Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,763,558 G→C 10.2% noncoding (286/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,763,5580GC10.2% 505.5 / 39.5 176noncoding (286/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (76/82);  new base C (12/6);  total (88/88)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.36e-01

Only 100 of 183 total aligned reads displayed.

GGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAG  >  Exported/1763502‑1763627
                                                        |                                                                     
ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTg                                                         >  1:3628801/1‑71 (MQ=32)
ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCtt                                                          <  1:1004722/70‑1 (MQ=14)
ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTg                                                         >  1:2319412/1‑71 (MQ=17)
ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTg                                                         >  1:1921084/1‑71 (MQ=17)
ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTg                                                         >  1:1858234/1‑71 (MQ=17)
  aGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGt                                                       <  1:4079601/71‑1 (MQ=34)
      tGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGc                                                   >  1:3367420/1‑71 (MQ=21)
      tGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGc                                                   >  1:4731660/1‑71 (MQ=21)
        gACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAg                                                    <  1:3369704/68‑1 (MQ=35)
         aCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                                >  1:2474083/1‑71 (MQ=34)
          ccGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGtt                                               <  1:428441/71‑1 (MQ=21)
          ccGTGTCTCAGGTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGtt                                               <  1:3223644/71‑1 (MQ=21)
           cGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTa                                              >  1:3741835/1‑71 (MQ=34)
             tgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAcc                                            >  1:2500466/1‑71 (MQ=34)
             tgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTAc                                             <  1:3859730/70‑1 (MQ=25)
                ctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGCCCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCcca                                         >  1:4619897/1‑71 (MQ=255)
                ctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCcca                                         >  1:798499/1‑71 (MQ=21)
                ctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCcca                                         >  1:4199549/1‑71 (MQ=21)
                ctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCcca                                         >  1:2101466/1‑71 (MQ=21)
                 tcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCtt                                                          <  1:1898411/53‑1 (MQ=31)
                 tcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAc                                             >  1:2042295/1‑66 (MQ=35)
                 tcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGt                                                       >  1:2831627/1‑56 (MQ=255)
                 tcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTAc                                             >  1:3139172/1‑66 (MQ=25)
                   aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTgg                                                        >  1:3943943/1‑53 (MQ=35)
                   aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCccacc                                       >  1:2318077/1‑70 (MQ=35)
                   aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCcca                                         <  1:3678858/68‑1 (MQ=35)
                   aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCcca                                         <  1:3415938/68‑1 (MQ=35)
                   aGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTACCcca                                         <  1:4298232/68‑1 (MQ=11)
                    gTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaa                                     >  1:961651/1‑71 (MQ=38)
                     ttCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                    <  1:891497/71‑1 (MQ=39)
                     ttCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGcc                                                  <  1:1138459/57‑1 (MQ=255)
                     ttCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGcc                                                  <  1:4511650/57‑1 (MQ=255)
                     ttCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGcc                                                  <  1:4560797/57‑1 (MQ=255)
                     ttCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGcc                                                  <  1:4663587/57‑1 (MQ=255)
                      tCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                    >  1:3579975/1‑70 (MQ=255)
                      tCCAGTGTGGCTAGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                    >  1:2180233/1‑70 (MQ=255)
                       ccAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                    >  1:3785609/1‑69 (MQ=40)
                           tgtgGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTAcctc                                          >  1:974829/1‑57 (MQ=11)
                           tgtgGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTAcctc                                          >  1:3933247/1‑57 (MQ=11)
                           tgtgGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTAcctc                                          >  1:930577/1‑57 (MQ=11)
                           tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCtt                                                          <  1:2212591/43‑1 (MQ=32)
                           tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCtt                                                          <  1:3611651/43‑1 (MQ=32)
                           tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCtt                                                          <  1:716173/43‑1 (MQ=32)
                           tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCtt                                                          <  1:1535058/43‑1 (MQ=32)
                           tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                              <  1:3040396/71‑1 (MQ=255)
                           tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                              <  1:561457/71‑1 (MQ=255)
                           tgtgGCTGGGCATCCTCTCAGACCAGCTAGGGATCGTCGCCtt                                                          <  1:1203898/43‑1 (MQ=12)
                            gtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                             >  1:3454117/1‑71 (MQ=255)
                            gtgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                             >  1:1212042/1‑71 (MQ=255)
                             tgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                             <  1:1324711/70‑1 (MQ=255)
                             tgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                             <  1:3389987/70‑1 (MQ=255)
                              ggCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt                            <  1:3099215/70‑1 (MQ=255)
                              ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccac                                        <  1:3971345/58‑1 (MQ=255)
                              ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccac                                        <  1:1067824/58‑1 (MQ=255)
                              ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccac                                        <  1:1644756/58‑1 (MQ=255)
                               gCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTAATca                          <  1:327476/71‑2 (MQ=11)
                               gCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTAATca                          <  1:4369799/71‑2 (MQ=11)
                               gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTAcc                                            >  1:1876064/1‑53 (MQ=255)
                               gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                          <  1:1848359/71‑1 (MQ=255)
                                cTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTg                                                         >  1:4217589/1‑39 (MQ=37)
                                cTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTg                                                         >  1:4061959/1‑39 (MQ=37)
                                 tGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCcca                                         >  1:3643056/1‑54 (MQ=35)
                                 tGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATccc                         <  1:662157/70‑1 (MQ=255)
                                  ggTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                       >  1:2265316/1‑71 (MQ=255)
                                   gTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                             >  1:2485762/1‑64 (MQ=255)
                                   gTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                              <  1:21607/63‑1 (MQ=255)
                                   gTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCa                        <  1:3785622/69‑1 (MQ=255)
                                     cATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCAATCTg                    >  1:1240109/1‑71 (MQ=255)
                                       tCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTggg                  <  1:4446918/71‑1 (MQ=255)
                                         ctctCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAcc                                            >  1:2706952/1‑43 (MQ=38)
                                         ctctCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                              >  1:4642159/1‑57 (MQ=255)
                                         ctctCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                       >  1:1965886/1‑64 (MQ=255)
                                         ctctCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                       >  1:476005/1‑64 (MQ=255)
                                         ctctCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACcaa                                     >  1:133083/1‑50 (MQ=14)
                                         ctctCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaa                                     >  1:387119/1‑50 (MQ=255)
                                           ctcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGcaca              >  1:1543872/1‑71 (MQ=255)
                                            tcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaaca                                   <  1:1699503/49‑1 (MQ=255)
                                            tcAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTa                                              <  1:3178927/38‑1 (MQ=255)
                                              aGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATccga                        >  1:79743/1‑56 (MQ=11)
                                              aGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATccga                        >  1:252049/1‑56 (MQ=11)
                                              aGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATccga                        >  1:3170688/1‑56 (MQ=11)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGtt                                               >  1:2788129/1‑35 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGtt                                               >  1:3767065/1‑35 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGca                >  1:2292478/1‑66 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACAt             <  1:3975296/69‑1 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACAt             <  1:3004847/69‑1 (MQ=255)
                                                  cAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATccc                         <  1:2532926/53‑1 (MQ=255)
                                                  cAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCACCAACAAGCTAATCCCATCTGGGCACATGCGATgg      <  1:4418/71‑1 (MQ=14)
                                                   aGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg      <  1:1185447/71‑1 (MQ=255)
                                                   aGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg      <  1:177761/71‑1 (MQ=255)
                                                   aGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg      <  1:2387595/71‑1 (MQ=255)
                                                   aGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg      <  1:1303607/71‑1 (MQ=255)
                                                      caGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCACCAAGCTAATCCCATCTGGGCACATCCGATGGCaa   <  1:2434602/70‑1 (MQ=255)
                                                      caGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGc     >  1:3312778/2‑69 (MQ=255)
                                                      caGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa   <  1:1202027/70‑1 (MQ=255)
                                                       aGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa   <  1:4175146/70‑1 (MQ=255)
                                                       aGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAag  <  1:4435866/71‑1 (MQ=255)
                                                       aGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAag  <  1:2593443/71‑1 (MQ=255)
                                                        gggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa   <  1:2837172/69‑1 (MQ=255)
                                                        gggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAag  <  1:3984355/70‑1 (MQ=255)
                                                        |                                                                     
GGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAG  >  Exported/1763502‑1763627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: