| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 21,090 | A→G | 14.6% | D341G (GAC→GGC) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 21,090 | 0 | A | G | 14.6% | 121.4 / 13.2 | 48 | D341G (GAC→GGC) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (15/26); new base G (2/5); total (17/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
TCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCG > Exported/21021‑21145 | tCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgac < 1:3492836/71‑1 (MQ=255)tCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgac < 1:1719252/71‑1 (MQ=255) cTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacga < 1:4120248/71‑1 (MQ=255) cTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacga < 1:4534671/71‑1 (MQ=255) ggCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACt < 1:2321174/70‑1 (MQ=255) gCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAt < 1:4701468/71‑1 (MQ=255) cGATCACGTTACCCTGGATGCCGGTAGCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtg < 1:1336369/71‑1 (MQ=255) cGATCACGGTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtg < 1:1571065/71‑1 (MQ=255) aTCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtgtg < 1:4527248/71‑1 (MQ=255) cACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGAt > 1:600790/1‑71 (MQ=255) tACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtg > 1:3590110/1‑62 (MQ=255) tACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtg > 1:4141810/1‑62 (MQ=255) ccTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGa < 1:1079386/71‑1 (MQ=255) tGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacga > 1:644152/1‑52 (MQ=255) tGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacga > 1:3348854/1‑52 (MQ=255) tGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGaaa > 1:3253573/1‑71 (MQ=255) aTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATAc < 1:2018067/71‑1 (MQ=255) tGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATAc < 1:3749990/70‑1 (MQ=255) cGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaaa < 1:2513942/71‑1 (MQ=255) cGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaaa < 1:3082428/71‑1 (MQ=255) ggTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAAc > 1:481543/1‑71 (MQ=255) tGCCGTTCACACCGCCCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCg < 1:4735041/71‑1 (MQ=255) ccGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGc > 1:212399/1‑70 (MQ=255) ccGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCt < 1:14860/71‑1 (MQ=255) gTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTaa > 1:3128641/1‑71 (MQ=255) gTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTaa > 1:2863041/1‑71 (MQ=255) gTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGcc < 1:1669099/58‑1 (MQ=255) acacCGCGCCTGGCCACGGCCCGGACGACTATGTGAt < 1:1536775/37‑1 (MQ=255) acacCGCGCCTGGCCACGGCCCGGACGACTATGTGAt < 1:2562760/37‑1 (MQ=255) acacCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAAcc < 1:2363249/69‑1 (MQ=255) cacCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCgg < 1:1694309/71‑1 (MQ=255) ccGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTaa > 1:123316/1‑64 (MQ=255) cgcgCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTg > 1:604077/1‑71 (MQ=255) cgcgCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTg > 1:1056838/1‑71 (MQ=255) cgcgCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTg > 1:4585780/1‑71 (MQ=255) cgcCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCt < 1:3462547/59‑1 (MQ=255) cgcCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCt < 1:3869450/59‑1 (MQ=255) cgcCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCt < 1:210677/59‑1 (MQ=255) cgcCTGGCAACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGc > 1:1672451/1‑71 (MQ=255) ccTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGccc > 1:2686724/1‑71 (MQ=255) cTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCg < 1:1239595/71‑1 (MQ=255) cTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCg < 1:2071205/54‑1 (MQ=255) cTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCg < 1:374248/54‑1 (MQ=255) cTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCg < 1:900429/71‑1 (MQ=255) tGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAAc < 1:1757505/51‑1 (MQ=255) tGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAAc < 1:782503/51‑1 (MQ=255) tGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTaa > 1:430475/1‑57 (MQ=255) tGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCATGGAAAc < 1:2498692/51‑1 (MQ=255) ccACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGc < 1:4453288/40‑1 (MQ=255) ccACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGc < 1:3620182/40‑1 (MQ=255) ccACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGc < 1:3281073/40‑1 (MQ=255) ccACAGCCCGGGCGACTATGTGATCGGTCAGAAATACGGc < 1:2114790/40‑1 (MQ=255) ggACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCg > 1:2950984/1‑41 (MQ=255) ggACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCg > 1:829262/1‑41 (MQ=255) | TCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCG > Exported/21021‑21145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |