| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,012,045 | T→C | 9.5% | M63V (ATG→GTG) | yqhA ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,012,045 | 0 | T | C | 9.5% | 375.4 / 24.4 | 126 | M63V (ATG→GTG) | yqhA | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (85/29); new base C (5/7); total (90/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
AGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGT > Exported/2011985‑2012113 | agaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAg > 1:1462676/1‑70 (MQ=255)agaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGc > 1:2759495/1‑71 (MQ=255) aCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:506254/1‑71 (MQ=255) aCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:1497954/1‑71 (MQ=255) gAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:3205664/1‑69 (MQ=255) aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGTGTCATATCCACCAGCGa < 1:3394178/68‑1 (MQ=255) aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGTGTCATATCCACCAGCGACa > 1:1651762/1‑69 (MQ=255) aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAg > 1:175635/1‑70 (MQ=255) aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAg > 1:4198863/1‑70 (MQ=255) aaTACTCATAACCGGAAAACATCACAATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAg > 1:2219000/1‑70 (MQ=255) tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:4080880/1‑64 (MQ=255) tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:3262243/1‑64 (MQ=255) tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:628464/1‑64 (MQ=255) tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:3302592/1‑64 (MQ=255) tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:932347/1‑64 (MQ=255) ctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACa > 1:3801437/1‑65 (MQ=255) ctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAaca > 1:4088870/1‑71 (MQ=255) ctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAaca > 1:1868655/1‑71 (MQ=255) cATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCATCGACAg > 1:4527469/1‑64 (MQ=255) cATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAg > 1:1141269/1‑64 (MQ=255) aTAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGc < 1:2879033/64‑1 (MQ=255) aTAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAacac > 1:4313978/1‑69 (MQ=255) aTAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAacac > 1:2645462/1‑69 (MQ=255) tAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGc < 1:3655419/57‑1 (MQ=255) tAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAg < 1:2778132/71‑1 (MQ=255) tAACCGGAAAACAGCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAg < 1:2131302/71‑1 (MQ=255) aCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGa < 1:2526358/71‑1 (MQ=255) gAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCaa < 1:3961502/71‑1 (MQ=255) gAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCaa < 1:3971827/71‑1 (MQ=255) gAAAACATCACCATCACCAGCAAACCGCCAACCAGAGTCATATCCACAAGCGACATCAACACGAGGATCa > 1:3191435/1‑70 (MQ=255) aaaCATCACCATCACCAGCAAACCGCCAACCAGTGTCATATCCACCAGCGACAGCAACACGAGGATCAAAt < 1:1140128/71‑1 (MQ=255) aaaCATCACCATCACCAGCAAACCGCCAACCAGTGTCATATCCACCAGCGACAGCAACACGAGGATCAAAt < 1:158422/71‑1 (MQ=255) aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATc > 1:3219142/1‑71 (MQ=255) aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATc > 1:3213705/1‑71 (MQ=255) aCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCt < 1:860562/71‑1 (MQ=255) caccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:3765554/1‑71 (MQ=255) caccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:3541471/1‑71 (MQ=255) caccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:157914/1‑71 (MQ=255) caccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:3770804/1‑71 (MQ=255) ccatcaccaGCGAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATc > 1:439100/1‑59 (MQ=255) ccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATc > 1:338764/1‑59 (MQ=255) ccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATc > 1:3912630/1‑59 (MQ=255) ccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATc > 1:4121781/1‑59 (MQ=255) catcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCg < 1:3112923/71‑1 (MQ=255) catcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCg > 1:2075456/1‑71 (MQ=255) catcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCg > 1:639492/1‑71 (MQ=255) atcaccaGCAAACCGCCAACCAGTGTCATTTCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGc > 1:1591506/1‑71 (MQ=255) tcaccaGCAAACCGCCAACCAGTGTCATATCCACCAGCGa < 1:3547381/40‑1 (MQ=255) accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:2674905/1‑38 (MQ=255) accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:2411949/1‑38 (MQ=255) accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:3629075/1‑38 (MQ=255) accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc > 1:1648622/1‑65 (MQ=255) accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc > 1:3022031/1‑65 (MQ=255) accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCa < 1:1336589/70‑1 (MQ=255) accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCa < 1:2491093/70‑1 (MQ=255) accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCAt > 1:2216380/1‑71 (MQ=255) accaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCAt > 1:3824871/1‑71 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:1656996/1‑61 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:2926436/1‑61 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:1175518/1‑61 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:2600078/1‑61 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:4338291/1‑61 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:1336169/1‑61 (MQ=255) cAAACCGCCAACCAGTGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag < 1:4464659/70‑1 (MQ=255) cAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag > 1:2269188/1‑70 (MQ=255) cAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCga > 1:3875187/1‑69 (MQ=255) aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag > 1:1896346/1‑68 (MQ=255) aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag > 1:247022/1‑68 (MQ=255) aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag > 1:2893003/1‑68 (MQ=255) aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCAATCgag > 1:4081826/1‑68 (MQ=255) aCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag > 1:4135189/1‑67 (MQ=255) aCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTACGCCATCgag > 1:1176983/1‑67 (MQ=255) ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaaga > 1:3945739/1‑70 (MQ=255) ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaaga > 1:2082289/1‑70 (MQ=255) cGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata < 1:4623251/71‑1 (MQ=255) cGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata < 1:2712831/71‑1 (MQ=255) cGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata < 1:4290850/71‑1 (MQ=255) cGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata < 1:2106880/71‑1 (MQ=255) gCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGatat < 1:473954/71‑1 (MQ=255) ccAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCAt > 1:1924887/1‑59 (MQ=255) ccAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCAt > 1:4427190/1‑59 (MQ=255) ccAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATAtt > 1:2399744/1‑71 (MQ=255) ccAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATAtt > 1:3554284/1‑71 (MQ=255) aaCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg > 1:1483907/1‑71 (MQ=255) aaCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg > 1:3126428/1‑71 (MQ=255) aaCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg > 1:2235644/1‑71 (MQ=255) aaCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg > 1:3466646/1‑71 (MQ=255) aaCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg > 1:4752619/1‑71 (MQ=255) aaCCAGCGTCATATCCACCAGCGACAG‑AACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCgg < 1:1310173/71‑1 (MQ=255) aaCCAGCGTCATATACACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg > 1:4334288/1‑71 (MQ=255) aCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCgg < 1:4606468/71‑1 (MQ=255) ccAGTGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGc < 1:2347701/71‑1 (MQ=255) cAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGc < 1:4714079/70‑1 (MQ=255) gTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCa > 1:2743503/1‑49 (MQ=255) gTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc < 1:159564/71‑1 (MQ=255) gTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc < 1:970372/71‑1 (MQ=255) tCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACg > 1:562202/1‑71 (MQ=255) atatCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATAtt > 1:2049082/1‑59 (MQ=255) atatCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATAtt > 1:2139399/1‑59 (MQ=255) atatCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGt > 1:3263049/1‑70 (MQ=255) | AGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGT > Exported/2011985‑2012113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |