Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,763,594 A→T 6.5% noncoding (250/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,763,5940AT 6.5% 458.0 / 14.8 150noncoding (250/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (51/89);  new base T (5/5);  total (56/94)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 158 total aligned reads displayed.

TGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACAT  >  Exported/1763529‑1763662
                                                                 |                                                                    
tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                                                                 <  1:3040396/71‑1 (MQ=255)
 gtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                                >  1:3454117/1‑71 (MQ=255)
 gtgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                                >  1:1212042/1‑71 (MQ=255)
  tgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                                <  1:1324711/70‑1 (MQ=255)
  tgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt                                                               <  1:2810103/71‑1 (MQ=255)
  tgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                                <  1:3389987/70‑1 (MQ=255)
  tgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt                                                               >  1:3116563/1‑71 (MQ=255)
  tgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt                                                               >  1:1558553/1‑71 (MQ=255)
    gCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTAATca                                                             <  1:327476/71‑2 (MQ=11)
    gCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTAATca                                                             <  1:1849771/71‑2 (MQ=11)
    gCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACCAACTAGCTAATca                                                             <  1:4369799/71‑2 (MQ=11)
    gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                                                             >  1:2479877/1‑71 (MQ=255)
    gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                                                             <  1:1108347/71‑1 (MQ=255)
      tGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATccc                                                            <  1:662157/70‑1 (MQ=255)
       ggTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                                                          >  1:2265316/1‑71 (MQ=255)
        gTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                                >  1:2485762/1‑64 (MQ=255)
        gTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                                                                 <  1:21607/63‑1 (MQ=255)
        gTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCa                                                           <  1:3785622/69‑1 (MQ=255)
         tCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCa                                                           <  1:386002/68‑1 (MQ=255)
         tCATCCTCTCAGACCAGCCAGGGATCGGCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                                                                 <  1:896226/62‑1 (MQ=255)
          cATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCAATCTg                                                       >  1:1240109/1‑71 (MQ=255)
            tCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTggg                                                     <  1:3827215/71‑1 (MQ=255)
              ctctCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                                                          >  1:1965886/1‑64 (MQ=255)
              ctctCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                                                          >  1:476005/1‑64 (MQ=255)
              ctctCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGc                                                    <  1:4074086/70‑1 (MQ=255)
              ctctCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGc                                                    <  1:4464196/70‑1 (MQ=255)
                ctcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGcaca                                                 >  1:1543872/1‑71 (MQ=255)
                 tcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaaca                                                                      <  1:1699503/49‑1 (MQ=255)
                  cAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATc                                               >  1:414979/1‑71 (MQ=255)
                   aGACCTGCCAGGGATCGTCGCCTTGGTGAGCCGTTTCCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                              <  1:3782029/71‑1 (MQ=14)
                   aGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATccga                                                           >  1:252049/1‑56 (MQ=11)
                   aGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATccga                                                           >  1:2006983/1‑56 (MQ=11)
                   aGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                              >  1:3478404/1‑71 (MQ=255)
                   aGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATc                                               >  1:620793/1‑70 (MQ=255)
                   aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCa                                                           >  1:253790/1‑58 (MQ=255)
                   aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGca                                                   >  1:2292478/1‑66 (MQ=255)
                   aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACAt                                                <  1:3975296/69‑1 (MQ=255)
                   aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACAt                                                <  1:370768/69‑1 (MQ=255)
                   aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                              <  1:3941385/71‑1 (MQ=255)
                   aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                              <  1:2397039/71‑1 (MQ=255)
                       cAGCCAGGGATCGTCGCCTTGGTGAGCCGTTA‑CCCACCAACAAGCTAATCCCATCTGGGCACATGCGATgg                                         <  1:4418/71‑1 (MQ=14)
                        aGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg                                         <  1:1185447/71‑1 (MQ=255)
                        aGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg                                         <  1:1303607/71‑1 (MQ=255)
                        aGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg                                         <  1:177761/71‑1 (MQ=255)
                        aGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg                                         <  1:2387595/71‑1 (MQ=255)
                           caGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCACCAAGCTAATCCCATCTGGGCACATCCGATGGCaa                                      <  1:2434602/70‑1 (MQ=255)
                           caGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa                                      <  1:1202027/70‑1 (MQ=255)
                           caGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa                                      <  1:1801673/70‑1 (MQ=255)
                            aGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAag                                     <  1:4435866/71‑1 (MQ=255)
                            aGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAag                                     <  1:4623328/71‑1 (MQ=255)
                             gggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa                                      <  1:1918382/69‑1 (MQ=255)
                             gggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa                                      <  1:2885663/69‑1 (MQ=255)
                             gggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAag                                     <  1:3984355/70‑1 (MQ=255)
                               gATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAaga                                    >  1:1205961/1‑69 (MQ=255)
                               gATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAaga                                    >  1:2855736/1‑69 (MQ=255)
                                aTCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGc                                 <  1:2377411/71‑1 (MQ=255)
                                aTCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGc                                 <  1:1389903/71‑1 (MQ=255)
                                 tcttcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGc                                 <  1:4286242/67‑1 (MQ=255)
                                 tcgtcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGc                                 <  1:1807675/70‑1 (MQ=255)
                                 tcgtcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGc                                 <  1:3703519/70‑1 (MQ=255)
                                  cgtcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATccc                                                            <  1:4713566/42‑1 (MQ=255)
                                  cgtcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGccc                               >  1:4581910/1‑71 (MQ=255)
                                   gtcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCg                              <  1:2155986/71‑1 (MQ=255)
                                   gtcgCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATCCGACCTAGGCTCATCTGATAGCGTGAGGTCCg                              <  1:3956989/71‑1 (MQ=255)
                                    tcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa                                      <  1:514597/62‑1 (MQ=255)
                                    tcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGa                             <  1:3078324/71‑1 (MQ=255)
                                      gCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGc                                 <  1:297968/65‑1 (MQ=255)
                                      gCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAg                           >  1:4255611/1‑71 (MQ=255)
                                      gCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAg                           <  1:1053618/71‑1 (MQ=255)
                                         ttGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGt                         <  1:596083/70‑1 (MQ=255)
                                          tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAag                                     <  1:4700308/57‑1 (MQ=255)
                                          tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAgg                                  >  1:972280/1‑60 (MQ=255)
                                          tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAgg                                  >  1:711066/1‑60 (MQ=255)
                                          tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAgg                                  >  1:3172866/1‑60 (MQ=255)
                                          tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTcc                       >  1:516459/1‑71 (MQ=255)
                                          tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTcc                       >  1:4708828/1‑71 (MQ=255)
                                           ggTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTccc                      >  1:245894/1‑71 (MQ=39)
                                             tGAGCCGTTTCCCCACCAACAAGCTAATCCCTTCTGGGCACATCCGATGGCAAGAGGCCCGa                             <  1:907629/62‑1 (MQ=255)
                                                  cGTTACCCCACCAACAAGCTAATCTCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCtt                <  1:264689/70‑1 (MQ=255)
                                                    ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTgg             <  1:3529859/71‑1 (MQ=255)
                                                    ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTgg             >  1:3055949/1‑71 (MQ=255)
                                                      aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGcc                                >  1:166114/1‑50 (MQ=255)
                                                      aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTc           >  1:469849/1‑71 (MQ=39)
                                                       ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCg                              <  1:3584796/51‑1 (MQ=255)
                                                       ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCg                              <  1:4460798/51‑1 (MQ=255)
                                                       ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCt          <  1:3615093/71‑1 (MQ=255)
                                                        cccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAaga                                    <  1:2336716/44‑1 (MQ=255)
                                                        cccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAaga                                    <  1:3027657/44‑1 (MQ=255)
                                                         ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGTCAAGAGGCCCg                              <  1:2519365/49‑1 (MQ=255)
                                                         ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCg                              <  1:4026176/49‑1 (MQ=255)
                                                         ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCg                              <  1:2763391/49‑1 (MQ=255)
                                                            ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGc                                 >  1:4520048/1‑43 (MQ=255)
                                                            ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGc                                 >  1:801493/1‑43 (MQ=255)
                                                            ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGc                                 >  1:1339575/1‑43 (MQ=255)
                                                            ccaACAAGCTAATCCCATCTGGGCACATCAGATGGCAAGAGGCCCGAAGGTCCCCctct                 <  1:3667579/59‑1 (MQ=255)
                                                             caacaaGCTAATCCCATCTGGGCACATCCGATGGCa                                       <  1:763185/36‑1 (MQ=255)
                                                               acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGccc                               >  1:824054/1‑42 (MQ=38)
                                                               acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCtt         <  1:2742813/64‑1 (MQ=35)
                                                                caaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACAt  >  1:4151963/1‑70 (MQ=37)
                                                                 aaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCg                              >  1:1935655/1‑41 (MQ=32)
                                                                 |                                                                    
TGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACAT  >  Exported/1763529‑1763662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑