| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,744,552 | G→A | 9.6% | V483I (GTC→ATC) | nadB → | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,744,552 | 0 | G | A | 9.6% | 252.4 / 14.3 | 83 | V483I (GTC→ATC) | nadB | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| Reads supporting (aligned to +/- strand): ref base G (37/38); new base A (3/5); total (40/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
CTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAATGAT > Exported/1744483‑1744622 | cTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCcgcg < 1:3545288/70‑1 (MQ=255) gAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGtctc < 1:2667595/71‑1 (MQ=255) cgcCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAaata < 1:4365326/71‑1 (MQ=255) cgcCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAaata < 1:4338517/71‑1 (MQ=255) cgcCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAaat > 1:3927519/1‑70 (MQ=255) cggcggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGc < 1:1862292/70‑1 (MQ=255) gcggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTgg < 1:3686659/71‑1 (MQ=255) ggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg < 1:2432712/71‑1 (MQ=255) ggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg > 1:2201812/1‑71 (MQ=255) aTAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCt > 1:2743172/1‑71 (MQ=255) aCCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCg > 1:135996/1‑71 (MQ=255) cATGCTCCAACATGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAaata < 1:1667534/53‑1 (MQ=255) cATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTa < 1:4629649/71‑1 (MQ=255) cATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTa < 1:2343477/71‑1 (MQ=255) tGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAaataa < 1:2747344/52‑1 (MQ=255) tGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAaataa < 1:1336414/52‑1 (MQ=255) tGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTaa > 1:2879366/1‑70 (MQ=255) cTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCt < 1:1679397/71‑1 (MQ=255) cTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCt < 1:3585149/71‑1 (MQ=255) ccAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTgg < 1:461718/56‑1 (MQ=255) ccAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTgg < 1:4314479/56‑1 (MQ=255) ccAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTgg < 1:2756937/56‑1 (MQ=255) caacaaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGa < 1:4135672/56‑1 (MQ=255) aacaaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTa > 1:3001145/1‑71 (MQ=255) acaaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTg > 1:1702257/1‑58 (MQ=255) acaaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGc < 1:3146866/59‑1 (MQ=255) acaaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGc < 1:2680003/59‑1 (MQ=255) acaaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTa < 1:1016094/62‑1 (MQ=255) acaaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTa > 1:4345743/1‑70 (MQ=255) caaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGc > 1:75432/1‑58 (MQ=255) caaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGc > 1:3494108/1‑58 (MQ=255) aaGAAATAGACGAATATTACGCCCATTTCCGGGTCTCAAATAATTTGCTgg < 1:170610/51‑1 (MQ=255) aaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAaataat > 1:3054307/1‑44 (MQ=255) aaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAaataat > 1:4081394/1‑44 (MQ=255) aaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAAt > 1:2656959/1‑62 (MQ=255) aaGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAAt > 1:1887500/1‑62 (MQ=255) aaaTAGACGAATATTACGCCCATTTCCGCGTCTCAaataa < 1:2009218/40‑1 (MQ=255) aaTAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTg < 1:3982576/71‑1 (MQ=255) tAGACGAATATTACGCCCATTTCCGCGTCTCAaataa < 1:3563472/37‑1 (MQ=255) tAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGcc < 1:4456600/71‑1 (MQ=255) aGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTaa < 1:1181879/54‑1 (MQ=255) aGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTaa < 1:1358811/54‑1 (MQ=255) gACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGa > 1:2914751/1‑44 (MQ=255) gACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGa > 1:2858395/1‑44 (MQ=255) gACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGa > 1:4714672/1‑44 (MQ=255) gACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGa > 1:4720018/1‑44 (MQ=255) gACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGa > 1:4758084/1‑44 (MQ=255) gACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGa > 1:3987544/1‑44 (MQ=255) gACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGa > 1:4613896/1‑44 (MQ=255) aCGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTgg < 1:1313126/42‑1 (MQ=255) aCGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTgg < 1:4563857/42‑1 (MQ=255) aCGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTgg < 1:43352/42‑1 (MQ=255) aCGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTgg < 1:3668239/42‑1 (MQ=255) aCGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGa < 1:4744362/70‑1 (MQ=255) aCGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAg < 1:1383472/71‑1 (MQ=255) aCGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAg < 1:2585839/71‑1 (MQ=255) aCGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAg < 1:4474749/71‑1 (MQ=255) aaTATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCt < 1:67231/52‑1 (MQ=255) atatTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGc > 1:2859840/1‑44 (MQ=255) atatTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGa > 1:364342/1‑71 (MQ=255) tatTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGAt < 1:4322706/71‑1 (MQ=255) tatTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGAt < 1:2674126/71‑1 (MQ=255) ttACGCCCATTTCCGCGTCTGAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGAtt < 1:1492451/70‑1 (MQ=255) ttACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGAtt < 1:772204/70‑1 (MQ=255) ttACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTg > 1:4410387/1‑71 (MQ=255) aCGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGt > 1:1124596/1‑70 (MQ=255) cccATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGc < 1:1447536/71‑1 (MQ=255) aTTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt > 1:4010825/1‑71 (MQ=255) tttCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTg > 1:1221519/1‑62 (MQ=255) tttCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTg > 1:3462205/1‑62 (MQ=255) tttCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgtg > 1:1695472/1‑71 (MQ=255) tttCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGACGAGTTGATTg > 1:277216/1‑62 (MQ=255) tttCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGtt > 1:3722831/1‑64 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTAGAGGTTGCCGAGTTGATTGTTCGCtg > 1:771931/1‑67 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGcc > 1:512319/1‑50 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt > 1:4256417/1‑68 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt > 1:4023000/1‑68 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt > 1:4516961/1‑68 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt > 1:1360636/1‑68 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGc > 1:83305/1‑70 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGc > 1:418593/1‑70 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGc > 1:4411267/1‑70 (MQ=255) ccGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTTTGCa < 1:4682668/70‑1 (MQ=255) ccGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCa < 1:2454468/70‑1 (MQ=255) cgcgTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGc < 1:1377786/68‑1 (MQ=255) gTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAatgat > 1:2975342/1‑71 (MQ=255) | CTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAATGAT > Exported/1744483‑1744622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |