| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 56,544 | C→T | 9.4% | F131F (TTC→TTT) | yabP → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 56,544 | 0 | C | T | 9.4% | 293.6 / 16.4 | 96 | F131F (TTC→TTT) | yabP | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (49/38); new base T (4/5); total (53/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
GGTCGCAAAGGGCAATATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTGA > Exported/56475‑56612 | ggTCGCAAAGGGCAATATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCa > 1:469702/1‑71 (MQ=255) gTCGCAAAGGGCAATATGCGCTGGGGCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTc < 1:1437029/69‑1 (MQ=255) tCGCAAAGGGCAATATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCAtt < 1:3358187/71‑1 (MQ=255) cGCAAAGGGCAATATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCAtt > 1:4690115/1‑70 (MQ=255) gCAAAGGGCAATATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTg > 1:489263/1‑70 (MQ=255) gCAAAGGGCAATATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTg > 1:1117718/1‑70 (MQ=255) gCAAAGGGCAATATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTg > 1:327937/1‑70 (MQ=255) aaGGGCAATATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaa > 1:1584147/1‑69 (MQ=255) aaTATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATaa < 1:2055609/71‑1 (MQ=255) aaTATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATaa < 1:4619199/71‑1 (MQ=255) aaTATGCGCTGGGTCGCCCAGACCTTCGGGGACATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATaa < 1:1104590/71‑1 (MQ=255) atGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaaaa > 1:1883185/1‑62 (MQ=255) atGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaaaa > 1:84130/1‑62 (MQ=255) cgcTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATaa > 1:811922/1‑65 (MQ=255) cgcTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATaa > 1:4729870/1‑65 (MQ=255) cgcTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATaa > 1:165233/1‑65 (MQ=255) cgcTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATaa > 1:2473292/1‑65 (MQ=255) ggTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACt < 1:1819855/70‑1 (MQ=255) tCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaaaa < 1:1188338/52‑1 (MQ=255) gCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTa > 1:2392308/1‑64 (MQ=255) gCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTa > 1:849167/1‑64 (MQ=255) gCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGAc > 1:3436759/1‑71 (MQ=255) cccAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTTATTg < 1:4595198/45‑1 (MQ=255) ccAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGa > 1:4629363/1‑68 (MQ=255) cAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTTATTGaa > 1:1007545/1‑45 (MQ=255) cAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTTATTGaa > 1:2045320/1‑45 (MQ=255) aGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaaaa < 1:3235719/46‑1 (MQ=255) aGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaaaa < 1:3019956/46‑1 (MQ=255) aGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaaaa < 1:1070266/46‑1 (MQ=255) aGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaaaa < 1:1556745/46‑1 (MQ=255) aGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaaaa < 1:63330/46‑1 (MQ=255) gACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGc < 1:2365898/71‑1 (MQ=255) aCCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaa > 1:4678177/1‑42 (MQ=255) aCCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGaa > 1:1693478/1‑42 (MQ=255) aCCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACg > 1:921621/1‑66 (MQ=255) aCCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTg > 1:4377087/1‑69 (MQ=255) aCCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTg > 1:3691519/1‑69 (MQ=255) cTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAg > 1:4205571/1‑49 (MQ=255) cTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAg > 1:3093203/1‑49 (MQ=255) ttCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGa < 1:4055469/49‑1 (MQ=255) tCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGc < 1:3795267/66‑1 (MQ=255) cGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGata > 1:3288627/1‑49 (MQ=255) ggggATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAg > 1:4180739/1‑66 (MQ=255) ggggATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAg > 1:4364802/1‑66 (MQ=255) ggggATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAg > 1:1941517/1‑66 (MQ=255) gggATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:2440493/71‑1 (MQ=255) gggATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:3369572/71‑1 (MQ=255) ggATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATaa < 1:1997190/42‑1 (MQ=255) gATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGa > 1:4585517/1‑37 (MQ=255) gATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGa > 1:4152736/1‑37 (MQ=255) gATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt > 1:2378022/1‑69 (MQ=255) gATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATaa < 1:4630713/71‑1 (MQ=255) gATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATaa < 1:536881/71‑1 (MQ=255) gATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATaa < 1:2079596/71‑1 (MQ=255) atatCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAg > 1:2824192/1‑71 (MQ=255) atatCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAg > 1:135332/1‑71 (MQ=255) atatCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAg > 1:2712679/1‑71 (MQ=255) atatCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTAAGACGTTGCAGCTGCATAAg > 1:1588148/1‑71 (MQ=255) atCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATaa < 1:1343899/38‑1 (MQ=255) gAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGata > 1:1020549/1‑39 (MQ=255) gAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGata > 1:1422954/1‑39 (MQ=255) gAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCa < 1:3575704/71‑1 (MQ=255) gTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCa < 1:2346096/69‑1 (MQ=255) gTTAAGTGTCACTTTTTTCATTAAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAg < 1:3510159/71‑1 (MQ=255) aaGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTTCAGCTGCATAAGGGCCAAGGt > 1:583514/1‑70 (MQ=255) aaGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGt > 1:3498270/1‑70 (MQ=255) aGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTaa < 1:3937993/71‑1 (MQ=255) aGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTaa < 1:1503766/71‑1 (MQ=255) gtgtCACTTTTTTCATTGAAAAGAATAAGATATGTACTCa < 1:3944770/40‑1 (MQ=255) gtgtCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAAc > 1:151968/1‑71 (MQ=255) tgtCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACt < 1:390789/71‑1 (MQ=255) tCACTTTTTTTATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:3801598/53‑1 (MQ=255) tCACTTTTTTTATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:3448338/53‑1 (MQ=255) tCACTTTTTTTATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:2359591/53‑1 (MQ=255) tCACTTTTTTTATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:4529153/53‑1 (MQ=255) tCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:3786368/53‑1 (MQ=255) tCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:2560880/53‑1 (MQ=255) tCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:1296248/53‑1 (MQ=255) tCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCAt < 1:4561161/53‑1 (MQ=255) tCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATaa < 1:2979393/55‑1 (MQ=255) tCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTg < 1:3651535/71‑1 (MQ=255) aCTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTggg > 1:3427266/1‑71 (MQ=255) aCTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTggg > 1:2054571/1‑71 (MQ=255) aCTATTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATaa > 1:932266/1‑53 (MQ=255) ttttttCATTGAAAAGAATAAGATATGTACTCAGACGTTGc < 1:3741477/41‑1 (MQ=255) ttttttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATa > 1:1759797/1‑50 (MQ=255) ttttttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGt > 1:745301/1‑61 (MQ=255) ttttttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGt > 1:980857/1‑61 (MQ=255) ttttttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCg > 1:2805399/1‑71 (MQ=255) ttttttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCg > 1:3294855/1‑71 (MQ=255) ttttTATTGAAAAGAATAAGATATGTACTCAGACGTTGc > 1:2336347/1‑39 (MQ=255) ttttTATTGAAAAGAATAAGATATGTACTCAGACGTTGc > 1:163685/1‑39 (MQ=255) tttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGtt > 1:2909303/1‑70 (MQ=255) ttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTg < 1:2654737/70‑1 (MQ=255) ttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTg < 1:2735705/70‑1 (MQ=255) ttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTg < 1:4321020/70‑1 (MQ=255) ttCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTGa > 1:4582412/1‑71 (MQ=255) | GGTCGCAAAGGGCAATATGCGCTGGGTCGCCCAGACCTTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTGA > Exported/56475‑56612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |