| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,483,542 | C→T | 8.0% | A157V (GCA→GTA) | glpC → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,483,542 | 0 | C | T | 8.0% | 241.2 / 7.7 | 75 | A157V (GCA→GTA) | glpC | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), small subunit |
| Reads supporting (aligned to +/- strand): ref base C (43/26); new base T (2/4); total (45/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAA > Exported/1483473‑1483611 | gccgcACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCagcaagca < 1:1244567/71‑1 (MQ=255)gccgcACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCagcaagc < 1:687158/70‑1 (MQ=255) ccgcACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGcac < 1:398424/71‑1 (MQ=255) ccgcACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGcac < 1:1742599/71‑1 (MQ=255) gcACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACaa > 1:3071693/1‑71 (MQ=255) cGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAatat < 1:25038/71‑1 (MQ=255) cTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGcaca < 1:4678718/65‑1 (MQ=255) cTACCGAAATACTCCTTCGCCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGcaca < 1:3821161/65‑1 (MQ=255) ccGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAg < 1:2471162/70‑1 (MQ=255) ccGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAg < 1:1256645/70‑1 (MQ=255) ccGAAATACTCCTTCGGCACGTTCCGTCGCTGGAATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAg < 1:410996/70‑1 (MQ=255) cGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGa > 1:4360295/1‑70 (MQ=255) cGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGAc > 1:2135966/1‑71 (MQ=255) cGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGAc > 1:1060692/1‑71 (MQ=255) gAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGAc > 1:387685/1‑70 (MQ=255) aTACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:3197257/1‑64 (MQ=255) aTACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:693660/1‑64 (MQ=255) aTACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAgg < 1:1043084/71‑1 (MQ=255) tACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGt < 1:1723052/71‑1 (MQ=255) aCTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGt > 1:3534161/1‑70 (MQ=255) aCTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTc > 1:2743533/1‑71 (MQ=255) cTCCTTCGGCACGTTCCGTCGCTGGTATCTCAGCGTGGCGGCTCAGCAAGCACAata > 1:3102550/1‑57 (MQ=255) cTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCg > 1:633171/1‑71 (MQ=255) cTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCg > 1:1084461/1‑71 (MQ=255) cTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCg > 1:4208212/1‑71 (MQ=255) ccTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGTACAATATAAAGACCAGGTCGct > 1:623817/1‑71 (MQ=255) cGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGa > 1:3223344/1‑57 (MQ=255) cGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttct > 1:3489810/1‑71 (MQ=255) cGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttct > 1:3393063/1‑71 (MQ=255) cGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGcttt < 1:1112368/69‑1 (MQ=255) cGGCACGTTCAGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:3756870/1‑55 (MQ=255) ggCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttctt > 1:4311429/1‑71 (MQ=255) ggCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttct < 1:1478832/70‑1 (MQ=255) ggCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttct < 1:3740/70‑1 (MQ=255) ggCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttct < 1:1727517/70‑1 (MQ=255) gCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttcttt < 1:3659575/71‑1 (MQ=255) cACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttcttt < 1:792320/70‑1 (MQ=255) aCGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCa > 1:2489907/1‑71 (MQ=255) tCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttctt > 1:65847/1‑64 (MQ=255) ccGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGTACAATATAAAGACCAGGTCGCTTTCTTTCACGgct > 1:1036322/1‑71 (MQ=255) ccGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCtttctttc > 1:1822782/1‑65 (MQ=255) ccGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCtttctttc > 1:3724730/1‑65 (MQ=255) gTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGgctgc > 1:3122359/1‑71 (MQ=255) tCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGTACAATATAAAGACCAGGTCGCTTTCTTTCACGgctgct < 1:4579602/71‑1 (MQ=255) tCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGTACAATATAAAGACCAGGTCGCTTTCTTTCACGgctgct < 1:2984520/71‑1 (MQ=255) tCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGgctgct < 1:3294185/71‑1 (MQ=255) gCTGGTATCGCAGCGTGGCGGCTCAGCAAGTACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTc < 1:1609378/71‑1 (MQ=255) gCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCa > 1:1770404/1‑61 (MQ=255) gCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCa > 1:3591853/1‑61 (MQ=255) gCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCa > 1:4066105/1‑61 (MQ=255) gCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTc < 1:222343/71‑1 (MQ=255) tGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGct < 1:812950/51‑1 (MQ=255) ggTATCGCAGCGTGGCGGCTCAGCAAGCACAAtata < 1:2340076/36‑1 (MQ=255) ggTATCGCAGCGTGGCGGCTCAGCAAGCACAAtata < 1:4388079/36‑1 (MQ=255) ggTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:348938/1‑38 (MQ=255) ggTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:2287816/1‑38 (MQ=255) ggTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:2325983/1‑38 (MQ=255) ggTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:2917697/1‑38 (MQ=255) ggTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:1720563/1‑38 (MQ=255) ggTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:4152106/1‑38 (MQ=255) ggTATCGCAGCGTGGCGGCTCAGCAAGCACAATATaaa > 1:4090618/1‑38 (MQ=255) gTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGtt < 1:4157074/70‑1 (MQ=255) tCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCa < 1:3831426/39‑1 (MQ=255) tCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCa < 1:2253810/39‑1 (MQ=255) tCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCa < 1:3192249/39‑1 (MQ=255) tCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctt > 1:3827673/1‑47 (MQ=255) tCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACgg > 1:3675465/1‑57 (MQ=255) tCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCg < 1:4234017/65‑1 (MQ=255) tCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAAc > 1:208737/1‑70 (MQ=255) tCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAAc > 1:2060393/1‑70 (MQ=255) tCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAAc > 1:503463/1‑70 (MQ=255) cAGCGTGGCGGCTCAGCAAGTACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACa < 1:1082790/71‑1 (MQ=255) gTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACaa > 1:1874355/1‑68 (MQ=255) tGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttctt > 1:3482987/1‑43 (MQ=255) tGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCtttctttc > 1:2247712/1‑45 (MQ=255) cggcTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCg > 1:2737852/1‑71 (MQ=255) cggcTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCg < 1:1047742/71‑1 (MQ=255) cTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAg < 1:2454239/71‑1 (MQ=255) cTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAg < 1:1903853/71‑1 (MQ=255) tCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCAc < 1:2484089/40‑1 (MQ=255) tCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGt > 1:2581143/1‑71 (MQ=255) agcaagtaCAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGtt < 1:243976/70‑1 (MQ=255) gcaagcaCAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCg < 1:423185/47‑1 (MQ=255) gcaagcaCAATATAAAGACCAGGTCGCTTTCTTTCACGGCCGCTTCGTTAACTACAACCATCCGc < 1:1125291/65‑1 (MQ=255) gcaCAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTaa < 1:1497171/71‑1 (MQ=255) | GCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAA > Exported/1483473‑1483611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |