Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,892,0191.G100.0% 178.1 / NA 50intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/24);  total (26/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*Exported1,892,0192.C100.0% 179.7 / NA 50intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/24);  total (26/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  Exported/1891952‑1892077
                                                                    ||                                                          
tGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagc                                                            >  1:1047407/1‑68 (MQ=255)
 ggCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                           >  1:1955057/1‑67 (MQ=255)
  gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                           >  1:2597459/1‑66 (MQ=255)
  gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                           >  1:3258401/1‑66 (MQ=255)
  gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                           >  1:2117323/1‑66 (MQ=255)
            gAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               >  1:3554526/1‑71 (MQ=255)
            gAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               >  1:1437763/1‑71 (MQ=255)
              aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTTAACATATCAGCTAAGCACAGAACCgg                                             >  1:1361267/1‑71 (MQ=255)
              aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:586412/1‑68 (MQ=255)
               aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCggg                                            <  1:1302965/71‑1 (MQ=255)
                aGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:845026/1‑66 (MQ=255)
                 gattcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc                                          >  1:736751/1‑71 (MQ=255)
                 gattcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc                                          >  1:1339007/1‑71 (MQ=255)
                      gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     <  1:2114789/71‑1 (MQ=255)
                       attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                                    <  1:1816006/71‑1 (MQ=255)
                          cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:2796977/54‑1 (MQ=255)
                          cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:3157930/54‑1 (MQ=255)
                          cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:1942245/54‑1 (MQ=255)
                          cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  >  1:1419959/1‑70 (MQ=255)
                          cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  >  1:1879036/1‑70 (MQ=255)
                          cAGCACCGCTAAAACGACATTTACAGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  >  1:3616899/1‑70 (MQ=255)
                            gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                               <  1:174348/71‑1 (MQ=255)
                            gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                               <  1:1132364/71‑1 (MQ=255)
                                cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                           <  1:1931018/71‑1 (MQ=255)
                                 gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgcg                          >  1:1013112/1‑71 (MQ=255)
                                  cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         <  1:2316536/71‑1 (MQ=255)
                                     aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc                      <  1:1296011/71‑1 (MQ=255)
                                     aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                        <  1:1383438/69‑1 (MQ=255)
                                     aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         >  1:2247141/1‑68 (MQ=255)
                                       aagACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         >  1:2978533/3‑66 (MQ=255)
                                       aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         >  1:1773647/1‑66 (MQ=255)
                                       aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAt                    <  1:2976698/71‑1 (MQ=255)
                                         gACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                           <  1:2529721/62‑1 (MQ=255)
                                         gACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                           <  1:1709083/62‑1 (MQ=255)
                                           cATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         >  1:3215185/1‑62 (MQ=255)
                                           cATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         >  1:3014195/1‑62 (MQ=255)
                                            aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     <  1:1695954/49‑1 (MQ=255)
                                            aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     <  1:258177/49‑1 (MQ=255)
                                              ttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     >  1:937765/1‑47 (MQ=255)
                                                aCCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc                                          >  1:2234444/1‑40 (MQ=255)
                                                aCCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                       >  1:2142315/1‑59 (MQ=255)
                                                aCCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa           >  1:3274914/1‑71 (MQ=39)
                                                  cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGAtt         <  1:1676588/71‑1 (MQ=255)
                                                   gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg                 >  1:1357927/1‑62 (MQ=38)
                                                   gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTg        <  1:2417897/71‑1 (MQ=39)
                                                   gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTg        <  1:217339/71‑1 (MQ=39)
                                                    ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  >  1:3724451/1‑44 (MQ=37)
                                                    ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  >  1:1075146/1‑44 (MQ=37)
                                                      cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc                   >  1:2053972/1‑57 (MQ=255)
                                                      cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGCCCTGATTGtt      >  1:2822935/1‑70 (MQ=255)
                                                      cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGtt      >  1:3064958/1‑70 (MQ=38)
                                                       gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt     <  1:3363650/70‑1 (MQ=38)
                                                       gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGtt      <  1:529233/69‑1 (MQ=255)
                                                        ctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGt       <  1:2722227/67‑1 (MQ=255)
                                                           aaCATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAc  <  1:1926956/69‑1 (MQ=38)
                                                                    ||                                                          
TGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  Exported/1891952‑1892077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: