| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,056,210 | T→C | 8.5% | L58S (TTA→TCA) | rnt → | ribonuclease T (RNase T) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,056,210 | 0 | T | C | 8.5% | 171.0 / 6.9 | 59 | L58S (TTA→TCA) | rnt | ribonuclease T (RNase T) |
| Reads supporting (aligned to +/- strand): ref base T (32/22); new base C (3/2); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
CTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGAC > Exported/1056143‑1056280 | ctgctTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacattaca > 1:616622/1‑71 (MQ=255) gccATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAAc > 1:2788940/1‑70 (MQ=255) ccATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCa < 1:2189330/71‑1 (MQ=255) tCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAttt < 1:1393879/71‑1 (MQ=255) aCCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacatta > 1:2977355/1‑48 (MQ=255) aCCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacatta > 1:2024145/1‑48 (MQ=255) aCCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTg > 1:1211258/1‑70 (MQ=255) cTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCgg < 1:2414491/71‑1 (MQ=255) tGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacatta < 1:569219/44‑1 (MQ=255) tGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacatta < 1:418137/44‑1 (MQ=255) gAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAttt > 1:3350610/1‑64 (MQ=255) gAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAttt > 1:2916340/1‑64 (MQ=255) gAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAttt > 1:2155198/1‑64 (MQ=255) gAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAttt > 1:215400/1‑64 (MQ=255) gAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAttt > 1:2145941/1‑64 (MQ=255) gAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAttt > 1:3670762/1‑64 (MQ=255) gAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGgcg > 1:3387679/1‑71 (MQ=255) gAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGgcg > 1:2368650/1‑71 (MQ=255) gAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGgcg > 1:1343481/1‑71 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCacattaca > 1:2001245/1‑44 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCacattaca > 1:3999127/1‑44 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCa > 1:2969800/1‑60 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:4448545/1‑61 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:798419/1‑61 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:3967811/1‑61 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:1052329/1‑61 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:1400188/1‑61 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:3550256/1‑61 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGgcg > 1:3761988/1‑70 (MQ=255) aaaaTGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCAAGTCGAACCa > 1:3769909/1‑60 (MQ=255) ggATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAAtt > 1:1315773/1‑71 (MQ=255) tGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGc < 1:491971/71‑1 (MQ=255) gAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCa > 1:3434377/1‑51 (MQ=255) gAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:2243584/1‑52 (MQ=255) gAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:3185869/1‑52 (MQ=255) gAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:3682657/1‑52 (MQ=255) gAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:3806154/1‑52 (MQ=255) gAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAt > 1:1115626/1‑52 (MQ=255) aaCAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCaa > 1:4647173/1‑71 (MQ=255) aaGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCa < 1:1052445/71‑1 (MQ=255) aaGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCa < 1:2896613/71‑1 (MQ=255) aaGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCa < 1:2653759/71‑1 (MQ=255) aGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAg < 1:4057981/71‑1 (MQ=255) aGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAg < 1:1469490/71‑1 (MQ=255) tggctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGc > 1:2355935/1‑71 (MQ=255) gctgATGCCGGACACCACATCACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAAc < 1:4033886/62‑1 (MQ=255) gctgATGCCGGACACCACATCACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAAc < 1:3601316/62‑1 (MQ=255) ctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCt < 1:188715/71‑1 (MQ=255) tgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCt < 1:4171968/70‑1 (MQ=255) tgATGCCGGACACCACATCACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCa > 1:2649682/1‑62 (MQ=255) tgATGCCGGACACCACATCACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCa > 1:413690/1‑62 (MQ=255) tgATGCCGGACACCACATCACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCa > 1:1410764/1‑62 (MQ=255) aTGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCaaa < 1:3040750/50‑1 (MQ=255) aTGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCaaa < 1:2552696/50‑1 (MQ=255) aTGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCaaa < 1:2388063/50‑1 (MQ=255) aTGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCaaa < 1:1569300/50‑1 (MQ=255) aTGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCaaa < 1:143697/50‑1 (MQ=255) aTGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCaaa < 1:1524777/50‑1 (MQ=255) tGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGcc > 1:4665482/1‑71 (MQ=255) ggACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGaa > 1:3569238/1‑58 (MQ=255) ggACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGa > 1:1080996/1‑57 (MQ=255) accacATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGc < 1:823894/62‑1 (MQ=255) accacATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGc < 1:656029/62‑1 (MQ=255) accacATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGc < 1:2194411/62‑1 (MQ=255) accacATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGc < 1:4730323/62‑1 (MQ=255) ccacATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGc < 1:3584941/71‑1 (MQ=255) cattacattTCCACGTCGAACCATTTGTCGGCGCAAAt < 1:3551749/38‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAAttt > 1:956512/1‑38 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAAttt > 1:73332/1‑38 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAAttt > 1:3694897/1‑38 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGc < 1:2083507/40‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGc < 1:182162/40‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCa < 1:609277/45‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCa < 1:4157094/45‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAg < 1:1200335/46‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAg < 1:1188985/46‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAg < 1:1367049/49‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAg < 1:1482256/49‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAg < 1:683901/49‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAg < 1:2894261/49‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAg < 1:3475015/49‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAg < 1:4210341/49‑1 (MQ=255) ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGa < 1:3795036/71‑1 (MQ=255) ttacattTCAACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAg < 1:1277537/46‑1 (MQ=255) ttacataTCCACGTCGAACCATTTGTAGGCGCAAAtt > 1:3928467/1‑37 (MQ=38) tacattTCCACGTCGAACCATTTTTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAAAGGCATTGAc > 1:4327884/1‑71 (MQ=255) tacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGAc > 1:945281/1‑71 (MQ=255) | CTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGAC > Exported/1056143‑1056280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |