| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 151,149 | C→T | 11.2% | S560S (AGC→AGT) | mrcB → | fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 151,149 | 0 | C | T | 11.2% | 314.4 / 24.9 | 107 | S560S (AGC→AGT) | mrcB | fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (46/49); new base T (4/8); total (50/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
CAATTGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGC > Exported/151079‑151217 | catttGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGc < 1:4671489/68‑1 (MQ=255)cAATTGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCTTTATAGCGAAAGc < 1:1415414/71‑1 (MQ=255)cAATTGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGc < 1:2736954/71‑1 (MQ=255)cAATTGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGc < 1:593001/71‑1 (MQ=255) aTTGCGCTGCGTCAGCCGATTGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCgg > 1:3260021/1‑71 (MQ=255) aTTGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCgg > 1:4753990/1‑71 (MQ=255) aTTGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCgg < 1:1614441/71‑1 (MQ=255) aTTGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCgg < 1:3630070/71‑1 (MQ=255) ttGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGc < 1:1391452/71‑1 (MQ=255) cgcTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCa < 1:2798443/69‑1 (MQ=255) cTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGt < 1:3588487/71‑1 (MQ=255) tGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGt > 1:1118565/1‑70 (MQ=255) tGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGt > 1:4068668/1‑70 (MQ=255) cGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGa < 1:625269/70‑1 (MQ=255) cGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGa < 1:3159036/70‑1 (MQ=255) cGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGa < 1:3249490/70‑1 (MQ=255) cGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGa < 1:3401718/70‑1 (MQ=255) aGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCt < 1:3250555/70‑1 (MQ=255) aGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCt < 1:2061644/70‑1 (MQ=255) gCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCtgg > 1:2381181/1‑71 (MQ=255) gCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCtg < 1:487835/70‑1 (MQ=255) cagAATGGCCAGGTCTGTTCACCGCAGAATGATGACCGTCTTTATAGCGAAAGCGGCAGAGTGATTCt < 1:3521424/66‑1 (MQ=255) ctaaTGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCtggtg < 1:4446165/69‑1 (MQ=255) cGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGTGGCAGAGTGATGCtggtg < 1:941993/71‑1 (MQ=255) cGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGTGGCAGAGTGATGCtggtg < 1:2579455/71‑1 (MQ=255) gAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCtggtg > 1:302895/1‑70 (MQ=255) aTGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGTGGCAGAGTGATGCTGGTGGAt < 1:2195249/71‑1 (MQ=255) aTGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGTGGCAGAGTGATGCTGGTGGAt < 1:4459873/71‑1 (MQ=255) aTGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCa < 1:1002462/54‑1 (MQ=255) aTGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCa < 1:3749876/54‑1 (MQ=255) tGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATg < 1:835278/71‑1 (MQ=255) ggCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCaga > 1:2912037/1‑54 (MQ=255) ggCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCaga > 1:1883160/1‑54 (MQ=255) aGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGtt > 1:2146081/1‑70 (MQ=255) gTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGc < 1:911168/55‑1 (MQ=255) tCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGAcc < 1:3147585/71‑1 (MQ=255) tCACCGCAGAATGATGACCGTCGTTATAGCGAAAGTGGCAGTGTGATGCt < 1:640632/50‑1 (MQ=255) tCACCGCAGAATGATGACCGTCGTTATAGCGAAAGTGGCAGAGTGATGCt < 1:1882979/50‑1 (MQ=255) cACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTc > 1:297124/1‑70 (MQ=255) aCCGCAGAATGATGCCCGCCGTTATAGCGAAAGTGGCAGAGTGATGCtgg > 1:2373788/1‑50 (MQ=38) aCCGCAGAATGATGACCGTCGTTATAGCGAAAGTGGCAGAGTGATGCtgg > 1:2448037/1‑50 (MQ=255) aCCGCAGAATGATGACCGTCGTTATAGCGAAAGTGGCAGAGTGATGCtgg > 1:4148988/1‑50 (MQ=255) aCCGCAGAATGATGACCGTCGTTATAGCGAAAGTGGCAGAGTGATGCtgg > 1:3004012/1‑50 (MQ=255) aCCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGa > 1:4565386/1‑44 (MQ=255) aCCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGa < 1:1071491/71‑1 (MQ=255) aCCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGa < 1:4624409/71‑1 (MQ=255) cGCAGAATGATGCCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGa > 1:4051000/1‑69 (MQ=255) cGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGAt < 1:888864/43‑1 (MQ=255) cGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGa > 1:4220000/1‑69 (MQ=255) cGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGATGACCCGTTCGAt > 1:1676623/1‑70 (MQ=255) cAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGaa > 1:2013943/1‑71 (MQ=255) cAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGaa < 1:2892100/71‑1 (MQ=255) cAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGaa > 1:2244127/1‑71 (MQ=255) gAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACg < 1:1094299/71‑1 (MQ=255) gAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACg < 1:4163343/71‑1 (MQ=255) atgatgACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTg < 1:3436727/71‑1 (MQ=255) atgatgACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTg > 1:4569249/1‑71 (MQ=255) tgatgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCtgg > 1:4199160/1‑41 (MQ=255) tgatgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCt > 1:515876/1‑39 (MQ=255) tgatgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGa > 1:1750067/1‑53 (MQ=255) tgatgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGa > 1:910252/1‑53 (MQ=255) atgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGcc > 1:1388856/1‑70 (MQ=255) atgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGcc < 1:3413504/70‑1 (MQ=255) atgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGcc < 1:632981/70‑1 (MQ=255) atgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGcc < 1:801009/70‑1 (MQ=255) tgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACg < 1:3255817/65‑1 (MQ=255) tgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGa > 1:2933400/1‑71 (MQ=255) tgaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGa > 1:1709132/1‑71 (MQ=255) gaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCtggtg < 1:4240263/39‑1 (MQ=255) gaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCtggtg < 1:3461797/39‑1 (MQ=255) gaCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCtggtg < 1:3767712/39‑1 (MQ=255) aCCGTCGTTATAGCGAAAGCGGCAGAGTGATGCtggtg < 1:4044085/38‑1 (MQ=255) ccGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGa > 1:82956/1‑39 (MQ=255) ccGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGa > 1:3671163/1‑39 (MQ=255) ccGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGa > 1:3772013/1‑39 (MQ=255) tcgtTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCg > 1:1627160/1‑52 (MQ=255) tcgtTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTaa < 1:1431693/71‑1 (MQ=255) gtTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTaaa > 1:1228043/1‑70 (MQ=255) gtTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTaaa > 1:911544/1‑70 (MQ=255) gtTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTaaa > 1:322577/1‑70 (MQ=255) gtTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAAt > 1:1977968/1‑71 (MQ=255) gtTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAAt > 1:1333776/1‑71 (MQ=255) gtTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAAt > 1:2261795/1‑71 (MQ=255) gtTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAAt > 1:355259/1‑71 (MQ=255) tataGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCt > 1:2416707/1‑71 (MQ=255) ataGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCt < 1:2425130/70‑1 (MQ=255) ataGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTg > 1:4696718/1‑71 (MQ=255) gCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTggg < 1:4560212/70‑1 (MQ=255) cGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTgggg < 1:4645103/70‑1 (MQ=255) cGAAAGCGGCAGAGTGATGCTCGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTgggg < 1:1903808/70‑1 (MQ=255) gAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGt < 1:4575097/59‑1 (MQ=255) gAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGt < 1:3663154/59‑1 (MQ=255) aaGTGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATgg < 1:2102240/71‑1 (MQ=255) aaGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTaa > 1:1652632/1‑59 (MQ=255) aaGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTaa > 1:1642131/1‑59 (MQ=255) aGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGgc < 1:1739365/71‑1 (MQ=255) aGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGgc > 1:1783473/1‑71 (MQ=255) aGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGgc > 1:767684/1‑71 (MQ=255) aGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGgc < 1:3919774/71‑1 (MQ=255) aGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGgc > 1:4398814/1‑71 (MQ=255) | CAATTGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAATGATGACCGTCGTTATAGCGAAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGC > Exported/151079‑151217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |