| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,727,940 | T→C | 5.7% | F59F (TTT→TTC) | kefB → | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,727,940 | 0 | T | C | 5.7% | 262.2 / 5.6 | 87 | F59F (TTT→TTC) | kefB | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (32/50); new base C (2/3); total (34/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTTTGGC > Exported/2727875‑2728007 | gCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCgg < 1:2224816/70‑1 (MQ=255) aGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAAc > 1:4440741/1‑71 (MQ=255) gATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCg < 1:3511910/71‑1 (MQ=255) ttGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGc < 1:532429/71‑1 (MQ=255) ttGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGc < 1:2292576/71‑1 (MQ=255) cAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTg < 1:1715241/71‑1 (MQ=255) aaTTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGAAGAGATCCTCCACtttt > 1:4748749/1‑56 (MQ=255) aTTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTgg > 1:2939699/1‑70 (MQ=255) ggCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTAt > 1:4713408/1‑70 (MQ=255) ggCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTAt > 1:3526484/1‑70 (MQ=255) ggCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTAt > 1:2998394/1‑70 (MQ=255) cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCg < 1:3267309/57‑1 (MQ=255) cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCg < 1:2477674/57‑1 (MQ=255) cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCg < 1:420922/57‑1 (MQ=255) cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCt > 1:4493090/1‑70 (MQ=255) cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTg < 1:1099456/71‑1 (MQ=255) gTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCt < 1:4695740/69‑1 (MQ=255) ggggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATg < 1:2464811/70‑1 (MQ=255) ggggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATg < 1:493993/70‑1 (MQ=255) ggggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGt < 1:2425017/71‑1 (MQ=255) gggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTg < 1:2656203/66‑1 (MQ=255) ggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:4646396/71‑1 (MQ=255) ggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:4209521/71‑1 (MQ=255) ggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:317286/71‑1 (MQ=255) cTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:2060657/1‑64 (MQ=255) gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatcat < 1:3729955/71‑1 (MQ=255) gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatcat > 1:3483788/1‑71 (MQ=255) gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatca < 1:3147406/70‑1 (MQ=255) gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc < 1:330661/69‑1 (MQ=255) gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc < 1:1992675/69‑1 (MQ=255) ggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGc < 1:1412224/47‑1 (MQ=255) ggTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTc > 1:2162293/1‑44 (MQ=255) gTTTATTAGCGACGTCGATGAGATCCTCCACTTTTc > 1:3913278/1‑36 (MQ=255) tttATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCgg < 1:4257130/71‑1 (MQ=255) tattaGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCg < 1:3296839/44‑1 (MQ=255) tattaGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTgg < 1:964726/47‑1 (MQ=255) tattaGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGt < 1:1087633/48‑1 (MQ=255) attaGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGcc < 1:557554/70‑1 (MQ=255) ttaGCGACGTCGATGAGATCCTCCCCTTTTCGGAACTCGGCGTGGTATTc > 1:1413526/1‑50 (MQ=255) ttaGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt < 1:3621723/71‑1 (MQ=255) taGCGACGTCGATGAGATCCTCCACTTTTCGGAAc < 1:1298597/35‑1 (MQ=255) taGCGACGTCGATGAGATCCTCCACTTTTCGGAAc < 1:1750605/35‑1 (MQ=255) taGCGACGTCGATGAGATCCTCCACTTTTCGGAAc < 1:3482154/35‑1 (MQ=255) aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGGTGttt > 1:3363403/1‑57 (MQ=255) aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt > 1:4633393/1‑57 (MQ=255) aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt > 1:4226880/1‑57 (MQ=255) aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt > 1:2364593/1‑69 (MQ=255) aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt > 1:4210206/1‑69 (MQ=255) aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt > 1:4379704/1‑69 (MQ=255) aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTg < 1:387358/70‑1 (MQ=255) aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTg < 1:1930888/70‑1 (MQ=255) gACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatcatc > 1:2539653/1‑60 (MQ=255) gACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGtt < 1:4621741/71‑1 (MQ=255) aCGTCGATGAGATCCTCCACTTTTCGGAACTCGGCg < 1:3586715/36‑1 (MQ=255) aCGTCGATGAGATCCTCCACTTTTCGGAACTCGGCg < 1:1881973/36‑1 (MQ=255) aCGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTg < 1:1075409/71‑1 (MQ=255) cGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGa < 1:3429086/71‑1 (MQ=255) cGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGACTTGAGTTGa < 1:849008/71‑1 (MQ=255) gTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:532668/51‑1 (MQ=255) gTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:1190390/51‑1 (MQ=255) gTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGtt < 1:786480/50‑1 (MQ=255) tCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCt < 1:4213584/43‑1 (MQ=255) tCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTg < 1:2565740/63‑1 (MQ=255) tCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTg < 1:1374368/63‑1 (MQ=255) gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTa > 1:3127969/1‑36 (MQ=255) gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:4252973/1‑43 (MQ=255) gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:4154181/1‑43 (MQ=255) gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:704844/1‑43 (MQ=255) gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:2999009/1‑43 (MQ=255) gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGaa > 1:4703463/1‑68 (MQ=255) gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGaa > 1:4618819/1‑68 (MQ=255) gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATcc > 1:3647375/1‑71 (MQ=255) tGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCt < 1:300833/39‑1 (MQ=255) aTCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGc < 1:3817706/51‑1 (MQ=255) aTCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGc < 1:4343118/51‑1 (MQ=255) tcctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCaaa < 1:1922085/71‑1 (MQ=255) cctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc > 1:4174389/1‑43 (MQ=255) cctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCt > 1:4294094/1‑51 (MQ=255) cctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCt > 1:2574112/1‑51 (MQ=255) cctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAAc > 1:2568751/1‑71 (MQ=255) cctccACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGaa > 1:2982663/1‑60 (MQ=255) ctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAAc < 1:2078591/70‑1 (MQ=255) tccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACtt > 1:3124170/1‑71 (MQ=255) cACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc > 1:501783/1‑39 (MQ=255) cACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc > 1:643000/1‑39 (MQ=255) cTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc < 1:483864/37‑1 (MQ=255) cTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt < 1:2916384/46‑1 (MQ=255) cTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt < 1:3809123/46‑1 (MQ=255) ttttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTTTGGc > 1:4533831/1‑70 (MQ=255) tttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGa > 1:3556734/1‑46 (MQ=255) tttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGa > 1:3356565/1‑46 (MQ=255) tttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGa > 1:3409014/1‑46 (MQ=255) | GCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTTTGGC > Exported/2727875‑2728007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |