Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,694,168 G→C 7.2% P17R (CCG→CGG)  iscS ← cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,694,1680GC 7.2% 232.7 / 6.2 73P17R (CCG→CGG) iscScysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (36/31);  new base C (3/2);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

GAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTC  >  Exported/1694099‑1694231
                                                                      |                                                               
gaACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCgg                                                                <  1:458646/71‑1 (MQ=255)
  aCGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCggg                                                               <  1:2104086/70‑1 (MQ=255)
   cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTc                                                             >  1:625489/1‑71 (MQ=255)
   cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTc                                                             <  1:1958131/71‑1 (MQ=255)
   cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTc                                                             >  1:786568/1‑71 (MQ=255)
      gAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCAc                                                          <  1:167116/71‑1 (MQ=255)
      gAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCAc                                                          <  1:4295352/71‑1 (MQ=255)
             ggTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTg                                                   >  1:987110/1‑71 (MQ=255)
               ttACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGt                                                 <  1:3312584/71‑1 (MQ=255)
                 aCCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGtt                                                >  1:557156/1‑70 (MQ=255)
                 aCCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTg                                               >  1:2765422/1‑71 (MQ=255)
                  ccAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGc                                              >  1:2876430/1‑71 (MQ=255)
                  ccAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGc                                              >  1:144185/1‑71 (MQ=255)
                   cAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             <  1:1483016/71‑1 (MQ=255)
                   cAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             <  1:3036191/71‑1 (MQ=255)
                   cAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             <  1:693309/71‑1 (MQ=255)
                   cAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             <  1:4684166/71‑1 (MQ=255)
                     aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTgg                                                  >  1:2568256/1‑64 (MQ=255)
                     aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             >  1:2262037/1‑69 (MQ=255)
                     aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             >  1:1885585/1‑69 (MQ=255)
                     aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             >  1:1376285/1‑69 (MQ=255)
                     aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             >  1:3713322/1‑69 (MQ=255)
                     aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             >  1:2843325/1‑69 (MQ=255)
                     aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             >  1:2421011/1‑69 (MQ=255)
                             gTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCga                                   <  1:949945/71‑1 (MQ=255)
                             gTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCga                                   <  1:2664001/71‑1 (MQ=255)
                              tCCATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCcgcg                                                     <  1:348113/53‑1 (MQ=17)
                              tCCATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCcgcg                                                     <  1:1324200/53‑1 (MQ=17)
                                cATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCCGCGTg                                                   >  1:4303097/1‑53 (MQ=17)
                                cATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCCGCGTg                                                   >  1:3347546/1‑53 (MQ=17)
                                cATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCCGCGTg                                                   >  1:3992115/1‑53 (MQ=17)
                                cATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCAGGTGGACCCGCGTg                                                   >  1:454780/1‑53 (MQ=18)
                                cATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGa                                           >  1:2123433/1‑60 (MQ=255)
                                cATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGa                                           >  1:3993151/1‑60 (MQ=255)
                                 aTCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCg                                                     <  1:1123912/49‑1 (MQ=255)
                                 aTCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCg                                                     <  1:2484044/49‑1 (MQ=255)
                                 aTCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCg                                                     <  1:2066527/49‑1 (MQ=255)
                                  tCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATaa                              <  1:1300003/71‑1 (MQ=255)
                                  tCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATa                               >  1:3073359/1‑70 (MQ=255)
                                    gTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGt                                                    >  1:1956745/1‑47 (MQ=255)
                                    gTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATaa                              >  1:351449/1‑69 (MQ=255)
                                    gTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt                            <  1:2167152/71‑1 (MQ=255)
                                    gTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt                            <  1:3000198/71‑1 (MQ=255)
                                    gTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt                            <  1:1932629/71‑1 (MQ=255)
                                    gTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt                            >  1:3092883/1‑71 (MQ=255)
                                    gTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt                            <  1:4159018/71‑1 (MQ=255)
                                    gTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTACACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt                            <  1:3825822/71‑1 (MQ=255)
                                       aTAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCgg                         >  1:1994963/1‑71 (MQ=255)
                                       aTAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCgg                         >  1:4112090/1‑71 (MQ=255)
                                        tAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGt                        <  1:1217204/71‑1 (MQ=255)
                                        tAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGt                        <  1:3757865/71‑1 (MQ=255)
                                        tAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGt                        <  1:3397106/71‑1 (MQ=255)
                                         aaaCTGCATCATTTTCTCGGCAACACGCGGGTCCa                                                           >  1:1523454/1‑35 (MQ=255)
                                         aaaCTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTgg                                                  >  1:1478470/1‑44 (MQ=255)
                                         aaaCTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTgg                                                  >  1:3759844/1‑44 (MQ=255)
                                         aaaCTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTgg                                                  >  1:2911189/1‑44 (MQ=255)
                                         aaaCTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTgg                                                  >  1:391077/1‑44 (MQ=255)
                                          aaCTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTgg                                                  >  1:1411993/1‑43 (MQ=255)
                                          aaCTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTgg                                                  >  1:438666/1‑43 (MQ=255)
                                          aaCTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTaa                      <  1:3434808/71‑1 (MQ=255)
                                            cTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGt                                                 >  1:1485243/1‑42 (MQ=255)
                                            cTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGt                                                 >  1:694269/1‑42 (MQ=255)
                                              gCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCg                                             <  1:787599/44‑1 (MQ=255)
                                              gCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt                            <  1:3041108/61‑1 (MQ=255)
                                               catcatTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                   <  1:4165728/69‑1 (MQ=255)
                                               catcatTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa                 <  1:2188294/71‑1 (MQ=255)
                                                   atTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGc             <  1:1935625/71‑1 (MQ=255)
                                                      ttCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGagtag                                       >  1:3720751/1‑42 (MQ=255)
                                                      ttCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctc           >  1:3169639/1‑70 (MQ=255)
                                                        ctcGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCta         <  1:772784/70‑1 (MQ=255)
                                                           ggCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGc             <  1:1556782/63‑1 (MQ=255)
                                                           ggCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCtata       <  1:1805488/69‑1 (MQ=255)
                                                           ggCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATaaa     >  1:2502021/1‑71 (MQ=255)
                                                              aaCACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTg              >  1:989232/1‑59 (MQ=255)
                                                              aaCACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTc  >  1:3567120/1‑71 (MQ=255)
                                                                   cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                   <  1:582013/50‑1 (MQ=255)
                                                                   cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                   <  1:2022115/50‑1 (MQ=255)
                                                                   cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                   <  1:3073211/50‑1 (MQ=255)
                                                                   cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                   <  1:3297253/50‑1 (MQ=255)
                                                                   cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                   <  1:1676248/50‑1 (MQ=255)
                                                                   cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                   <  1:4226358/50‑1 (MQ=255)
                                                                   cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa                 >  1:2303063/1‑52 (MQ=255)
                                                                    gcgGGTCCACCGGCGTTGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa                 <  1:1572957/51‑1 (MQ=255)
                                                                    gcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa                 <  1:526466/51‑1 (MQ=255)
                                                                    gcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa                 <  1:2615394/51‑1 (MQ=255)
                                                                    gcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa                 <  1:1489171/51‑1 (MQ=255)
                                                                    gcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa                 <  1:3392036/51‑1 (MQ=255)
                                                                      |                                                               
GAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAACACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTC  >  Exported/1694099‑1694231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: