| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 232,790 | G→T | 5.6% | intergenic (‑29/+141) | hemB ← / ← ykiB | porphobilinogen synthase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 232,790 | 0 | G | T | 5.6% | 349.0 / 2.8 | 102 | intergenic (‑29/+141) | hemB/ykiB | porphobilinogen synthase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (55/41); new base T (4/2); total (59/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
AGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGA > Exported/232720‑232856 | agaTTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAggg < 1:4372453/71‑1 (MQ=255) gaTTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAgggg > 1:252135/1‑71 (MQ=255) tttGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCa < 1:3299892/71‑1 (MQ=255) ttGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAgggg > 1:721864/1‑68 (MQ=255) ttGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCa > 1:1408909/1‑70 (MQ=255) ttGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCa > 1:1575050/1‑70 (MQ=255) tGCGCAGGCGACGAGGGCGTTGGATTAAGTCAGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCa > 1:3778778/1‑69 (MQ=255) gcAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCAt > 1:3362738/1‑67 (MQ=255) cAGGCGACGAGGGCGTTTGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGtat < 1:3653228/71‑1 (MQ=255) aGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGtata > 1:2583104/1‑71 (MQ=255) aGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCAt < 1:2820226/57‑1 (MQ=255) aGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCAt < 1:338814/57‑1 (MQ=255) ggCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAg > 1:96774/1‑68 (MQ=255) ggCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAg > 1:1443165/1‑68 (MQ=255) gATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGta > 1:3599018/1‑52 (MQ=255) gATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGta > 1:4635853/1‑52 (MQ=255) gATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGta > 1:2663139/1‑52 (MQ=255) gATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGta > 1:2550627/1‑52 (MQ=255) gATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGta > 1:2365672/1‑52 (MQ=255) gATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGt > 1:1202689/1‑51 (MQ=255) gATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAggg < 1:4490560/71‑1 (MQ=255) tAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATTAAGGTGCACAGTATAcc > 1:1522775/1‑53 (MQ=255) tAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATTAAGGTGCACAGTATAcc > 1:1002260/1‑53 (MQ=255) tAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATTAAGGTGCACAGTATAcc > 1:2110716/1‑53 (MQ=255) tAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATTAAGGTGCACAGTATAcc > 1:4113251/1‑53 (MQ=255) tAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATg > 1:3559308/1‑71 (MQ=255) tAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATg > 1:2864531/1‑71 (MQ=255) tAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATg > 1:2784385/1‑71 (MQ=255) tAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATg > 1:2242289/1‑71 (MQ=255) aaGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATg < 1:2757172/70‑1 (MQ=255) aaGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGt > 1:257502/1‑71 (MQ=255) aaGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGt > 1:542502/1‑71 (MQ=255) aaGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGt > 1:759860/1‑71 (MQ=255) aaGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGt > 1:4366952/1‑71 (MQ=255) aaGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGt > 1:48067/1‑71 (MQ=255) aaGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGt > 1:1113323/1‑71 (MQ=255) aaGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGAGGt > 1:3705879/1‑71 (MQ=255) aaGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAAGGATGt > 1:2254463/1‑71 (MQ=255) aGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGt < 1:1426285/70‑1 (MQ=255) gTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGTGCATAGTATACCTGAAGCAGGGTAGGGATGttt < 1:2206511/71‑1 (MQ=255) gTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGttt < 1:4515843/71‑1 (MQ=255) tCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGtttt < 1:693487/71‑1 (MQ=255) tCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGtttt < 1:456355/71‑1 (MQ=255) tCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGtttt < 1:1269927/71‑1 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGCAGCAGGGTAGGGATGTTTTAc > 1:701344/1‑69 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGttt < 1:3695294/66‑1 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTAc < 1:3549365/69‑1 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACg > 1:2531425/1‑70 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACg > 1:4467977/1‑70 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACg > 1:217451/1‑70 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACg > 1:4462430/1‑70 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACg > 1:883900/1‑70 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa < 1:4033688/71‑1 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa < 1:4233431/71‑1 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa < 1:4450116/71‑1 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa < 1:144013/71‑1 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa < 1:1405090/71‑1 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa < 1:4715860/71‑1 (MQ=255) tCATGGTCTGCCTGATGTTTGTGGAATCCAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa < 1:3847946/71‑1 (MQ=255) aTGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa > 1:404155/1‑69 (MQ=255) aTGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa > 1:2841829/1‑69 (MQ=255) aTGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGa > 1:1639764/1‑69 (MQ=255) tGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGAt < 1:2981103/59‑1 (MQ=255) tGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGAt < 1:583889/59‑1 (MQ=255) tGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAg < 1:4199204/71‑1 (MQ=255) ggTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTa > 1:1924876/1‑64 (MQ=255) gTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGt > 1:287725/1‑59 (MQ=255) gTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGt > 1:2762000/1‑59 (MQ=255) gTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGtt > 1:4518488/1‑71 (MQ=255) gTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGtt > 1:299825/1‑71 (MQ=255) gTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGt > 1:2693535/1‑70 (MQ=255) tCTGCCTTATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTg < 1:781476/71‑1 (MQ=255) tCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGtttt < 1:4154612/61‑1 (MQ=255) tCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTg < 1:3805633/71‑1 (MQ=255) tCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTg < 1:632972/71‑1 (MQ=255) cTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGaaa > 1:3487748/1‑66 (MQ=255) cTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGaaa > 1:1179273/1‑66 (MQ=255) ccTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAg > 1:2619142/1‑40 (MQ=255) ccTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTcgc < 1:1160607/71‑1 (MQ=255) ccTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTcgc < 1:3896008/71‑1 (MQ=255) ccTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTcgc < 1:3822706/71‑1 (MQ=255) cTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTcgcg < 1:1052513/71‑1 (MQ=255) gATGTTTGTGGAATCGAGGGGCATAGTATTCCTGAAGCAGGGTAGGGATGTTTTACGa < 1:1887723/58‑1 (MQ=255) gATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGAt > 1:3613690/1‑71 (MQ=255) aTGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATg > 1:519332/1‑71 (MQ=255) aTGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATg > 1:4080572/1‑71 (MQ=255) gTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGt < 1:834206/70‑1 (MQ=255) tttGTTGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGtt < 1:936093/59‑1 (MQ=255) tgtgGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGa > 1:3296178/1‑70 (MQ=255) tgtgGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGa > 1:2796822/1‑70 (MQ=255) tgtgGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGa > 1:2398010/1‑70 (MQ=255) tgtgGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGa > 1:2098810/1‑70 (MQ=255) gAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACaaga < 1:2155618/71‑1 (MQ=255) aTCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACaag > 1:715872/1‑68 (MQ=255) tCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTc < 1:1210722/53‑1 (MQ=255) tCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACaagaa > 1:403665/1‑69 (MQ=255) tCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAagaaga < 1:421203/71‑1 (MQ=255) tCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAagaaga < 1:856505/71‑1 (MQ=255) gAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAgaga < 1:1007609/71‑1 (MQ=255) ggggCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTc < 1:4368246/49‑1 (MQ=255) | AGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGA > Exported/232720‑232856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |