| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 139,335 | G→A | 9.0% | P278S (CCG→TCG) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 139,335 | 0 | G | A | 9.0% | 309.6 / 15.3 | 100 | P278S (CCG→TCG) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base G (57/34); new base A (7/2); total (64/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTT > Exported/139266‑139397 | gCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCgg < 1:2075532/70‑1 (MQ=255) gTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAgg > 1:3902459/1‑71 (MQ=255) aTAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa > 1:1599137/1‑71 (MQ=255) aTAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa > 1:1408836/1‑71 (MQ=255) tAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa > 1:3885111/1‑70 (MQ=255) gTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACAt > 1:2228083/1‑71 (MQ=255) tGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATg < 1:4600873/71‑1 (MQ=255) tGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATg < 1:975983/71‑1 (MQ=255) tGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATg < 1:2442871/71‑1 (MQ=255) aCTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCg < 1:4042108/71‑1 (MQ=255) tGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa > 1:4144663/1‑61 (MQ=255) tGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa > 1:3039355/1‑61 (MQ=255) tGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTg > 1:3825996/1‑71 (MQ=255) tGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTg > 1:4487360/1‑71 (MQ=255) tGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGAAGGCAACATGCGGCTg > 1:2568711/1‑71 (MQ=255) gCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGt < 1:2127702/70‑1 (MQ=255) gTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCa < 1:717821/66‑1 (MQ=255) tATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGtat < 1:4231423/71‑1 (MQ=255) tATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGtat < 1:3386226/71‑1 (MQ=255) tATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGta > 1:3757338/1‑70 (MQ=255) ttGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGc < 1:1742441/48‑1 (MQ=255) ttGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGtata > 1:3287796/1‑70 (MQ=255) tGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa < 1:2093089/49‑1 (MQ=255) tGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa < 1:1432889/49‑1 (MQ=255) tGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa < 1:3309256/49‑1 (MQ=255) tGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa < 1:4061139/49‑1 (MQ=255) ggCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAAc > 1:3876425/1‑71 (MQ=255) ggCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAAc > 1:2697481/1‑71 (MQ=255) ggCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAAc > 1:4040824/1‑71 (MQ=255) ggCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGAAGGCAACATGCGGCTGTCACTGta > 1:1298064/1‑66 (MQ=255) ggCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGAAGGCAACATGCGGCTGTCACTGTATAAAc > 1:1626902/1‑71 (MQ=255) ggCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGAAGGCAACATGCGGCTGTCACTGTATAAAc > 1:904312/1‑71 (MQ=255) gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACa > 1:572358/1‑49 (MQ=255) gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACa > 1:2116453/1‑49 (MQ=255) gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACa > 1:1788414/1‑49 (MQ=255) gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACa > 1:744579/1‑49 (MQ=255) gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAAc < 1:740467/70‑1 (MQ=255) gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACg < 1:327914/71‑1 (MQ=255) gCAACGCCATGAATGATAGGCGCAAAGCTCGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAAc < 1:924773/70‑1 (MQ=255) aCGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGCGGCAACATGCGGCTGTc > 1:4383988/1‑56 (MQ=255) aCGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAAt > 1:4492897/1‑71 (MQ=255) aCGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAAt > 1:142534/1‑71 (MQ=255) cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCgg > 1:3045526/1‑37 (MQ=255) cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATg > 1:1022021/1‑71 (MQ=255) gCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGc < 1:2319953/71‑1 (MQ=255) gCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGc < 1:128188/71‑1 (MQ=255) gCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGc < 1:3236042/71‑1 (MQ=255) cATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACT‑TATAAACg < 1:940667/63‑1 (MQ=255) atgaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTc > 1:2468357/1‑71 (MQ=255) tgaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATaaa < 1:1822062/60‑1 (MQ=255) tgaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATaaa < 1:1151140/60‑1 (MQ=255) tgaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAAc > 1:3709774/1‑61 (MQ=255) tgaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAAc > 1:1044379/1‑61 (MQ=255) tgaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAAc > 1:2762257/1‑61 (MQ=255) tgaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAAc > 1:3006113/1‑61 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATaaa > 1:2651301/1‑58 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATaaa > 1:2425387/1‑58 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGaa > 1:653135/1‑62 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGaa > 1:2971357/1‑62 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGaa > 1:2278498/1‑62 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGaa > 1:658727/1‑62 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCt > 1:115495/1‑67 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCt > 1:2849848/1‑67 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCt > 1:1131158/1‑67 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCt > 1:154728/1‑67 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCt > 1:1467176/1‑67 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGa < 1:96991/71‑1 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGa < 1:4212447/71‑1 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGAAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGa > 1:175475/1‑71 (MQ=255) aatgaTAGGCGCAAAGCTGGCTAAAGTCGAAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGa > 1:426230/1‑71 (MQ=255) atgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGa < 1:3936041/70‑1 (MQ=255) atgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGa < 1:3729185/70‑1 (MQ=255) aTAGGCGCAAAGCTGGCTAAAGTCGAAGGCAACAt > 1:2634318/1‑35 (MQ=255) aGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATa > 1:1255395/1‑67 (MQ=255) aGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATa > 1:4594041/1‑67 (MQ=255) aGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATa > 1:4620605/1‑67 (MQ=255) gcgcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACg > 1:3385333/1‑71 (MQ=255) cgcTAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCa < 1:2239971/40‑1 (MQ=255) gcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACg > 1:328732/1‑69 (MQ=255) cAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACgg < 1:961631/69‑1 (MQ=255) aaGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGaa > 1:2845875/1‑69 (MQ=255) aaGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGaa > 1:2895945/1‑69 (MQ=255) aaGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGaa > 1:4242256/1‑69 (MQ=255) aGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATc > 1:362471/1‑70 (MQ=255) aGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATc > 1:4705283/1‑70 (MQ=255) ggCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAgg > 1:991293/1‑71 (MQ=255) gCTAAAGTCGGAGTCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACt < 1:2960803/56‑1 (MQ=255) gCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACt < 1:3935217/56‑1 (MQ=255) gCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGt > 1:2608845/1‑71 (MQ=255) cTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTc > 1:1765041/1‑48 (MQ=255) cTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGtt < 1:1924054/71‑1 (MQ=255) cTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGtt < 1:2350739/71‑1 (MQ=255) cTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGtt < 1:830781/71‑1 (MQ=255) tAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGttt > 1:2553096/1‑71 (MQ=255) tAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGttt > 1:386268/1‑71 (MQ=255) tAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGttt > 1:3477987/1‑71 (MQ=255) tAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGttt > 1:3248760/1‑71 (MQ=255) tAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGtt > 1:1984002/1‑70 (MQ=255) aGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCaaa > 1:3037997/1‑63 (MQ=255) gTCGAAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGtt < 1:669005/66‑1 (MQ=255) | GCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTT > Exported/139266‑139397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |