| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,605,853 | T→C | 7.1% | V14V (GTT→GTC) | yhiO → | predicted universal stress (ethanol tolerance) protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,605,853 | 0 | T | C | 7.1% | 330.8 / 12.8 | 112 | V14V (GTT→GTC) | yhiO | predicted universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base T (49/55); new base C (2/6); total (51/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
CGCGGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATC > Exported/2605786‑2605923 | cgcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGttt < 1:3695629/69‑1 (MQ=255)cgcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGc < 1:2699819/71‑1 (MQ=255)cgcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTg < 1:1399040/70‑1 (MQ=255) gcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCa > 1:2226124/1‑71 (MQ=255) gcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCa > 1:1496111/1‑71 (MQ=255) gCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGt < 1:704888/71‑1 (MQ=255) aGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCa > 1:3168320/1‑65 (MQ=255) ggTCGCCGGGGAGGATGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTaa < 1:1150468/71‑1 (MQ=255) gTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAAc < 1:3941710/71‑1 (MQ=255) gCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATg < 1:2939025/71‑1 (MQ=255) gCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATg < 1:4475722/71‑1 (MQ=255) gCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATg < 1:3649621/71‑1 (MQ=255) ggggAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgc > 1:1823195/1‑70 (MQ=255) ggaggaGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgcg > 1:1495798/1‑71 (MQ=255) aggaggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACAt < 1:3100342/63‑1 (MQ=255) aggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAtt > 1:4231353/1‑71 (MQ=255) aggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGAGCTAtt > 1:3844417/1‑71 (MQ=255) aggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTAtt < 1:41619/71‑1 (MQ=255) ggTATGATAAGCACCGTCGCATTATTCTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTAttt < 1:4362680/71‑1 (MQ=255) gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt < 1:2823284/70‑1 (MQ=255) gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt < 1:3913248/70‑1 (MQ=255) gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt < 1:3141313/70‑1 (MQ=255) gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt < 1:3082478/70‑1 (MQ=255) gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTAttt < 1:1993955/70‑1 (MQ=255) aTGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTtct > 1:2602106/1‑70 (MQ=255) tGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtca < 1:2459954/71‑1 (MQ=255) gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTaa < 1:4574915/51‑1 (MQ=255) gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgcgc < 1:397494/61‑1 (MQ=255) gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgcgc < 1:4071931/61‑1 (MQ=255) gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtcat > 1:4341451/1‑71 (MQ=255) gATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtcat > 1:590313/1‑71 (MQ=255) gATAAGAACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgcgc < 1:1686343/61‑1 (MQ=255) aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGttt > 1:1152582/1‑40 (MQ=255) aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCa > 1:379477/1‑43 (MQ=255) aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACa > 1:712891/1‑52 (MQ=255) aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgc > 1:484582/1‑58 (MQ=255) aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcgc > 1:3094702/1‑58 (MQ=255) aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGgcg > 1:2762519/1‑57 (MQ=255) tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTa < 1:61033/48‑1 (MQ=255) tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTa < 1:617763/48‑1 (MQ=255) tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTa < 1:709791/48‑1 (MQ=255) tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACa > 1:2014679/1‑51 (MQ=255) aGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCAc > 1:3376771/1‑70 (MQ=255) aGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCAc < 1:3269238/70‑1 (MQ=255) aGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCAc < 1:2600549/70‑1 (MQ=255) aGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCAc < 1:2220569/70‑1 (MQ=255) cACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTa > 1:1140202/1‑70 (MQ=255) cGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcg > 1:3716225/1‑71 (MQ=255) gTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgc > 1:2695303/1‑71 (MQ=255) tCGCATTATTTTGGGCTTTATGTGTCGTTTGCAtt < 1:621678/35‑1 (MQ=255) gCATTATTTTGGGCTTTATGTGTCGTTTGCATTGt > 1:4411437/1‑35 (MQ=255) gCATTATTTTGGGCTTTATGTGTCGTTTGCATTGt > 1:3066167/1‑35 (MQ=255) gCATTATTTTGGGCTTTATGTGTCGTTTGCATTGt > 1:3471892/1‑35 (MQ=255) cATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttg < 1:1847372/71‑1 (MQ=255) attattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt < 1:537777/71‑1 (MQ=255) attattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt < 1:2200962/71‑1 (MQ=255) attattTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCtca < 1:4171367/55‑1 (MQ=255) attattTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt < 1:1845271/71‑1 (MQ=255) ttattTTTGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgtt < 1:1903260/71‑1 (MQ=255) ttattTTGGGCTTTATGTGTCGTTTGCATTTTTAACATGGCGCGCTATTTCTCATCACTa < 1:1614093/60‑1 (MQ=255) ttattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTa < 1:2268498/60‑1 (MQ=255) ttattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTa < 1:1939821/60‑1 (MQ=255) ttattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgtt < 1:858640/71‑1 (MQ=255) tattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAGGCGCgttgtt < 1:3364387/70‑1 (MQ=255) tattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgtt < 1:1382189/70‑1 (MQ=255) tattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt > 1:1418480/1‑69 (MQ=255) tattTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt > 1:4528177/1‑69 (MQ=255) attTTGGGCTTTATGTGTCGTTTGCATTGTTAACa > 1:1206025/1‑35 (MQ=255) attTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcg > 1:1639777/1‑60 (MQ=255) attTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTa > 1:4022085/1‑58 (MQ=255) ggCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTAc < 1:2883911/71‑1 (MQ=255) gCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACt < 1:2699538/71‑1 (MQ=255) cTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTGATCACTACGCGCGTTGTTAGTGGTACTg > 1:1401170/1‑71 (MQ=255) cTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTg > 1:1106465/1‑71 (MQ=255) cTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTg > 1:2628150/1‑71 (MQ=255) ttATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtcat < 1:2400953/43‑1 (MQ=255) ttATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCtcat < 1:459902/43‑1 (MQ=255) ttATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg > 1:1594219/1‑71 (MQ=255) tATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg > 1:456655/1‑70 (MQ=255) aTGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGt > 1:2886211/1‑70 (MQ=255) tgtgTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTa < 1:4400157/70‑1 (MQ=255) tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgc < 1:1859372/48‑1 (MQ=255) tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgc < 1:2593608/48‑1 (MQ=255) tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTAc > 1:420523/1‑62 (MQ=255) tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTAc > 1:863874/1‑62 (MQ=255) tgtCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTAc > 1:1080752/1‑62 (MQ=255) tCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTg < 1:3893072/70‑1 (MQ=255) cGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGc > 1:543716/1‑70 (MQ=255) gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgtt < 1:4412603/50‑1 (MQ=255) gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCg < 1:4606365/70‑1 (MQ=255) gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCg < 1:1607173/70‑1 (MQ=255) gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa > 1:2872250/1‑71 (MQ=255) gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa > 1:2898839/1‑71 (MQ=255) gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa > 1:182782/1‑71 (MQ=255) gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa > 1:1663187/1‑71 (MQ=255) gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa < 1:4034060/71‑1 (MQ=255) gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa > 1:4053040/1‑71 (MQ=255) tttGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGAt > 1:2893718/1‑71 (MQ=255) ttGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc > 1:2920243/1‑71 (MQ=255) ttGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc > 1:370315/1‑71 (MQ=255) | CGCGGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATC > Exported/2605786‑2605923 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |