Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 415,950 T→C 5.1% S257G (AGC→GGC) ‡ ybeZ ← hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported415,9500TC 5.1% 302.4 / 5.7 98S257G (AGC→GGC) ‡ybeZhypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base T (52/41);  new base C (3/2);  total (55/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 108 total aligned reads displayed.

TAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAAT  >  Exported/415881‑416019
                                                                     |                                                                     
tAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctc                                                                      >  1:2372690/1‑71 (MQ=255)
tAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctc                                                                      >  1:3090465/1‑71 (MQ=255)
  aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct                                                                     >  1:2930438/1‑70 (MQ=255)
  aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct                                                                     >  1:2842279/1‑70 (MQ=255)
  aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct                                                                     <  1:3918815/70‑1 (MQ=255)
  aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct                                                                     <  1:1882918/70‑1 (MQ=255)
    cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCtctc                                                                    >  1:1323739/1‑69 (MQ=255)
    cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCAt                                                                  >  1:1093753/1‑71 (MQ=255)
    cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCAt                                                                  >  1:78793/1‑71 (MQ=255)
    cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCAt                                                                  >  1:731179/1‑71 (MQ=255)
    cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTACATCTGTTCGATGGTGGTGTTCTGGCTCTCAt                                                                  >  1:3778705/1‑71 (MQ=255)
     gCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATc                                                                 >  1:1367924/1‑71 (MQ=255)
      cTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTTTTCGATGGTGGTGTTCTGGCTCTCATCg                                                                <  1:3951505/71‑1 (MQ=255)
      cTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATc                                                                 >  1:3432625/1‑70 (MQ=255)
      cTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCGGATCg                                                                >  1:2705049/1‑71 (MQ=255)
       ttttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATc                                                                 >  1:3404131/1‑69 (MQ=255)
         ttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag                                                              >  1:1800460/1‑70 (MQ=255)
         ttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag                                                              >  1:4240540/1‑70 (MQ=255)
         ttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag                                                              >  1:280283/1‑70 (MQ=255)
          tGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgaga                                                             <  1:202153/70‑1 (MQ=255)
          tGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                            >  1:1530854/1‑71 (MQ=255)
             gTTAAAACCGATACGGGTCATGAACATCTTCATCTGTTCGATGTTGGTGTTCTGGCTCTCAt                                                                  >  1:3607507/1‑62 (MQ=255)
               tAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGAt                                                        >  1:4357761/1‑70 (MQ=255)
                aaaaCCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa                                                       >  1:2623437/1‑70 (MQ=255)
                    ccGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAAcgc                                                  >  1:2976042/1‑71 (MQ=255)
                     cGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAAcgcg                                                 <  1:2875490/71‑1 (MQ=255)
                      gATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGcgt                                                <  1:2846761/71‑1 (MQ=255)
                          cGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGcgtcgt                                             <  1:4644514/70‑1 (MQ=255)
                            ggTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa                                          <  1:179884/71‑1 (MQ=255)
                                agtaaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt                                      <  1:2819391/68‑1 (MQ=255)
                                aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTGAGCGt                                      <  1:534097/71‑1 (MQ=255)
                                aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt                                      <  1:3891284/71‑1 (MQ=255)
                                aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt                                      <  1:3031544/71‑1 (MQ=255)
                                aGGAACATCTTCATCTGCTCGACGGTCGTGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTc                                           >  1:3525706/1‑66 (MQ=255)
                                aGGAACATCTTCATCTGCTCGACGGTCGTGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAgg                                        >  1:1431861/1‑68 (MQ=255)
                                 ggAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt                                      <  1:370660/70‑1 (MQ=255)
                                  gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa                                     >  1:3005192/1‑70 (MQ=255)
                                  gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa                                     >  1:4744366/1‑70 (MQ=255)
                                  gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                    >  1:2057428/1‑71 (MQ=255)
                                  gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                    >  1:3465459/1‑71 (MQ=255)
                                  gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                    <  1:1523595/71‑1 (MQ=255)
                                   aaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                    >  1:4577611/1‑70 (MQ=255)
                                   aaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                   <  1:3546143/71‑1 (MQ=255)
                                    aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                            >  1:2204159/1‑45 (MQ=255)
                                    aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                            >  1:2735683/1‑45 (MQ=255)
                                    aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                            >  1:2556841/1‑45 (MQ=255)
                                    aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                            >  1:2467472/1‑45 (MQ=255)
                                    aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                   <  1:1101927/70‑1 (MQ=255)
                                     cATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa                                                       <  1:4115012/49‑1 (MQ=255)
                                     cATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa                                                       <  1:4088829/49‑1 (MQ=255)
                                      aTCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCTTCGAGAATGATa                                                       <  1:3324702/48‑1 (MQ=255)
                                         ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCTTCGAGAATGATAAACGCGTCGTTCAGCGTAc                                    >  1:1895402/1‑64 (MQ=255)
                                         ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa                                                      >  1:3380947/1‑46 (MQ=255)
                                         ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa                                                      >  1:2655258/1‑46 (MQ=255)
                                         ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa                                     >  1:2112583/1‑63 (MQ=255)
                                         ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                    >  1:2840705/1‑64 (MQ=255)
                                         ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                    >  1:3156393/1‑64 (MQ=255)
                                           cATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc                              <  1:531802/68‑1 (MQ=255)
                                            aTCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCa                           <  1:3825442/70‑1 (MQ=255)
                                             tCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         >  1:1646717/1‑71 (MQ=255)
                                             tCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         >  1:3198458/1‑71 (MQ=255)
                                               tGTTCGATGGTGGTGTTCTGGCTCTCATCGAGGATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         >  1:1186726/1‑69 (MQ=255)
                                                 ttCGATGGTTGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         <  1:165374/67‑1 (MQ=255)
                                                 ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                   <  1:3809886/57‑1 (MQ=255)
                                                 ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         <  1:4196811/67‑1 (MQ=255)
                                                 ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         <  1:2727805/67‑1 (MQ=255)
                                                 ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         <  1:2711926/67‑1 (MQ=255)
                                                 ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         <  1:4695784/67‑1 (MQ=255)
                                                 atCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAACCGCGTCGTTCAGCGTACGACCACGCata                         <  1:3172875/66‑1 (MQ=255)
                                                   cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaaa                                                     >  1:2341350/1‑37 (MQ=255)
                                                   cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaaa                                                     >  1:3961490/1‑37 (MQ=255)
                                                   cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGCCCACGCATATAGGc                    >  1:588577/1‑70 (MQ=255)
                                                   cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGc                    >  1:1185104/1‑70 (MQ=255)
                                                    gATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc                   <  1:2045746/70‑1 (MQ=255)
                                                    gATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc                   <  1:1113756/70‑1 (MQ=255)
                                                     aTGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc                   <  1:4052627/69‑1 (MQ=255)
                                                      tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc                <  1:4750944/71‑1 (MQ=255)
                                                      tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc                <  1:3428267/71‑1 (MQ=255)
                                                      tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc                <  1:2613602/71‑1 (MQ=255)
                                                      tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc                <  1:2255534/71‑1 (MQ=255)
                                                      tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc                <  1:4197899/71‑1 (MQ=255)
                                                      tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc                >  1:1865383/1‑71 (MQ=255)
                                                      cggtcgtGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAGGGTGCGCCCGCGCATATAGGCCAGc                <  1:654269/70‑1 (MQ=255)
                                                        gtggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCg               <  1:2001596/70‑1 (MQ=255)
                                                         tggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt             <  1:2768528/71‑1 (MQ=255)
                                                         tggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt             <  1:840300/71‑1 (MQ=255)
                                                         gtgtgtTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc                              <  1:1532803/52‑1 (MQ=255)
                                                          ggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt             >  1:3322729/1‑70 (MQ=255)
                                                          ggtgTTCTGGCTCTAATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt             >  1:557101/1‑70 (MQ=255)
                                                           gtgtTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGc           >  1:849650/1‑71 (MQ=255)
                                                           gtgtGCTGGCTCTCATCGAGAATGATAAACGCGGCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGc           <  1:3044325/71‑1 (MQ=255)
                                                             gtTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAg                      >  1:4430137/1‑58 (MQ=255)
                                                                cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa                                          >  1:3556052/1‑35 (MQ=255)
                                                                cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         >  1:4221569/1‑52 (MQ=255)
                                                                cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         >  1:206683/1‑52 (MQ=255)
                                                                cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                         >  1:842776/1‑52 (MQ=255)
                                                                cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACtt      >  1:760054/1‑71 (MQ=255)
                                                                    ctctCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCat                          >  1:2979153/1‑47 (MQ=255)
                                                                    ctctCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCaa   >  1:2452422/1‑70 (MQ=255)
                                                                     tctcATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAAt  >  1:2887041/1‑70 (MQ=255)
                                                                     |                                                                     
TAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAAT  >  Exported/415881‑416019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: