| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,368,720 | T→C | 10.4% | N232S (AAC→AGC) | dusC ← | tRNA‑dihydrouridine synthase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,368,720 | 0 | T | C | 10.4% | 126.7 / 6.2 | 48 | N232S (AAC→AGC) | dusC | tRNA‑dihydrouridine synthase C |
| Reads supporting (aligned to +/- strand): ref base T (22/21); new base C (2/3); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-01 | |||||||||||
CAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCG > Exported/1368659‑1368789 | caaagcaaCCACCTCCGTCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAatat < 1:4645443/71‑1 (MQ=255) aaagcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATAtt < 1:3566500/71‑1 (MQ=255) aaagcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATAtt < 1:3560962/71‑1 (MQ=255) aaagcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGCTGGGAATAtt < 1:3550892/71‑1 (MQ=255) gcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAg > 1:615858/1‑71 (MQ=255) accaccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAgcgc > 1:1686451/1‑71 (MQ=255) accaccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAgcgc > 1:609355/1‑71 (MQ=255) accaccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAgcgc > 1:3085608/1‑71 (MQ=255) accaccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAgcgc > 1:4170527/1‑71 (MQ=255) ccGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGa > 1:3466078/1‑71 (MQ=255) cGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGAc < 1:3153817/71‑1 (MQ=255) cGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGAc < 1:2893738/71‑1 (MQ=255) aCGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGcc < 1:1224757/59‑1 (MQ=255) ggCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAatcat > 1:4613431/1‑71 (MQ=255) cATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGaa < 1:3192622/44‑1 (MQ=255) ttCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGaa < 1:369348/42‑1 (MQ=255) cgcgGTTCGTTATATTTTACCACCCGGCTCAGGTTggg > 1:20790/1‑38 (MQ=255) cgcgGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCa > 1:4567984/1‑60 (MQ=255) cgcgGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCa > 1:1912201/1‑60 (MQ=255) cgcgGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCACGCGACCAATCATCACTg > 1:2841584/1‑70 (MQ=255) tCGTTATATTTTACCACCCGGCTCAGGCTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCg > 1:3916813/1‑69 (MQ=255) tCGTTATATTTTACCACCCGGCTCAGGCTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCg > 1:652390/1‑69 (MQ=255) gTTATATTTTACCACCCGGCTCAGGCTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGc < 1:3255524/71‑1 (MQ=255) ttATATTTTACCACCCGGCTCAGGCTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGc < 1:1239040/70‑1 (MQ=255) atatTTTACCACCCGGCTCAGTTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGc > 1:955076/1‑71 (MQ=255) atatTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGc > 1:3707906/1‑71 (MQ=255) atatTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGc > 1:4670018/1‑71 (MQ=255) atatTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGc > 1:4704090/1‑71 (MQ=255) atatTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGc > 1:1111625/1‑71 (MQ=255) ttttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTg < 1:3589003/70‑1 (MQ=255) ttttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGa > 1:3273622/1‑71 (MQ=255) accaccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCg < 1:839636/70‑1 (MQ=255) ccaccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGcc < 1:2013356/71‑1 (MQ=255) ccGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATg < 1:2984417/70‑1 (MQ=255) ggCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCAt < 1:1129491/71‑1 (MQ=255) ggCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCAt < 1:1708137/71‑1 (MQ=255) ggCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCAt < 1:173425/71‑1 (MQ=255) ggCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCAt < 1:2459821/71‑1 (MQ=255) gCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCAt < 1:2609386/70‑1 (MQ=255) cTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCAttg < 1:2572924/71‑1 (MQ=255) cTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCAttg < 1:635157/71‑1 (MQ=255) aGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGc < 1:2789551/41‑1 (MQ=255) aGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGc < 1:3047227/41‑1 (MQ=255) gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGt > 1:2786194/1‑41 (MQ=255) gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGt > 1:4273668/1‑41 (MQ=255) gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGt > 1:4111821/1‑41 (MQ=255) gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGt > 1:945770/1‑41 (MQ=255) gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTgcg > 1:1294738/1‑71 (MQ=255) | CAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCG > Exported/1368659‑1368789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |