| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,714,239 | C→A | 8.4% | R41L (CGC→CTC) | nirB ← | nitrite reductase, large subunit, NAD(P)H‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,714,239 | 0 | C | A | 8.4% | 196.1 / 3.7 | 59 | R41L (CGC→CTC) | nirB | nitrite reductase, large subunit, NAD(P)H‑binding |
| Reads supporting (aligned to +/- strand): ref base C (20/34); new base A (3/2); total (23/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
CCAGCGACAGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCGA > Exported/2714171‑2714305 | ccAGCGACAGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgcgc < 1:1961186/71‑1 (MQ=255) cAGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt < 1:249699/71‑1 (MQ=255) aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt > 1:1103338/1‑70 (MQ=255) aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt > 1:4342326/1‑70 (MQ=255) aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt > 1:2187564/1‑70 (MQ=255) ctctTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTca > 1:3989097/1‑70 (MQ=255) ctTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTca > 1:2444233/1‑68 (MQ=255) ttCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTcaca < 1:512233/69‑1 (MQ=255) ttCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTcaca < 1:3094619/69‑1 (MQ=255) cggcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGaa < 1:222980/70‑1 (MQ=255) cggcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGaa < 1:3238156/70‑1 (MQ=255) cggcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGaa < 1:3390558/70‑1 (MQ=255) gtgtGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgc < 1:3064577/49‑1 (MQ=255) gtggtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgcgc > 1:2741894/1‑49 (MQ=255) gtggtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgcgc > 1:3418854/1‑49 (MQ=255) gtggtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgcgc > 1:3627277/1‑49 (MQ=255) gtggtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgcgc > 1:4011222/1‑49 (MQ=255) gtggtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgcgc > 1:669618/1‑49 (MQ=255) gtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGaaaa > 1:3122950/1‑62 (MQ=255) gtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGaaaa > 1:4564758/1‑62 (MQ=255) agagaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGaa < 1:1972748/57‑1 (MQ=255) agagaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCa > 1:1768867/1‑70 (MQ=255) agagaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCa > 1:962234/1‑70 (MQ=255) agaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGAAGAGCGGTTCTTCACAGAAAACGGTAATATCAAAg > 1:3350470/1‑71 (MQ=255) aaGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGtt < 1:865153/71‑1 (MQ=255) aaGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGtt < 1:4658361/71‑1 (MQ=255) aGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTg > 1:4338632/1‑71 (MQ=255) aGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTg > 1:3981127/1‑71 (MQ=255) tAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGc < 1:919989/71‑1 (MQ=255) tAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGc < 1:2189300/71‑1 (MQ=255) tAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGc < 1:1061651/71‑1 (MQ=255) tAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGc < 1:395385/71‑1 (MQ=255) tAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGc < 1:3437901/71‑1 (MQ=255) tAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGc > 1:570157/1‑71 (MQ=255) aaGACGAGAGGTGTACGCGGTCATAAGCGATGcgcg > 1:2648838/1‑36 (MQ=255) aGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCg > 1:4425477/1‑71 (MQ=255) cGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCAt < 1:4015/71‑1 (MQ=255) gagaGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGt < 1:2941942/50‑1 (MQ=255) agGTGTACGCGGTCATAAGCGATGCTCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCAt < 1:4720213/67‑1 (MQ=255) gtgtACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGt > 1:2142678/1‑56 (MQ=255) gtgtACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCa > 1:2106836/1‑67 (MQ=255) tACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCaa < 1:1775050/50‑1 (MQ=255) tACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTa < 1:46269/70‑1 (MQ=255) tACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTa < 1:998575/70‑1 (MQ=255) tACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTa < 1:1855812/70‑1 (MQ=255) cgGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCaag < 1:4470569/71‑1 (MQ=255) cgGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCaag < 1:177149/71‑1 (MQ=255) cgGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCaag < 1:1750855/71‑1 (MQ=255) tCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGa < 1:4561215/59‑1 (MQ=255) cATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCaaa > 1:334187/1‑43 (MQ=255) cATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCaag > 1:4424750/1‑67 (MQ=255) cATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCaag > 1:758019/1‑67 (MQ=255) aTAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCaag > 1:1412926/1‑66 (MQ=255) aTAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAagaaga < 1:626106/70‑1 (MQ=255) tAAGCGATTCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATc < 1:2173392/71‑1 (MQ=255) tAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATc < 1:1351960/53‑1 (MQ=255) tAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATc < 1:4555411/53‑1 (MQ=255) aGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCt < 1:2442472/70‑1 (MQ=255) aGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCt < 1:3243456/70‑1 (MQ=255) aGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCt < 1:3312184/70‑1 (MQ=255) aGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCt < 1:3332848/70‑1 (MQ=255) aGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCt < 1:4547196/70‑1 (MQ=255) aGCGATGCGCGGTTCTTCACAGAAAACGGTAATATAAAAGTTGGCCGCATCAGATTTATCAAGAAGATCt < 1:2441508/70‑1 (MQ=255) gCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTc > 1:4349892/1‑71 (MQ=255) cGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCg < 1:1774634/71‑1 (MQ=255) aTGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCGa > 1:3744804/1‑70 (MQ=255) aTGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCGa > 1:945788/1‑70 (MQ=255) | CCAGCGACAGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCGA > Exported/2714171‑2714305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |