Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,469,985 A→T 5.2% S323C (AGC→TGC) ‡ atpD → F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,469,9850AT 5.2% 348.4 / 8.3 115S323C (AGC→TGC) ‡atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (59/50);  new base T (3/3);  total (62/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 116 total aligned reads displayed.

CGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGA  >  Exported/2469920‑2470055
                                                                 |                                                                      
cGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                                   >  1:909841/1‑71 (MQ=255)
  gATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                                   >  1:1188678/1‑69 (MQ=255)
  gATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCa                                                                 <  1:3408123/71‑1 (MQ=255)
   aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACTCAACCGTGGTACTGAGCCGTCAg                                                                <  1:165944/71‑1 (MQ=255)
   aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                                   >  1:4318262/1‑68 (MQ=255)
   aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                <  1:2933339/71‑1 (MQ=255)
   aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                <  1:4146390/71‑1 (MQ=255)
   aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                <  1:3786272/71‑1 (MQ=255)
   aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                <  1:4550302/71‑1 (MQ=255)
   aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                <  1:132014/71‑1 (MQ=255)
    tgacttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                <  1:3659978/70‑1 (MQ=255)
     gacttggctGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                                   >  1:607228/1‑66 (MQ=255)
        ttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                                           >  1:3997982/1‑71 (MQ=255)
               acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                     <  1:85322/70‑1 (MQ=255)
               acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                     <  1:1752367/70‑1 (MQ=255)
               acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                     <  1:2921162/70‑1 (MQ=255)
               acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                    >  1:3232864/1‑71 (MQ=255)
               acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                    >  1:2792014/1‑71 (MQ=255)
                  cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                                 <  1:2862459/71‑1 (MQ=255)
                  cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                                 <  1:3589691/71‑1 (MQ=255)
                  cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                                 <  1:908250/71‑1 (MQ=255)
                   gTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                                <  1:1819097/71‑1 (MQ=255)
                      tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                                               >  1:3825130/1‑69 (MQ=255)
                      tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATc                                              >  1:4016342/1‑70 (MQ=255)
                      tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATc                                              >  1:4189031/1‑70 (MQ=255)
                        cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGAt                                                              >  1:2130285/1‑52 (MQ=255)
                        cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                                           >  1:4562971/1‑55 (MQ=255)
                        cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                                           >  1:376330/1‑55 (MQ=255)
                        cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                            <  1:3438039/70‑1 (MQ=255)
                        cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                            <  1:150178/70‑1 (MQ=255)
                        cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                            <  1:2598282/70‑1 (MQ=255)
                        cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                            <  1:869574/70‑1 (MQ=255)
                         ggCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                            <  1:244764/69‑1 (MQ=255)
                          gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                    <  1:4256067/60‑1 (MQ=255)
                          gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                    <  1:3428217/60‑1 (MQ=255)
                          gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                                         <  1:4720782/71‑1 (MQ=255)
                          gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                                         <  1:105299/71‑1 (MQ=255)
                           cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                                        <  1:4189578/71‑1 (MQ=255)
                           cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                                        <  1:3161384/71‑1 (MQ=255)
                            aaCCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGCTCGCGTCTCTGGGTATCTACCCgg                                       >  1:2563542/1‑71 (MQ=255)
                            aaCCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCgg                                       >  1:4063829/1‑71 (MQ=255)
                               caccTTTGCGCACCTTGACGGAACCGTGGTACTGAGCCGTCAGATcg                                                            >  1:206391/1‑47 (MQ=255)
                               caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                                    >  1:3415904/1‑69 (MQ=255)
                               caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                                    >  1:2501833/1‑69 (MQ=255)
                               caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                                    >  1:4025323/1‑69 (MQ=255)
                                accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCg                                                                    >  1:3440109/1‑38 (MQ=255)
                                accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                                   >  1:1557602/1‑39 (MQ=255)
                                accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                                               >  1:2342622/1‑59 (MQ=255)
                                accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCTGGCCGt                                   <  1:1108/71‑1 (MQ=255)
                                accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGt                                   <  1:4736167/71‑1 (MQ=255)
                                 ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                                  <  1:2324941/71‑1 (MQ=255)
                                 ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                                  >  1:1017555/1‑71 (MQ=255)
                                 ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                                  <  1:3585632/71‑1 (MQ=255)
                                 ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTATGGGTATCTACCCGGCCGtt                                  <  1:1707410/71‑1 (MQ=255)
                                  cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                            >  1:3475372/1‑60 (MQ=255)
                                  cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAc                                           >  1:426069/1‑61 (MQ=255)
                                  cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAc                                           >  1:2893327/1‑61 (MQ=255)
                                  cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTg                                 <  1:2410584/71‑1 (MQ=255)
                                    ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                                         >  1:1676958/1‑45 (MQ=255)
                                    ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                                         >  1:511006/1‑45 (MQ=255)
                                    ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                                         >  1:25921/1‑45 (MQ=255)
                                    ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAc                               >  1:2247333/1‑71 (MQ=255)
                                     tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCt                                             >  1:1432523/1‑56 (MQ=255)
                                     tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                              >  1:2835828/1‑71 (MQ=255)
                                     tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                              >  1:3236522/1‑71 (MQ=255)
                                      gcgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                              <  1:728405/70‑1 (MQ=255)
                                       cgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAccc                             <  1:3554573/70‑1 (MQ=255)
                                        ccACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                           <  1:759270/70‑1 (MQ=255)
                                        ccACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                           <  1:3967805/70‑1 (MQ=255)
                                         cACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                           <  1:83372/70‑1 (MQ=255)
                                          aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                     <  1:3237960/43‑1 (MQ=255)
                                          aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                         <  1:2977417/71‑1 (MQ=255)
                                          aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                         >  1:360219/1‑71 (MQ=255)
                                           ccTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                          >  1:1975031/1‑69 (MQ=255)
                                             ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                            >  1:1802068/1‑65 (MQ=255)
                                             ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                            >  1:1244383/1‑65 (MQ=255)
                                             ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                            >  1:253635/1‑65 (MQ=255)
                                             ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                            >  1:684147/1‑65 (MQ=255)
                                              tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                                >  1:2251103/1‑44 (MQ=255)
                                              tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt                     >  1:1984961/1‑71 (MQ=255)
                                              tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt                     >  1:3280172/1‑71 (MQ=255)
                                                aCGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTcc                   <  1:4084956/71‑1 (MQ=255)
                                                 cGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                         <  1:1570183/64‑1 (MQ=255)
                                                   cAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTccac                 >  1:395314/1‑70 (MQ=255)
                                                    aaCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTccac                 >  1:1038244/1‑69 (MQ=255)
                                                     aCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAg              <  1:3378230/71‑1 (MQ=255)
                                                     aCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAg              <  1:2985224/71‑1 (MQ=255)
                                                      ccGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGc             >  1:1132770/1‑71 (MQ=255)
                                                         tGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt          >  1:3083266/1‑71 (MQ=255)
                                                           gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc         <  1:4330114/70‑1 (MQ=255)
                                                           gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc         <  1:3932433/70‑1 (MQ=255)
                                                           gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc         <  1:2988030/70‑1 (MQ=255)
                                                           gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc         <  1:2704942/70‑1 (MQ=255)
                                                            tACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAg       <  1:3365512/71‑1 (MQ=255)
                                                               tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCTGCCGTTGACCCGCt                          >  1:3114619/1‑49 (MQ=255)
                                                               tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                          >  1:1269333/1‑49 (MQ=255)
                                                               tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                          >  1:3048460/1‑49 (MQ=255)
                                                               tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                          >  1:2733773/1‑49 (MQ=255)
                                                               tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                          >  1:2881687/1‑49 (MQ=255)
                                                                 aGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGa  <  1:2621914/71‑1 (MQ=255)
                                                                 |                                                                      
CGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGA  >  Exported/2469920‑2470055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: