| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,706,900 | T→C | 9.9% | I159V (ATT→GTT) | yphB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,706,900 | 0 | T | C | 9.9% | 267.7 / 17.3 | 91 | I159V (ATT→GTT) | yphB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (51/31); new base C (5/4); total (56/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
GCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTT > Exported/1706834‑1706968 | gctCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCcgc > 1:215346/1‑71 (MQ=255) ctCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCcgc < 1:2704092/70‑1 (MQ=255) tCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAACCCGCGt > 1:199702/1‑71 (MQ=255) tCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAACCCGCGt > 1:2868304/1‑71 (MQ=255) gCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAACCCGCGtt < 1:4121172/70‑1 (MQ=255) aaCTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTgcgg > 1:1275226/1‑71 (MQ=255) aaCTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTgcgg > 1:1678297/1‑71 (MQ=255) aaCTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTgcgg > 1:4486607/1‑71 (MQ=255) aCCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGAc > 1:3032225/1‑70 (MQ=255) gccagccaCTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTG‑GGCGACAACgg > 1:554484/1‑70 (MQ=255) gccagccaCTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAACCCGCGTTTGCGGCGACAACg > 1:1660497/1‑70 (MQ=255) agccaCTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGaa > 1:2829318/1‑70 (MQ=255) agccaCTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGaa > 1:4608772/1‑70 (MQ=255) gcTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGaaaa > 1:2968423/2‑68 (MQ=255) aCTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGa > 1:4630636/1‑65 (MQ=255) tGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGtt < 1:661234/48‑1 (MQ=255) tGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGtt < 1:1897492/48‑1 (MQ=255) tGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGtt < 1:510840/48‑1 (MQ=255) gCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGaaaa > 1:807323/1‑65 (MQ=255) gCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAgg > 1:1563595/1‑70 (MQ=255) gCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGa > 1:3169644/1‑71 (MQ=255) gCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGa > 1:2412551/1‑71 (MQ=255) gCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGa > 1:2590193/1‑71 (MQ=255) gCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAACCCGCGTTTGCGGCGACAACGGAAAATAAGGa > 1:2593519/1‑71 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATc > 1:3057353/1‑37 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATc > 1:2054887/1‑37 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATc > 1:2245645/1‑37 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATc > 1:2413661/1‑37 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATc > 1:2507033/1‑37 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGt > 1:2352690/1‑42 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAAt > 1:3533829/1‑62 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAAt > 1:4703444/1‑62 (MQ=255) cGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGc > 1:1560706/1‑69 (MQ=255) gCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAg > 1:3215483/1‑71 (MQ=255) cTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGaaaa < 1:988864/58‑1 (MQ=255) cTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGc < 1:2254585/71‑1 (MQ=255) cTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGc < 1:1457712/71‑1 (MQ=255) ccagccagTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCg > 1:1406212/1‑71 (MQ=255) cagccagTAACCGCTCGCCTGTGCCTGAATCcgcg < 1:854962/35‑1 (MQ=255) cagccagTAACCGCTCGCCTGTGCCTGAATCcgcg < 1:2709315/35‑1 (MQ=255) cagccagTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGcc > 1:4274153/1‑65 (MQ=255) cagccagTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGcc < 1:2980717/69‑1 (MQ=255) gccagTAACCGCTCGCCTGTGCCTGAATCCGGGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTa > 1:3768907/1‑71 (MQ=255) gccagTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTa > 1:982249/1‑71 (MQ=255) agTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTAcc > 1:1344322/1‑70 (MQ=255) agTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTAcc > 1:260000/1‑70 (MQ=255) gTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGaaaa < 1:248166/49‑1 (MQ=255) gTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAAt < 1:3984798/50‑1 (MQ=255) gTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCaa < 1:3780981/71‑1 (MQ=255) tAACCGCTCGCCTGTGCCTTAATCCGCGTTTGCCGCGACAACGGAAAATAAGGATGCCAGCCGGTACCaaa > 1:738254/1‑71 (MQ=255) tAACCGCTCGCCTGTGCCTGAATCCGCGTTTgcgg > 1:507738/1‑35 (MQ=255) tAACCGCTCGCCTGTGCCTGAATCCGCGTTTgcgg > 1:2412214/1‑35 (MQ=255) tAACCGCTCGCCTGTGCCTGAATCCGCGTTTgcgg > 1:2033558/1‑35 (MQ=255) tAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTAc < 1:1093304/67‑1 (MQ=255) aCCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATg < 1:956958/71‑1 (MQ=255) aCCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATg < 1:3654685/71‑1 (MQ=255) aCCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATg < 1:770478/71‑1 (MQ=255) aCCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATg < 1:2559962/71‑1 (MQ=255) ccGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATgg < 1:1869404/71‑1 (MQ=255) cGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGc < 1:2036074/71‑1 (MQ=255) gCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATgg > 1:3631662/1‑69 (MQ=255) ccTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtc > 1:3581153/1‑71 (MQ=255) cTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtct > 1:561134/1‑71 (MQ=255) cTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtct > 1:4757015/1‑71 (MQ=255) cTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtct > 1:4627965/1‑71 (MQ=255) cTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtct > 1:3396580/1‑71 (MQ=255) cTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtc > 1:1157652/1‑70 (MQ=255) tgtgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGcc < 1:194277/50‑1 (MQ=255) tgtgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGcc < 1:2381412/50‑1 (MQ=255) tgtgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCgg > 1:3200658/1‑52 (MQ=255) tgtgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCaaa > 1:3596549/1‑59 (MQ=255) tgtgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCaaa > 1:2295792/1‑59 (MQ=255) tgtgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtctc > 1:3599168/1‑71 (MQ=255) tgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTg < 1:4243830/71‑1 (MQ=255) tgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTg < 1:1843206/71‑1 (MQ=255) tgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTg < 1:2041965/71‑1 (MQ=255) tgCCTGAACCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtctc < 1:624312/69‑1 (MQ=255) tgCCTGAACCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtctc < 1:894824/69‑1 (MQ=255) tgCCTGAACCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtctc < 1:3619560/69‑1 (MQ=255) ccTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGc > 1:4553127/1‑62 (MQ=255) ccTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGc > 1:492261/1‑62 (MQ=255) ccTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGcc > 1:4661523/1‑71 (MQ=255) ccTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGcc < 1:3465243/71‑1 (MQ=255) ccTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGcc < 1:4378063/71‑1 (MQ=255) gAACCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCt > 1:3925332/1‑71 (MQ=255) aaTCCGCGTTTGCGGCGACAACGGAAAATAAGGATGc < 1:3739196/37‑1 (MQ=255) aaTCCGCGTTTGCGGCGACAACGGAAAATAAGGATGc < 1:530884/37‑1 (MQ=255) aaTCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGc > 1:3592657/1‑55 (MQ=255) aaTCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCATCGTCTCTGCCCCtt < 1:189340/71‑1 (MQ=255) aaTCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCtt > 1:1850411/1‑71 (MQ=255) aaTCCGAGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCtt < 1:2339288/71‑1 (MQ=255) tCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCa > 1:842557/1‑37 (MQ=255) tCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCa > 1:933414/1‑37 (MQ=255) tCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCa > 1:2645901/1‑37 (MQ=255) tCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCa < 1:2494186/47‑1 (MQ=255) tCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATg < 1:4354659/51‑1 (MQ=255) tCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATg < 1:4349426/51‑1 (MQ=255) tCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATg < 1:3075662/51‑1 (MQ=255) tCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtctc > 1:1713028/1‑61 (MQ=255) | GCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTT > Exported/1706834‑1706968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |