Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,526,945	C→T	8.4%	E13K (GAG→AAG)	ttk ←	division inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,526,945	0	C	T	8.4%	297.2 / 12.9	95	E13K (GAG→AAG)	ttk	division inhibitor
Reads supporting (aligned to +/- strand):  ref base C (48/38);  new base T (2/6);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACTT  >  Exported/2526878‑2527011                                                                    |                                                                   gCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTcgc                                                                  <  1:962632/70‑1 (MQ=255)    gtcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACg                                                              >  1:1285826/1‑71 (MQ=255)     tcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACgg                                                             >  1:532681/1‑71 (MQ=255)     tcgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTAGCGACg                                                              >  1:2584043/1‑70 (MQ=255)      cgtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTcg                                                                   >  1:1193401/1‑64 (MQ=255)       gtGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGt                                                            <  1:2758625/70‑1 (MQ=255)           tACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCtt                                                       >  1:1800783/1‑71 (MQ=255)           tACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCtt                                                       >  1:4744467/1‑71 (MQ=255)           tACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCtt                                                       >  1:7802/1‑71 (MQ=255)           tACGTTGGCTTCCATCGCTGGATTCCAGAATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCtt                                                       <  1:3194507/71‑1 (MQ=255)             cGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACg                                                              <  1:4352532/62‑1 (MQ=255)             cGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCttt                                                      <  1:3431908/70‑1 (MQ=255)                 ggCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCg                                                   >  1:3018574/1‑69 (MQ=255)                  gCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAg                                                <  1:2661752/71‑1 (MQ=255)                      ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttg                                            >  1:1060711/1‑71 (MQ=255)                      ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgttt                                             >  1:1041672/1‑70 (MQ=255)                      ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTcc                                                         >  1:4074351/1‑58 (MQ=255)                      ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttg                                            >  1:4068764/1‑71 (MQ=255)                      ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttg                                            >  1:4608987/1‑71 (MQ=255)                       cATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgt                                           <  1:1734409/71‑1 (MQ=255)                        aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:2233394/1‑49 (MQ=255)                        aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:940453/1‑49 (MQ=255)                        aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:2639744/1‑49 (MQ=255)                        aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCa                                                 >  1:2275305/1‑64 (MQ=255)                        aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAg                                                >  1:2085002/1‑65 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:1925519/1‑48 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:4427027/1‑48 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:4715429/1‑48 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:2734439/1‑48 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGa                                                                >  1:1609454/1‑48 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:4367988/71‑1 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:7312/71‑1 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         >  1:3464937/1‑71 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:3768998/71‑1 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:2527534/71‑1 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:1047476/71‑1 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         >  1:1145110/1‑71 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:1966736/71‑1 (MQ=255)                         tCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:1486042/71‑1 (MQ=255)                          cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:2374243/1‑71 (MQ=255)                          cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:4237596/1‑71 (MQ=255)                          cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:2183139/1‑71 (MQ=255)                          cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:4602865/1‑71 (MQ=255)                           gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:2955906/71‑1 (MQ=255)                           gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:2179143/71‑1 (MQ=255)                           gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       >  1:1297243/1‑71 (MQ=255)                           gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:3262430/71‑1 (MQ=255)                           gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        >  1:376724/1‑70 (MQ=255)                           gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        <  1:4392501/70‑1 (MQ=255)                           gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        <  1:2263185/70‑1 (MQ=255)                                   ccAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCAt                                <  1:4427608/70‑1 (MQ=255)                                   ccAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:3790523/1‑71 (MQ=255)                                   ccAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:3796166/1‑71 (MQ=255)                                    cAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:1324183/62‑1 (MQ=255)                                    cAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGt                              <  1:2685175/71‑1 (MQ=255)                                     tgCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGtttt                                        <  1:1145915/59‑1 (MQ=255)                                      gCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTa                            <  1:1974690/71‑1 (MQ=255)                                      gCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCt                                     >  1:3525384/1‑62 (MQ=255)                                      gCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTa                            >  1:1810528/1‑71 (MQ=255)                                        aTCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTAc                           >  1:1780927/1‑70 (MQ=255)                                        aTCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTAc                           >  1:3554421/1‑70 (MQ=255)                                        aTCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACa                          <  1:3154266/71‑1 (MQ=255)                                         tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:3489167/55‑1 (MQ=255)                                         tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:4087868/55‑1 (MQ=255)                                         tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:4027972/55‑1 (MQ=255)                                         tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:2175740/55‑1 (MQ=255)                                         tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:3458465/71‑1 (MQ=255)                                         tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:3456363/71‑1 (MQ=255)                                         tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:4615662/71‑1 (MQ=255)                                         tCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:1472561/71‑1 (MQ=255)                                           aGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaa                        <  1:3692048/70‑1 (MQ=255)                                            gcgcCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCa                                                 <  1:2421634/44‑1 (MQ=255)                                            gcgcCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCa                                                 <  1:1670476/44‑1 (MQ=255)                                            gcgcCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCa                                                 <  1:3124629/44‑1 (MQ=255)                                                cAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATAccc                  <  1:2893970/71‑1 (MQ=255)                                                 agagACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:4069922/49‑1 (MQ=255)                                                  gagaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCt                >  1:2156087/1‑71 (MQ=255)                                                   agaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       >  1:1512486/1‑47 (MQ=255)                                                   agaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       >  1:1051056/1‑47 (MQ=255)                                                   agaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGttttt                                       >  1:4218185/1‑47 (MQ=255)                                                   agaCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaa                         <  1:4012230/61‑1 (MQ=255)                                                     aCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGa              >  1:4601623/1‑70 (MQ=255)                                                     aCTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGa              >  1:2398505/1‑70 (MQ=255)                                                            tATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGc      <  1:1539343/71‑1 (MQ=255)                                                             aTTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaaa                       >  1:1837029/1‑53 (MQ=255)                                                              tttCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCa     <  1:1193602/70‑1 (MQ=255)                                                              tttCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCt                                     >  1:3736067/1‑38 (MQ=255)                                                              tttCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCt                                     >  1:563461/1‑38 (MQ=255)                                                              tttCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaaa                       <  1:2215292/52‑1 (MQ=255)                                                              tttCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCAc    <  1:2970288/71‑1 (MQ=255)                                                                tCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaaa                       >  1:690714/1‑50 (MQ=255)                                                                tCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACtt  >  1:3527913/1‑71 (MQ=255)                                                                 ccTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCt                                     >  1:2305299/1‑35 (MQ=255)                                                                 ccTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:1499026/1‑41 (MQ=255)                                                                 ccTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:3867605/1‑41 (MQ=255)                                                                 ccTCGCGACGGTTACTTTTCGCAGTTTGTTTTTCTGCCATg                               >  1:4077859/1‑41 (MQ=255)                                                                  cTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACa                          <  1:1338252/45‑1 (MQ=255)                                                                    |                                                                   GCCGTCGTGATACGTTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACTT  >  Exported/2526878‑2527011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑