| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,706,538 | T→C | 5.8% | S168P (TCT→CCT) | rpe → | D‑ribulose‑5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,706,538 | 0 | T | C | 5.8% | 512.4 / 17.0 | 174 | S168P (TCT→CCT) | rpe | D‑ribulose‑5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base T (72/91); new base C (2/8); total (74/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
Only 100 of 180 total aligned reads displayed.
TCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGAAATCGCTGCGGC > Exported/2706470‑2706608 | tCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCt > 1:3772017/1‑71 (MQ=255)tCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCt > 1:2105302/1‑71 (MQ=255)tCGGCGGGCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTc < 1:1243512/70‑1 (MQ=255) cggcggTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg > 1:1654908/1‑71 (MQ=255) cggcggTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg > 1:1598576/1‑71 (MQ=255) gTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCttt < 1:3917901/71‑1 (MQ=255) tCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTc < 1:2974329/63‑1 (MQ=255) tCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCtt < 1:4109114/69‑1 (MQ=255) cAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:4067033/1‑71 (MQ=255) cAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:247142/1‑71 (MQ=255) cAGTCTTTCATCCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:2206906/1‑71 (MQ=255) aGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa < 1:4145961/70‑1 (MQ=255) aGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa < 1:4607820/70‑1 (MQ=255) gTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACa < 1:2281490/71‑1 (MQ=255) tCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACa > 1:3308702/1‑70 (MQ=255) tCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACa > 1:1068758/1‑70 (MQ=255) cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACAtt > 1:1843422/1‑71 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGAc > 1:1895802/1‑67 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:3780140/1‑71 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:2763596/1‑71 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:4685116/1‑71 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:139026/1‑71 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg < 1:2803349/57‑1 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg < 1:2040573/57‑1 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:2200278/1‑71 (MQ=255) cATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa < 1:2659931/70‑1 (MQ=255) aTTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGGCGTATCGACGAGTCTGGCTTTGACATTc < 1:4469504/67‑1 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCt > 1:1114057/1‑55 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTg > 1:1508434/1‑58 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg > 1:4196852/1‑70 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg > 1:1964673/1‑70 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg < 1:3400935/70‑1 (MQ=255) cTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg < 1:2295857/69‑1 (MQ=255) cTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGaa < 1:760720/71‑1 (MQ=255) ctaaaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg < 1:2622016/66‑1 (MQ=255) aaaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGt > 1:1364876/1‑45 (MQ=255) aaaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGt > 1:2272130/1‑45 (MQ=255) aaaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCttt > 1:2591833/1‑53 (MQ=255) aaaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:126461/1‑55 (MQ=255) aaaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTg < 1:2988057/71‑1 (MQ=255) aaaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTg < 1:4022687/71‑1 (MQ=255) aaaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTg < 1:4414878/71‑1 (MQ=255) aaaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTg < 1:4091016/71‑1 (MQ=255) aaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTgg < 1:2490412/71‑1 (MQ=255) aaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTgg < 1:1845546/71‑1 (MQ=255) aaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTgg < 1:2672010/71‑1 (MQ=255) aaCACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTg < 1:2996010/70‑1 (MQ=255) acacTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGa > 1:3827845/1‑71 (MQ=255) acacTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGa > 1:576786/1‑71 (MQ=255) acacTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGa > 1:449519/1‑71 (MQ=255) acTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACg < 1:1685362/71‑1 (MQ=255) gATAAACTGCGGGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:2724500/1‑54 (MQ=255) gATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:425998/1‑54 (MQ=255) gATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTgg < 1:737658/71‑1 (MQ=255) gATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTgg < 1:3195006/71‑1 (MQ=255) gATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTg > 1:868261/1‑70 (MQ=255) aaaCTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACAt < 1:4133016/47‑1 (MQ=255) aaCTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCttt < 1:1325517/41‑1 (MQ=255) aaCTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCttt < 1:531097/41‑1 (MQ=255) aaCTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGt > 1:1011273/1‑70 (MQ=255) aaCTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGt > 1:2789421/1‑70 (MQ=255) aaCTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGt > 1:1761883/1‑70 (MQ=255) cTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:1647429/1‑41 (MQ=255) cTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACAt < 1:2785249/44‑1 (MQ=255) cTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGaa < 1:4616794/71‑1 (MQ=255) cgcgAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACa > 1:47435/1‑40 (MQ=255) cgcgAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAgg < 1:4371760/70‑1 (MQ=255) cgcgAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAgg < 1:482188/70‑1 (MQ=255) cgcgAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAgg < 1:2214630/70‑1 (MQ=255) cgcgAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAgg < 1:1380122/70‑1 (MQ=255) gcgAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTg < 1:4216817/71‑1 (MQ=255) gcgAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTg < 1:1048635/71‑1 (MQ=255) aaGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGTCGGTGGCGTGAAGGTGaac < 1:2744333/71‑1 (MQ=255) aaGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGaac < 1:4582469/71‑1 (MQ=255) aaGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGaac < 1:3054014/71‑1 (MQ=255) aaGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGaac < 1:4446603/71‑1 (MQ=255) tACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGaac < 1:1225475/68‑1 (MQ=255) aCGTCGACGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAacaaca > 1:2429/1‑71 (MQ=255) cGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACAt < 1:3926608/71‑1 (MQ=255) ccGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACg < 1:4473714/44‑1 (MQ=255) ccGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCg < 1:4736504/71‑1 (MQ=255) tATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGt < 1:1498856/43‑1 (MQ=255) tATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGaaa > 1:3901280/1‑71 (MQ=255) tATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGaaa > 1:2037463/1‑71 (MQ=255) tATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGaaa < 1:1948316/71‑1 (MQ=255) tATCGACGAGCCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGaaa < 1:922435/71‑1 (MQ=255) tATCGACGAGCCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGaaa < 1:4069425/71‑1 (MQ=255) tATCGACGAGCCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGaaa < 1:4547309/71‑1 (MQ=255) tATCGACGAGCCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGaaa < 1:2922335/71‑1 (MQ=255) gacgaGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAacaaca > 1:1101189/1‑58 (MQ=255) gacgaGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAacaaca > 1:288443/1‑58 (MQ=255) gacgaGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGAAATCGc > 1:1545798/1‑71 (MQ=255) acgaGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTg < 1:4444312/44‑1 (MQ=255) acgaGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTg < 1:645349/44‑1 (MQ=255) acgaGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTg < 1:3813330/44‑1 (MQ=255) acgaGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTg < 1:3492591/44‑1 (MQ=255) cgaGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGAAATCGCTg > 1:3641034/1‑71 (MQ=255) cgaGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGAAATCGCTg > 1:3091194/1‑71 (MQ=255) aGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAATGTGAACAACATTGGCGAAATCg < 1:3143104/66‑1 (MQ=255) tCTGGCTTTGACATTTGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGAAATCGCTgcggc > 1:4213167/1‑71 (MQ=255) | TCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGAAGTGGACGGTGGCGTGAAGGTGAACAACATTGGCGAAATCGCTGCGGC > Exported/2706470‑2706608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |