| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,061,575 | G→C | 9.5% | V28L (GTT→CTT) ‡ | rpsU → | 30S ribosomal subunit protein S21 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,061,575 | 0 | G | C | 9.5% | 180.0 / 7.9 | 63 | V28L (GTT→CTT) ‡ | rpsU | 30S ribosomal subunit protein S21 |
| Reads supporting (aligned to +/- strand): ref base G (32/25); new base C (3/3); total (35/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCT > Exported/2061505‑2061643 | tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg > 1:1850508/1‑71 (MQ=255)tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg > 1:307214/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:4416356/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:3040003/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:364969/1‑71 (MQ=255) aaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2317958/70‑1 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTgg < 1:1992416/70‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:880993/63‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:1463528/63‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2518852/63‑1 (MQ=255) gAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg > 1:2811013/1‑70 (MQ=255) aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2022568/61‑1 (MQ=255) aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg > 1:285253/1‑69 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa > 1:1019933/1‑71 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa > 1:1449614/1‑71 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGa > 1:432435/1‑70 (MQ=255) aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac < 1:3206717/71‑2 (MQ=255) aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac < 1:1410787/71‑2 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:987020/71‑1 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt > 1:283093/1‑71 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:4660032/71‑1 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:4741940/71‑1 (MQ=255) gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1336043/71‑1 (MQ=255) gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1479754/71‑1 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCTTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:4333830/1‑70 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTc > 1:375935/1‑50 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:353370/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:946537/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:544467/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:2890048/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt < 1:3036209/70‑1 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:2437457/1‑70 (MQ=255) gACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTg < 1:4286524/70‑1 (MQ=255) tAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGtt < 1:2920429/44‑1 (MQ=255) aGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGt > 1:3257521/1‑68 (MQ=255) aGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTc > 1:1869581/1‑70 (MQ=255) gCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCg < 1:3799372/48‑1 (MQ=255) gCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTa < 1:1819439/71‑1 (MQ=255) gCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGAGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCt < 1:4684452/70‑1 (MQ=255) tctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTAt < 1:718194/70‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:867195/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1307541/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1663780/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:2316137/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:173323/58‑1 (MQ=255) cGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:2984343/1‑70 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:2857210/1‑53 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTTGTGAGTTCTATGAAAAACCg < 1:4737592/71‑1 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:4551479/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:4228349/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:3080315/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:1500118/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:735017/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGCAAAAcc > 1:102703/1‑70 (MQ=255) ttCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAAcc < 1:1057783/68‑1 (MQ=255) cAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTa > 1:422836/1‑71 (MQ=255) cAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTa > 1:1024470/1‑71 (MQ=255) aGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAAc > 1:4381512/1‑63 (MQ=255) aGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTAcc > 1:1270394/1‑71 (MQ=255) gCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCg < 1:926129/71‑1 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTc > 1:2343183/1‑47 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGa > 1:4254704/1‑52 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACg > 1:2985056/1‑70 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACg > 1:3127679/1‑70 (MQ=255) ccTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACg > 1:1597642/1‑70 (MQ=255) cTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt < 1:3887087/40‑1 (MQ=255) cTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGt < 1:1482646/70‑1 (MQ=255) cTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGt < 1:1339688/70‑1 (MQ=255) tGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGGTCTATGAAAAACCGACTACCGAACGt > 1:1083863/1‑69 (MQ=255) gCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAg > 1:4242072/1‑71 (MQ=255) aaaaaGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAgcgc > 1:2868685/1‑71 (MQ=255) ggTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGc < 1:3800613/71‑1 (MQ=255) ggTCTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGc < 1:4652046/71‑1 (MQ=255) gtgtTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAgcgcgc < 1:1193951/64‑1 (MQ=255) gtgtTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCt < 1:102228/71‑1 (MQ=255) gtTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAg > 1:3362749/1‑67 (MQ=255) | TAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCT > Exported/2061505‑2061643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |