63 (MQ=255)
                                 aCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                                        <  1:2764699/67‑1 (MQ=255)
                                 aCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                                        <  1:743229/67‑1 (MQ=255)
                                  cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                                    >  1:3447607/1‑70 (MQ=255)
                                  cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                                    >  1:3863304/1‑70 (MQ=255)
                                  cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                                    >  1:3078560/1‑70 (MQ=255)
                                  cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa                                   <  1:2361874/71‑1 (MQ=255)
                                  cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa                                   <  1:1962365/71‑1 (MQ=255)
                                  cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa                                   <  1:3911839/71‑1 (MQ=255)
                                    tGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                                          >  1:2334127/1‑62 (MQ=255)
                                    tGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                                          >  1:4203597/1‑62 (MQ=255)
                                      tATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                               >  1:2402960/1‑71 (MQ=255)
                                      tATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                               <  1:1362587/71‑1 (MQ=255)
                                        tGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGc                             >  1:1662452/1‑71 (MQ=255)
                                          ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGcc                            >  1:2085340/1‑70 (MQ=255)
                                          ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                           >  1:3548770/1‑71 (MQ=255)
                                          ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                           <  1:1048145/71‑1 (MQ=255)
                                          ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                           <  1:1042453/71‑1 (MQ=255)
                                            ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg                         >  1:2799251/1‑71 (MQ=255)
                                            ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg                         >  1:2321249/1‑71 (MQ=255)
                                            ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGGCACAGTGGATCCTGAATGTGCCGTg                         <  1:4360089/71‑1 (MQ=255)
                                             gTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg                         <  1:3695612/70‑1 (MQ=255)
                                                aGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGc                      <  1:3216128/70‑1 (MQ=255)
                                                  cTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAt                   <  1:520638/71‑1 (MQ=255)
                                                  cTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAt                   <  1:4398708/71‑1 (MQ=255)
                                                  cTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAt                   <  1:4393422/71‑1 (MQ=255)
                                                    gATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAttt                 >  1:972411/1‑71 (MQ=255)
                                                    gATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAttt                 >  1:3552012/1‑71 (MQ=255)
                                                      ttttTATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcaca                                              >  1:3003804/1‑40 (MQ=255)
                                                       tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTggg                       >  1:3011947/1‑62 (MQ=255)
                                                       tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTggg                       >  1:3163585/1‑62 (MQ=255)
                                                       tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtttt                >  1:3544829/1‑69 (MQ=255)
                                                       tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTc               >  1:3592156/1‑70 (MQ=255)
                                                       tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTc               >  1:446305/1‑70 (MQ=255)
                                                       tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTc               >  1:1905292/1‑70 (MQ=255)
                                                        ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAt                                 <  1:295231/51‑1 (MQ=255)
                                                        ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtttt                >  1:622970/1‑68 (MQ=255)
                                                        ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTcc              >  1:1887765/1‑70 (MQ=255)
                                                        ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCca             >  1:1683319/1‑71 (MQ=255)
                                                        ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCca             >  1:2287400/1‑71 (MQ=255)
                                                          cATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                               >  1:2752542/1‑51 (MQ=255)
                                                          cATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                               >  1:994907/1‑51 (MQ=255)
                                                          cATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                               >  1:2768369/1‑51 (MQ=255)
                                                           aTTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCcaca           <  1:3039782/70‑1 (MQ=255)
                                                              cTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtttt                >  1:1340670/1‑62 (MQ=255)
                                                              cTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtttt                >  1:1531955/1‑62 (MQ=255)
                                                              cTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtttt                >  1:3923819/1‑62 (MQ=255)
                                                               tGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCt       >  1:50942/1‑70 (MQ=255)
                                                                 aTTGCTGGTCTGTTGCCGTGGTTGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTg      <  1:459564/69‑1 (MQ=255)
                                                                 aTTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCt       <  1:1817719/68‑1 (MQ=255)
                                                                 aTTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTg      <  1:3555850/69‑1 (MQ=255)
                                                                 aTTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTg      <  1:530313/69‑1 (MQ=255)
                                                                 aTTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTg      <  1:3038737/69‑1 (MQ=255)
                                                                 aTTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGa     <  1:2027988/70‑1 (MQ=255)
                                                                  ttGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgtg                              <  1:1681309/44‑1 (MQ=255)
                                                                  ttGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGAt    >  1:1990087/1‑70 (MQ=255)
                                                                  ttGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGATc   <  1:4405918/71‑1 (MQ=255)
                                                                   tGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                           >  1:3144707/1‑46 (MQ=255)
                                                                   tGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                           >  1:3265570/1‑46 (MQ=255)
                                                                   tGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                           >  1:635763/1‑46 (MQ=255)
                                                                   tGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                           >  1:4458876/1‑46 (MQ=255)
                                                                   tGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                           >  1:3176016/1‑46 (MQ=255)
                                                                    gCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGcc                            >  1:2290259/1‑44 (MQ=255)
                                                                    gCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGcc                            >  1:857090/1‑44 (MQ=255)
                                                                    gCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGATCa  <  1:17348/70‑1 (MQ=255)
                                                                    gCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGATCa  <  1:186183/70‑1 (MQ=255)
                                                                    gCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGATCa  <  1:2530053/70‑1 (MQ=255)
                                                                    gCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGATCa  <  1:2596142/70‑1 (MQ=255)
                                                                    gCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGATCa  <  1:3245912/70‑1 (MQ=255)
                                                                     cTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtt                  <  1:4520059/53‑1 (MQ=255)
                                                                     cTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtt                  <  1:4101893/53‑1 (MQ=255)
                                                                     cTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtt                  <  1:3476680/53‑1 (MQ=255)
                                                                     cTGGTCTGTTGCCGTAGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtt                  <  1:4687412/53‑1 (MQ=255)
                                                                      |                                                                   
AACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACATCCTGATCA  >  Exported/2464950‑2465087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: