| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,008,960 | G→A | 5.0% | L98L (CTG→CTA) | folM → | dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,008,960 | 0 | G | A | 5.0% | 337.3 / 4.3 | 99 | L98L (CTG→CTA) | folM | dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base G (46/48); new base A (3/2); total (49/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
CACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGA > Exported/1008891‑1009028 | cACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTgg < 1:4579589/71‑1 (MQ=255) cATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGc < 1:2672233/68‑1 (MQ=255) cATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGc < 1:1632310/68‑1 (MQ=255) cATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGa > 1:2804474/1‑71 (MQ=255) aTGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGAc > 1:3224818/1‑71 (MQ=255) tGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCg > 1:928193/1‑68 (MQ=255) tGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCg > 1:3933186/1‑68 (MQ=255) tGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCg > 1:1858150/1‑68 (MQ=255) tGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg < 1:1273918/71‑1 (MQ=255) tGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg < 1:2918035/71‑1 (MQ=255) tGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg < 1:1238998/71‑1 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTa > 1:3663118/1‑71 (MQ=255) gTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCt < 1:3939949/71‑1 (MQ=255) gTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCt < 1:2966178/71‑1 (MQ=255) tATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCa > 1:1387903/1‑71 (MQ=255) ttttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat > 1:1658710/1‑70 (MQ=255) ttttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat > 1:4145068/1‑70 (MQ=255) ttttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat > 1:1990568/1‑70 (MQ=255) tttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:1097852/1‑71 (MQ=255) tttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:4270123/1‑71 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgat > 1:897363/1‑71 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgat > 1:4367805/1‑71 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga < 1:2587649/70‑1 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga < 1:1553065/70‑1 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga < 1:2403078/70‑1 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga < 1:4413016/70‑1 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACGGGCCGACGTACTGGCTTGCatga < 1:314733/70‑1 (MQ=255) gCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:2793170/1‑70 (MQ=255) gCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:2888041/1‑70 (MQ=255) cATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGc < 1:1554225/70‑1 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAGCCGCGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:2714707/1‑66 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:4720066/1‑66 (MQ=255) aCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATc < 1:3659324/71‑1 (MQ=255) cGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATc > 1:252531/1‑70 (MQ=255) cGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCAACTGGCCGACGTACTGGCTTGCATGATGCAGATc < 1:185469/70‑1 (MQ=255) gCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCa > 1:1569163/1‑71 (MQ=255) ccAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGCTTGCATGATGCAGATCCAc < 1:2123801/71‑1 (MQ=255) ccAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGCTTGCATGATGCAGATCCAc < 1:2939786/71‑1 (MQ=255) cAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACg > 1:4079690/1‑71 (MQ=255) gCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa > 1:1462320/1‑71 (MQ=255) cGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:1153734/70‑1 (MQ=255) cGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:1016509/70‑1 (MQ=255) cGTGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGCtt > 1:3448388/1‑48 (MQ=255) cGTGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGCTTGCATGATGCAGa > 1:1792675/1‑60 (MQ=255) cGTGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGCTTGCATGATGCAGa > 1:4493116/1‑60 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCtt < 1:2601280/46‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg < 1:1574470/54‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg < 1:4493742/54‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:2803939/50‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:2390415/50‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:3196931/50‑1 (MQ=255) tGGATGGCAGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCtt < 1:2090908/46‑1 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGc > 1:4454276/1‑46 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGc > 1:848405/1‑46 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa > 1:1689357/1‑66 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa > 1:2604367/1‑66 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa > 1:3886913/1‑66 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAccc < 1:2124021/71‑1 (MQ=255) tGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGt < 1:3260441/61‑1 (MQ=255) tGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAcccc > 1:2900807/1‑70 (MQ=255) gCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa > 1:854364/1‑62 (MQ=255) gCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa > 1:2568274/1‑62 (MQ=255) aaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAcc < 1:3786031/59‑1 (MQ=255) aaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCt > 1:1426609/1‑70 (MQ=255) aaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTc < 1:1408693/71‑1 (MQ=255) ccGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAcccc > 1:3466103/1‑59 (MQ=255) cGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATa > 1:691373/1‑54 (MQ=255) cGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATa > 1:2033369/1‑54 (MQ=255) cGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATa > 1:2020538/1‑54 (MQ=255) cGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATa > 1:2863661/1‑54 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCt < 1:1435067/62‑1 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCa > 1:1051235/1‑71 (MQ=255) gTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGc > 1:4665508/1‑63 (MQ=255) gTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGc > 1:1710272/1‑63 (MQ=255) gTGCGACACTGGCCGACGTACTGGCTTGCATGATGCAGATc > 1:768596/1‑41 (MQ=255) gcgcCACTGGCCGACGTACTGGCTTGCATGATGCAGATc < 1:4402725/39‑1 (MQ=255) cgcCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATgc < 1:4434162/70‑1 (MQ=255) cgcCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATgc < 1:4088770/70‑1 (MQ=255) cgcCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATgc < 1:3606893/70‑1 (MQ=255) cgcCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATgc < 1:3861645/70‑1 (MQ=255) cACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTg < 1:3348692/71‑1 (MQ=255) cACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTg < 1:746440/71‑1 (MQ=255) cACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTg > 1:2093263/1‑71 (MQ=255) cTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGt < 1:894726/38‑1 (MQ=255) cTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGt < 1:3276777/38‑1 (MQ=255) cTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAAc < 1:3262008/60‑1 (MQ=255) cTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGa < 1:4585411/71‑1 (MQ=255) cTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGa < 1:1909740/71‑1 (MQ=255) cTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGa < 1:774284/71‑1 (MQ=255) tGGCCGACGTACTGGCTTGCATGATGCAGATCCACGt < 1:652489/37‑1 (MQ=255) tGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCa < 1:2576300/57‑1 (MQ=255) tGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCa < 1:514726/57‑1 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTa > 1:1602410/1‑38 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTa > 1:3015136/1‑38 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTa > 1:1773319/1‑38 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTa > 1:589265/1‑38 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCa > 1:2416218/1‑46 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCa > 1:109412/1‑46 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGa > 1:3967226/1‑69 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGa > 1:2043318/1‑69 (MQ=255) | CACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGA > Exported/1008891‑1009028 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |