| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,061,569 | G→A | 6.6% | A26T (GCA→ACA) ‡ | rpsU → | 30S ribosomal subunit protein S21 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,061,569 | 0 | G | A | 6.6% | 244.8 / 5.2 | 75 | A26T (GCA→ACA) ‡ | rpsU | 30S ribosomal subunit protein S21 |
| Reads supporting (aligned to +/- strand): ref base G (40/30); new base A (3/2); total (43/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGC > Exported/2061504‑2061634 | ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAgg < 1:1864572/70‑1 (MQ=255)ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgt > 1:711071/1‑71 (MQ=255) tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg > 1:1850508/1‑71 (MQ=255) tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg > 1:307214/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:364969/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:4416356/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:3040003/1‑71 (MQ=255) aaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2317958/70‑1 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGc > 1:127102/1‑59 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGc > 1:2404828/1‑59 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTgg < 1:1992416/70‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2518852/63‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:1463528/63‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:880993/63‑1 (MQ=255) gAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg > 1:2811013/1‑70 (MQ=255) aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2022568/61‑1 (MQ=255) aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg > 1:285253/1‑69 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa > 1:1019933/1‑71 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa > 1:1449614/1‑71 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGa > 1:432435/1‑70 (MQ=255) aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac < 1:3206717/71‑2 (MQ=255) aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac < 1:1410787/71‑2 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:987020/71‑1 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt > 1:283093/1‑71 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:4660032/71‑1 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:4741940/71‑1 (MQ=255) gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1479754/71‑1 (MQ=255) gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1336043/71‑1 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCTTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:4333830/1‑70 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTc > 1:375935/1‑50 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:544467/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:353370/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:946537/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:2890048/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:2437457/1‑70 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt < 1:3036209/70‑1 (MQ=255) gACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTg < 1:4286524/70‑1 (MQ=255) tAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGtt < 1:2920429/44‑1 (MQ=255) aGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGt > 1:3257521/1‑68 (MQ=255) aGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTc > 1:1869581/1‑70 (MQ=255) gCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCg < 1:3799372/48‑1 (MQ=255) gCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTa < 1:1819439/71‑1 (MQ=255) gCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGAGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCt < 1:4684452/70‑1 (MQ=255) tctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTAt < 1:718194/70‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1663780/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1307541/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:867195/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:2316137/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:173323/58‑1 (MQ=255) cGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:2984343/1‑70 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:2857210/1‑53 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTTGTGAGTTCTATGAAAAACCg < 1:4737592/71‑1 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:3080315/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:735017/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:4228349/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:1500118/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:4551479/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGCAAAAcc > 1:102703/1‑70 (MQ=255) ttCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAAcc < 1:1057783/68‑1 (MQ=255) cAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTa > 1:1024470/1‑71 (MQ=255) cAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTa > 1:422836/1‑71 (MQ=255) aGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAAc > 1:4381512/1‑63 (MQ=255) aGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTAcc > 1:1270394/1‑71 (MQ=255) gCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCg < 1:926129/71‑1 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTc > 1:2343183/1‑47 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGa > 1:4254704/1‑52 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACg > 1:1597642/1‑70 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACg > 1:3127679/1‑70 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACg > 1:2985056/1‑70 (MQ=255) cTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt < 1:3887087/40‑1 (MQ=255) cTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGt < 1:1482646/70‑1 (MQ=255) cTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGt < 1:1339688/70‑1 (MQ=255) tGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGGTCTATGAAAAACCGACTACCGAACGt > 1:1083863/1‑69 (MQ=255) gCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAg > 1:4242072/1‑71 (MQ=255) aaaaaGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAgcgc > 1:2868685/1‑71 (MQ=255) | TTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGC > Exported/2061504‑2061634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |