| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,694,172 | C→G | 7.3% | D16H (GAC→CAC) | iscS ← | cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,694,172 | 0 | C | G | 7.3% | 263.0 / 6.9 | 82 | D16H (GAC→CAC) | iscS | cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (35/40); new base G (4/2); total (39/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTC > Exported/1694102‑1694231 | cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTc > 1:786568/1‑71 (MQ=255)cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTc < 1:1958131/71‑1 (MQ=255)cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTc > 1:625489/1‑71 (MQ=255) gAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCAc < 1:167116/71‑1 (MQ=255) gAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCAc < 1:4295352/71‑1 (MQ=255) ggTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTg > 1:987110/1‑71 (MQ=255) ttACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGt < 1:3312584/71‑1 (MQ=255) aCCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGtt > 1:557156/1‑70 (MQ=255) aCCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTg > 1:2765422/1‑71 (MQ=255) ccAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGc > 1:2876430/1‑71 (MQ=255) ccAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGc > 1:144185/1‑71 (MQ=255) cAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg < 1:1483016/71‑1 (MQ=255) cAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg < 1:3036191/71‑1 (MQ=255) cAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg < 1:4684166/71‑1 (MQ=255) cAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg < 1:693309/71‑1 (MQ=255) aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTgg > 1:2568256/1‑64 (MQ=255) aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg > 1:2262037/1‑69 (MQ=255) aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg > 1:2843325/1‑69 (MQ=255) aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg > 1:2421011/1‑69 (MQ=255) aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg > 1:1885585/1‑69 (MQ=255) aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg > 1:3713322/1‑69 (MQ=255) aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg > 1:1376285/1‑69 (MQ=255) gTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCga < 1:949945/71‑1 (MQ=255) gTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCga < 1:2664001/71‑1 (MQ=255) tCCATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCcgcg < 1:348113/53‑1 (MQ=17) tCCATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCcgcg < 1:1324200/53‑1 (MQ=17) cATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCCGCGTg > 1:4303097/1‑53 (MQ=17) cATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCCGCGTg > 1:3992115/1‑53 (MQ=17) cATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCCGGTGGACCCGCGTg > 1:3347546/1‑53 (MQ=17) cATCGTCATAAACTGCATCATTTTCTCGGCAACCACGCAGGTGGACCCGCGTg > 1:454780/1‑53 (MQ=18) cATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGa > 1:2123433/1‑60 (MQ=255) cATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGa > 1:3993151/1‑60 (MQ=255) aTCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCg < 1:2484044/49‑1 (MQ=255) aTCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCg < 1:2066527/49‑1 (MQ=255) aTCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCg < 1:1123912/49‑1 (MQ=255) tCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATaa < 1:1300003/71‑1 (MQ=255) tCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATa > 1:3073359/1‑70 (MQ=255) gTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGt > 1:1956745/1‑47 (MQ=255) gTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATaa > 1:351449/1‑69 (MQ=255) gTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt < 1:2167152/71‑1 (MQ=255) gTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt < 1:4159018/71‑1 (MQ=255) gTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt > 1:3092883/1‑71 (MQ=255) gTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt < 1:3000198/71‑1 (MQ=255) gTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt < 1:1932629/71‑1 (MQ=255) gTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTACACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt < 1:3825822/71‑1 (MQ=255) aTAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCgg > 1:4112090/1‑71 (MQ=255) aTAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCgg > 1:1994963/1‑71 (MQ=255) tAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGt < 1:3757865/71‑1 (MQ=255) tAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGt < 1:3397106/71‑1 (MQ=255) tAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGt < 1:1217204/71‑1 (MQ=255) aaaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCa > 1:1523454/1‑35 (MQ=255) aaaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTgg > 1:2911189/1‑44 (MQ=255) aaaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTgg > 1:1478470/1‑44 (MQ=255) aaaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTgg > 1:3759844/1‑44 (MQ=255) aaaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTgg > 1:391077/1‑44 (MQ=255) aaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTgg > 1:438666/1‑43 (MQ=255) aaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTgg > 1:1411993/1‑43 (MQ=255) aaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTaa < 1:3434808/71‑1 (MQ=255) cTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGt > 1:694269/1‑42 (MQ=255) cTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGt > 1:1485243/1‑42 (MQ=255) gCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCg < 1:787599/44‑1 (MQ=255) gCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAAt < 1:3041108/61‑1 (MQ=255) catcatTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt < 1:4165728/69‑1 (MQ=255) catcatTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa < 1:2188294/71‑1 (MQ=255) atTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGc < 1:1935625/71‑1 (MQ=255) ttCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGagtag > 1:3720751/1‑42 (MQ=255) ttCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctc > 1:3169639/1‑70 (MQ=255) ctcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCta < 1:772784/70‑1 (MQ=255) ggCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGc < 1:1556782/63‑1 (MQ=255) ggCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCtata < 1:1805488/69‑1 (MQ=255) ggCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATaaa > 1:2502021/1‑71 (MQ=255) aa‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTg > 1:989232/1‑59 (MQ=255) aa‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTc > 1:3567120/1‑71 (MQ=255) cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt < 1:582013/50‑1 (MQ=255) cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt < 1:2022115/50‑1 (MQ=255) cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt < 1:3073211/50‑1 (MQ=255) cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt < 1:4226358/50‑1 (MQ=255) cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt < 1:1676248/50‑1 (MQ=255) cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt < 1:3297253/50‑1 (MQ=255) cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa > 1:2303063/1‑52 (MQ=255) gcgGGTCCACCGGCGTTGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa < 1:1572957/51‑1 (MQ=255) gcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa < 1:526466/51‑1 (MQ=255) gcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa < 1:2615394/51‑1 (MQ=255) gcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa < 1:1489171/51‑1 (MQ=255) gcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCa < 1:3392036/51‑1 (MQ=255) | CGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTC > Exported/1694102‑1694231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |