Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 872,042 C→T 5.2% H681Y (CAT→TAT)  topA → DNA topoisomerase I, omega subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported872,0420CT 5.2% 324.8 / 5.3 96H681Y (CAT→TAT) topADNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand):  ref base C (37/54);  new base T (2/3);  total (39/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

AAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGT  >  Exported/871975‑872110
                                                                   |                                                                    
aaaaCGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATg                                                                   >  1:4514087/1‑71 (MQ=255)
aaaaCGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATg                                                                   >  1:94520/1‑71 (MQ=255)
 aaaCGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGt                                                                  >  1:2801845/1‑71 (MQ=255)
  aaCGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTc                                                                 >  1:1019438/1‑71 (MQ=255)
  aaCGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTc                                                                 >  1:3390272/1‑71 (MQ=255)
  aaCGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTc                                                                 >  1:2904774/1‑71 (MQ=255)
  aaCGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATACGAAACGTAAGTTGCATGTc                                                                 >  1:2270579/1‑71 (MQ=255)
    cgtcgtTGCCCGTAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCtg                                                               <  1:574921/71‑1 (MQ=255)
    cgtcgtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCtg                                                               >  1:4346688/1‑71 (MQ=255)
    cgtcgtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCtg                                                               <  1:2042120/71‑1 (MQ=255)
       cgtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTgg                                                            >  1:3576362/1‑71 (MQ=255)
       cgtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTgg                                                            >  1:1268001/1‑71 (MQ=255)
        gtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTgg                                                            <  1:4386911/70‑1 (MQ=255)
        gtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTgg                                                            <  1:948688/70‑1 (MQ=255)
        gtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTgg                                                            <  1:1339423/70‑1 (MQ=255)
        gtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGt                                                           <  1:2082564/71‑1 (MQ=255)
         ttGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                    <  1:671261/61‑1 (MQ=255)
         ttGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCtg                                                               <  1:4209022/66‑1 (MQ=255)
          tGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGtaa                                                         >  1:4319708/1‑71 (MQ=255)
           gCCCGAAATGCGGCACGGCGATGGCCAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGtaa                                                         >  1:424891/1‑70 (MQ=255)
           gCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGtaa                                                         >  1:4000535/1‑70 (MQ=255)
            cccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGtaata                                                       <  1:2805693/71‑1 (MQ=255)
            cccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGtaata                                                       <  1:2745180/71‑1 (MQ=255)
            cccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGt                                                           <  1:447037/67‑1 (MQ=255)
            cccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGt                                                           <  1:2760845/67‑1 (MQ=255)
            cccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGt                                                           <  1:1481710/67‑1 (MQ=255)
             ccGAAATGCGGCACGGCGATGTACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGtaataa                                                      <  1:1825204/71‑1 (MQ=255)
             ccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCa                                                                     >  1:2956229/1‑56 (MQ=255)
             ccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                    <  1:3880160/57‑1 (MQ=255)
             ccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                    <  1:2159030/57‑1 (MQ=255)
             ccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                    <  1:2205293/57‑1 (MQ=255)
             ccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                    <  1:3070240/57‑1 (MQ=255)
             ccGAAATGCGGCACGCCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                    <  1:2247090/57‑1 (MQ=255)
              cGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                    <  1:26664/56‑1 (MQ=255)
              cGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                    <  1:524585/56‑1 (MQ=255)
               gAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAAcc                                                    <  1:38852/71‑1 (MQ=255)
                taaTGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTTGTAATAAccc                                                   <  1:2904405/70‑1 (MQ=255)
                 aaTGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:2281434/71‑1 (MQ=255)
                  aTGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAAccc                                                   <  1:4503912/69‑1 (MQ=255)
                  aTGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAAccc                                                   <  1:2261764/69‑1 (MQ=255)
                             cGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGt                                      <  1:963644/71‑1 (MQ=255)
                                tGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTAc                                   >  1:1940567/1‑71 (MQ=255)
                                 ggACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTAc                                   >  1:4651009/1‑70 (MQ=255)
                                 ggACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTAc                                   >  1:2971782/1‑70 (MQ=255)
                                  gACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                    >  1:3817387/1‑36 (MQ=255)
                                   aCAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACgag                                <  1:3382522/71‑1 (MQ=255)
                                    cAGCTATCTCATCGATGCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACgag                                <  1:307243/70‑1 (MQ=255)
                                       cTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:1994103/49‑1 (MQ=255)
                                       cTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:4628255/49‑1 (MQ=255)
                                       cTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:776652/49‑1 (MQ=255)
                                        tATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCg                            >  1:262166/1‑70 (MQ=255)
                                        tATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCg                            >  1:2490986/1‑70 (MQ=255)
                                          tctcATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGaa                          <  1:985831/70‑1 (MQ=255)
                                           ctcATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:1681524/45‑1 (MQ=255)
                                            tcATCGATCCGAAACGTAAGTTGTATGTCTGTGGTAATAACCCAACCTGCGACGGTTAc                                   >  1:1321222/1‑59 (MQ=255)
                                            tcATCGATCCGAAACGTAAGTTGTATGTCTGTGGTAATAACCCAACCTGCGACGGTTAc                                   >  1:1731388/1‑59 (MQ=255)
                                            tcATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTAc                                   >  1:995084/1‑59 (MQ=255)
                                              aTCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTAc                                   <  1:4650339/57‑1 (MQ=255)
                                              aTCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAggg                     >  1:159801/1‑71 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAAcc                                                    <  1:2160213/36‑1 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAAcc                                                    <  1:490468/36‑1 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:2058935/38‑1 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:1946466/38‑1 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:545090/38‑1 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:1808814/38‑1 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCa                                                  <  1:1101094/38‑1 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCg                            <  1:3842521/60‑1 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCg                            <  1:2883495/60‑1 (MQ=255)
                                                  aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCg                            <  1:741493/60‑1 (MQ=255)
                                                    ccGAAACGTAAGTTGCATGTCTGTGGTAATTACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAAtt               >  1:2492165/1‑71 (MQ=255)
                                                    ccGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAAtt               >  1:2820885/1‑71 (MQ=255)
                                                    ccGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAAtt               >  1:2230817/1‑71 (MQ=255)
                                                    ccGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAAt                >  1:2497789/1‑70 (MQ=255)
                                                    ccGAAAAGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAggg                     >  1:3277512/1‑65 (MQ=255)
                                                       aagcGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTcc             >  1:1994420/4‑70 (MQ=255)
                                                       aaaCGTAAGTTGTATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCg            <  1:4515913/71‑1 (MQ=255)
                                                       aaaCGTAAGTTGTATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCg            <  1:3992741/71‑1 (MQ=255)
                                                       aaaCGTAAGTTGTATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCg            <  1:3963991/71‑1 (MQ=255)
                                                       aaaCGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCg            <  1:1158749/71‑1 (MQ=255)
                                                            tAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTa       <  1:2114905/71‑1 (MQ=255)
                                                             aaGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCAtt        >  1:2110577/1‑69 (MQ=255)
                                                             aaGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTa       >  1:3068325/1‑70 (MQ=255)
                                                               gTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGAt                              >  1:464405/1‑45 (MQ=255)
                                                               gTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGAt                              >  1:1326901/1‑45 (MQ=255)
                                                                ttGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCg                            <  1:3071364/46‑1 (MQ=255)
                                                                ttGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCg                            <  1:3921790/46‑1 (MQ=255)
                                                                ttGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCg                            <  1:1967849/46‑1 (MQ=255)
                                                                  gCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGaa                          >  1:267922/1‑46 (MQ=255)
                                                                  gCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGaa                          >  1:1510908/1‑46 (MQ=255)
                                                                  gCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAaga                        >  1:1381243/1‑48 (MQ=255)
                                                                  gCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAaga                        >  1:2595282/1‑48 (MQ=255)
                                                                  gCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGt  <  1:179721/70‑1 (MQ=255)
                                                                  gCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGt  <  1:1922468/70‑1 (MQ=255)
                                                                  gCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGt  >  1:760719/1‑70 (MQ=255)
                                                                  gCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGt  <  1:3553866/70‑1 (MQ=255)
                                                                  gCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGt  <  1:4678412/70‑1 (MQ=255)
                                                                   |                                                                    
AAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGT  >  Exported/871975‑872110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: